Oral immunization of mice with a probiotic Lactobacillus casei constitutively expressing the α-toxoid induces protective immunity against Clostridium perfringens α-toxin

• Published in: Virulence Vol. 10; no. 1; pp. 166 - 179
• Main Authors: Gao, Xuwen, Ma, Yingying, Wang, Zhuo, Bai, Jing, Jia, Shuo, Feng, Baohua, Jiang, Yanping, Cui, Wen, Tang, Lijie, Li, Yijing, Wang, Li, Xu, Yigang
• Format: Journal Article
• Language: English
• Published: United States Taylor & Francis 01.01.2019; Taylor & Francis Group
• Subjects: Administration, Oral; Animals; Antibodies, Bacterial - blood; Bacterial Toxins - genetics; Bacterial Toxins - immunology; Bacterial Vaccines - immunology; Calcium-Binding Proteins - genetics; Calcium-Binding Proteins - immunology; Clostridium Infections - immunology; Clostridium Infections - prevention & control; Clostridium perfringens; Cytokines - blood; Female; genetically engineered Lactobacillus casei; Immunity, Cellular; Immunogenicity, Vaccine; Lactobacillus casei - genetics; Mice; Mice, Inbred BALB C; oral immunization; Organisms, Genetically Modified - immunology; Probiotics; Research Paper; Type C Phospholipases - genetics; Type C Phospholipases - immunology; α-toxoid; α-toxoid; oral immunization; genetically engineered
• ISSN: 2150-5594; 2150-5608; 2150-5608
• DOI: 10.1080/21505594.2019.1582975

Clostridium perfringens α-toxin is one of the major virulence factors during C. perfringens infection, causing hemolysis of erythrocytes in various species. Here, genetically engineered Lactobacillus casei (pPG-α/L. casei 393) constitutively expressing the toxoid of C. perfringens α-toxin was generated and its immunogenicity in mice for induction of protective immunity against the α-toxin was evaluated via oral immunization. The α-toxoid was constitutively expressed by pPG-α/L. casei 393 without a specific inducer, as confirmed by western blotting, laser confocal microscopy, and flow cytometry. In an experiment on BALB/c mice to evaluate the oral immunogenicity of pPG-α/L. casei 393, significant levels of a specific secretory IgA (sIgA) antibody in the intestinal mucus and feces and an IgG antibody in the serum of the probiotic vaccine group were detected after booster immunization (p < 0.05) as compared with the pPG/L. casei 393 and PBS control groups. These antibodies effectively neutralized C. perfringens natural α-toxin. Moreover, significantly higher levels of cytokines IL-2, IL-4, IL-10, IL-12, IL-17, and interferon (IFN) γ in the serum and increased proliferation of spleen lymphocytes obtained from mice orally immunized with pPG-α/L. casei 393 were detected. With a commercial C. perfringens type A inactivated vaccine as a control, immune protection provided by the probiotic vaccine against C. perfringens α-toxin was evaluated, and 90% and 80% protection rates were observed, respectively. Therefore, strain pPG-α/L. casei 393 effectively elicited mucosal, humoral, and cellular immunity, suggesting that pPG-α/L. casei 393 is a promising candidate for development of a vaccine against C. perfringens α-toxin.