Human Adipose-Derived Stem Cells Combined with Nano-Hydrogel Promote Functional Recovery after Spinal Cord Injury in Rats

The treatment of spinal cord injury aims to reconstruct the fiber connection and restore the interrupted neural pathways. Adipose mesenchymal stem cells (ADSCs) can promote the recovery of motor functions in spinal cord injury. However, poor survival of ADSCs and leakage outside of the injury site a...

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Published inBiology (Basel, Switzerland) Vol. 11; no. 5; p. 781
Main Authors Li, Jianping, Ji, Zhisheng, Wang, Yu, Li, Tiantian, Luo, Jinghua, Li, Jun, Shi, Xueshuang, Li, Liming, He, Liumin, Wu, Wutian
Format Journal Article
LanguageEnglish
Published Switzerland MDPI AG 20.05.2022
MDPI
Subjects
adipose mesenchymal stem cells
Alanine
Aspartic acid
Axons
Cytokines
Hydrogels
Inflammation
Injuries
Laboratory animals
Liquid chromatography
Mass spectroscopy
Mesenchymal stem cells
Mesenchyme
Microenvironments
Myelin
nano-hydrogel
Peptides
RADA16-I
RADA16-RGD
Recovery of function
Regeneration
Self-assembly
Spinal cord injuries
spinal cord injury
Stem cell transplantation
Stem cells
transplantation
Tumor necrosis factor-TNF
United States > US
Switzerland
China
spinal cord injury
adipose mesenchymal stem cells
nano-hydrogel
RADA16-RGD
RADA16-I
transplantation
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Summary:The treatment of spinal cord injury aims to reconstruct the fiber connection and restore the interrupted neural pathways. Adipose mesenchymal stem cells (ADSCs) can promote the recovery of motor functions in spinal cord injury. However, poor survival of ADSCs and leakage outside of the injury site after local transplantation reduce the number of cells, which seriously attenuates the cumulative effect. We performed heterotopic transplantation on rats with severe spinal cord injury using human ADSCs loaded within self-assembly hydrogel RADA16-RGD (R: arginine; A: alanine; D: aspartic acid; G: glycine). Our results indicate that the combined transplantation of human ADSCs with RADA16-RGD improved the survival of ADSCs at the injured site. The inflammatory reaction was inhibited, with improved survival of the neurons and increased residual area of nerve fibers and myelin protein. The functional behaviors were promoted, as determined by the Basso, Beattie, and Bresnahan (BBB) locomotor rating scale score and electrophysiological measurements. ADSCs can promote the repair of spinal cord injury. This study provides new ideas for the treatment of spinal cord injury.
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These authors contributed equally to this work.
ISSN:2079-7737
2079-7737
DOI:10.3390/biology11050781