Flow cytometric quantitation and characterization of the T-lymphocyte memory response to CMV in healthy donors
Levels of circulating CMV Ag-specific lymphocytes determine CMV reactivation risk in immunocompromised individuals. Frequencies of T cells producing cytokines after stimulation by CMV Agweremeasured in hematopoietic stem-cell donors using flow cytometry. In seropositive individuals (n = 75) the mean...
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| Published in | Cytotherapy (Oxford, England) Vol. 4; no. 1; pp. 29 - 40 |
|---|---|
| Main Authors | , , , , , , |
| Format | Journal Article |
| Language | English |
| Published |
England
Elsevier Inc
01.01.2002
Informa UK Ltd |
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| Online Access | Get full text |
| ISSN | 1465-3249 1477-2566 |
| DOI | 10.1080/146532402317251509 |
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| Abstract | Levels of circulating CMV Ag-specific lymphocytes determine CMV reactivation risk in immunocompromised individuals.
Frequencies of T cells producing cytokines after stimulation by CMV Agweremeasured in hematopoietic stem-cell donors using flow cytometry.
In seropositive individuals (n = 75) the mean number of CD81 (CD8bright, CD8dim) and CD4+ cells producing IFN-γ was respectively 3.1% (12.6/μL) and 0.38% (3.2/μL), over 10–fold higher than in seronegative subjects (n = 22). CMV stimulation induced tumor necrosis factor-α (TNF-α) and interferon-γ (IFN-γ) in both CD4+ and CD8+ cells (usually together), with a shift from memory- to effector-cell phenotype, while only a small proportion of CD41 cells produced IL-4. Although the normal range was wide, neither age, sex nor HLA type affected the frequency.
These quantitative studies and the recognition of CD4+ cells as potential effectors of CMV immunity are of relevance for immunotherapeutic approaches to prevent CMV disease after stem-cell transplantation. |
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| AbstractList | Levels of circulating CMV Ag-specific lymphocytes determine CMV reactivation risk in immunocompromised individuals.
Frequencies of T cells producing cytokines after stimulation by CMV Agweremeasured in hematopoietic stem-cell donors using flow cytometry.
In seropositive individuals (n = 75) the mean number of CD81 (CD8bright, CD8dim) and CD4+ cells producing IFN-γ was respectively 3.1% (12.6/μL) and 0.38% (3.2/μL), over 10–fold higher than in seronegative subjects (n = 22). CMV stimulation induced tumor necrosis factor-α (TNF-α) and interferon-γ (IFN-γ) in both CD4+ and CD8+ cells (usually together), with a shift from memory- to effector-cell phenotype, while only a small proportion of CD41 cells produced IL-4. Although the normal range was wide, neither age, sex nor HLA type affected the frequency.
These quantitative studies and the recognition of CD4+ cells as potential effectors of CMV immunity are of relevance for immunotherapeutic approaches to prevent CMV disease after stem-cell transplantation. Background Levels of circulating CMV Ag-specific lymphocytes determine CMV reactivation risk in immunocompromised individuals. Methods Frequencies of T cells producing cytokines after stimulation by CMV Ag were measured in hematopoietic stem-cell donors using flow cytometry. Results In seropositive individuals (n = 75) the mean number of CD8 sub(+) (CD8 sub(bright), CD8 sub(dim)) and CD4 sub(+) cells producing IFN-gamma was respectively 3.1% (12.6/muL) and 0.38% (3.2/muL), over 10-fold higher than in seronegative subjects (n = 22). CMV stimulation induced tumor necrosis factor-alpha (TNF-alpha) and interferon-gamma (IFN-gamma) in both CD4 sub(+) and CD8 sub(+) cells (usually together), with a shift from memory- to effector-cell phenotype, while only a small proportion of CD4 sub(+) cells produced IL-4. Although the normal range was wide, neither age, sex nor HLA type affected the frequency. Discussion These quantitative