Flow cytometric quantitation and characterization of the T-lymphocyte memory response to CMV in healthy donors

• Published in: Cytotherapy (Oxford, England) Vol. 4; no. 1; pp. 29 - 40
• Main Authors: Hensel, N., Melenhorst, J.J., Bradstock, K., Schwarer, A.P., Eniafe, R., Nakamura, R., Barrett, A.J.
• Format: Journal Article
• Language: English
• Published: England Elsevier Inc 01.01.2002; Informa UK Ltd
• Subjects: Adoptive Transfer - methods; Antigens, Viral - immunology; CMV; Cytomegalovirus; Cytomegalovirus - immunology; Flow Cytometry - methods; Hematopoietic Stem Cell Transplantation; Humans; Immunologic Memory; Interferon-gamma - biosynthesis; interferon-γ; intracellular cytokine; lymphocyte; T-Lymphocyte Subsets - classification; T-Lymphocyte Subsets - immunology; Tissue Donors; TNF-α; Tumor Necrosis Factor-alpha - biosynthesis; Cytomegalovirus; interferon-γ; TNF-α; intracellular cytokine; CMV; lymphocyte

Levels of circulating CMV Ag-specific lymphocytes determine CMV reactivation risk in immunocompromised individuals. Frequencies of T cells producing cytokines after stimulation by CMV Agweremeasured in hematopoietic stem-cell donors using flow cytometry. In seropositive individuals (n = 75) the mean...