Identifying Dynamic Interactors of Protein Complexes by Quantitative Mass Spectrometry

• Published in: Molecular & cellular proteomics Vol. 7; no. 1; pp. 46 - 57
• Main Authors: Wang, Xiaorong, Huang, Lan
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 01.01.2008; American Society for Biochemistry and Molecular Biology
• Subjects: Amino Acid Sequence; Cell Line; Humans; Immunoblotting; Isotope Labeling; Mass Spectrometry - methods; Molecular Sequence Data; Peptides - chemistry; Peptides - isolation & purification; Peptides - metabolism; Proteasome Endopeptidase Complex - metabolism; Protein Interaction Mapping - methods; Proteins - chemistry; Proteins - isolation & purification; Proteins - metabolism; Reproducibility of Results; Time Factors

Dynamically interacting proteins associate and dissociate with their binding partners at high on/off rates. Although their identification is of great significance to proteomics research, lack of an efficient strategy to distinguish stable and dynamic interactors has hampered the efforts toward this...