Beta-galactosidase from Lactobacillus pentosus: purification, characterization and formation of galacto-oligosaccharides

• Published in: Biotechnology journal Vol. 5; no. 8; p. 838
• Main Authors: Maischberger, Thomas, Leitner, Elisabeth, Nitisinprasert, Sunee, Juajun, Onladda, Yamabhai, Montarop, Nguyen, Thu-Ha, Haltrich, Dietmar
• Format: Journal Article
• Language: English
• Published: Germany 01.08.2010
• Subjects: Ammonium Sulfate; Bacterial Proteins - chemistry; Bacterial Proteins - isolation & purification; Bacterial Proteins - metabolism; beta-Galactosidase - chemistry; beta-Galactosidase - isolation & purification; beta-Galactosidase - metabolism; Electrophoresis, Polyacrylamide Gel; Enzyme Stability; Food Technology; Galactans - biosynthesis; Galactans - chemistry; Galactans - metabolism; Galactose - metabolism; Glycosylation; Hydrogen-Ion Concentration; Kinetics; Lactobacillus - chemistry; Lactobacillus - enzymology; Lactose - chemistry; Lactose - metabolism; Nitrophenylgalactosides - chemistry; Nitrophenylgalactosides - metabolism; Substrate Specificity; Temperature

A novel heterodimeric beta-galactosidase with a molecular mass of 105 kDa was purified from crude cell extracts of the soil isolate Lactobacillus pentosus KUB-ST10-1 using ammonium sulphate fractionation followed by hydrophobic interaction and affinity chromatography. The electrophoretically homogen...