Sensitive high performance liquid chromatographic assay for assessment of doxorubicin pharmacokinetics in mouse plasma and tissues

• Published in: Journal of chromatography. B, Analytical technologies in the biomedical and life sciences Vol. 877; no. 8; pp. 837 - 841
• Main Authors: Urva, Shweta R., Shin, Beom Soo, Yang, Victor C., Balthasar, Joseph P.
• Format: Journal Article
• Language: English
• Published: Amsterdam Elsevier B.V 15.03.2009; Elsevier
• Subjects: Analysis; Analytical, structural and metabolic biochemistry; Animals; Biological and medical sciences; Chromatography, High Pressure Liquid - methods; Doxorubicin; Doxorubicin - blood; Doxorubicin - pharmacokinetics; Fundamental and applied biological sciences. Psychology; General pharmacology; HPLC; Male; Medical sciences; Mice; Mice, Nude; Mouse plasma; Pharmacology. Drug treatments; Protein precipitation; Sensitivity and Specificity; Tissues; Protein precipitation; Tissues; Doxorubicin; HPLC; Mouse plasma; Antineoplastic agent; Biological fluid; HPLC chromatography; Blood plasma; Tissue; Isomerases; DNA topoisomerase (ATP-hydrolysing); Evaluation; Chemical precipitation; Enzyme; Rodentia; Enzyme inhibitor; Topoisomerase II inhibitor; Protein; Vertebrata; Antibiotic; Mammalia; Mouse; Animal; Anthracyclins; Pharmacokinetics

A sensitive high performance liquid chromatography method (HPLC) has been developed for the quantification of doxorubicin in mouse plasma and tissues. Samples of serum or tissue homogenates, 20 μl, were analyzed following a single step protein precipitation using perchloric acid (35%, v/v). Doxorubi...