studies and the recognition of CD4 sub(+) cells as potential effectors of CMV immunity are of relevance for immunotherapeutic approaches to prevent CMV disease after stem-cell transplantation. Levels of circulating CMV Ag-specific lymphocytes determine CMV reactivation risk in immunocompromised individuals.BACKGROUNDLevels of circulating CMV Ag-specific lymphocytes determine CMV reactivation risk in immunocompromised individuals.Frequencies of T cells producing cytokines after stimulation by CMV Ag were measured in hematopoietic stem-cell donors using flow cytometry.METHODSFrequencies of T cells producing cytokines after stimulation by CMV Ag were measured in hematopoietic stem-cell donors using flow cytometry.In seropositive individuals (n = 75) the mean number of CD8(+) (CD8(bright), CD8(dim)) and CD4(+) cells producing IFN-gamma was respectively 3.1% (12.6/microL) and 0.38% (3.2/microL), over 10-fold higher than in seronegative subjects (n = 22). CMV stimulation induced tumor necrosis factor-alpha (TNF-alpha) and interferon-gamma (IFN-gamma) in both CD4(+) and CD8(+) cells (usually together), with a shift from memory- to effector-cell phenotype, while only a small proportion of CD4(+) cells produced IL-4. Although the normal range was wide, neither age, sex nor HLA type affected the frequency.RESULTSIn seropositive individuals (n = 75) the mean number of CD8(+) (CD8(bright), CD8(dim)) and CD4(+) cells producing IFN-gamma was respectively 3.1% (12.6/microL) and 0.38% (3.2/microL), over 10-fold higher than in seronegative subjects (n = 22). CMV stimulation induced tumor necrosis factor-alpha (TNF-alpha) and interferon-gamma (IFN-gamma) in both CD4(+) and CD8(+) cells (usually together), with a shift from memory- to effector-cell phenotype, while only a small proportion of CD4(+) cells produced IL-4. Although the normal range was wide, neither age, sex nor HLA type affected the frequency.These quantitative studies and the recognition of CD4(+) cells as potential effectors of CMV immunity are of relevance for immunotherapeutic approaches to prevent CMV disease after stem-cell transplantation.DISCUSSIONThese quantitative studies and the recognition of CD4(+) cells as potential effectors of CMV immunity are of relevance for immunotherapeutic approaches to prevent CMV disease after stem-cell transplantation. Background Levels of circulating CMV Ag-specific lymphocytes determine CMV reactivation risk in immunocompromised individuals. Methods Frequencies of T cells producing cytokines after stimulation by CMV Ag were measured in hematopoietic stem-cell donors using flow cytometry. Results In seropositive individuals (n = 75) the mean number of CD8 + (CD8 bright, CD8 dim) and CD4 + cells producing IFN- n was respectively 3.1% (12.6/ w L) and 0.38% (3.2/ w L), over 10-fold higher than in seronegative subjects (n = 22). CMV stimulation induced tumor necrosis factor- f (TNF- f) and interferon- n (IFN- n) in both CD4 + and CD8 + cells (usually together), with a shift from memory- to effector-cell phenotype, while only a small proportion of CD4 + cells produced IL-4. Although the normal range was wide, neither age, sex nor HLA type affected the frequency. Discussion These quantitative studies and the recognition of CD4 + cells as potential effectors of CMV immunity are of relevance for immunotherapeutic approaches to prevent CMV disease after stem-cell transplantation. Levels of circulating CMV Ag-specific lymphocytes determine CMV reactivation risk in immunocompromised individuals. Frequencies of T cells producing cytokines after stimulation by CMV Ag were measured in hematopoietic stem-cell donors using flow cytometry. In seropositive individuals (n = 75) the mean number of CD8(+) (CD8(bright), CD8(dim)) and CD4(+) cells producing IFN-gamma was respectively 3.1% (12.6/microL) and 0.38% (3.2/microL), over 10-fold higher than in seronegative subjects (n = 22). CMV stimulation induced tumor necrosis factor-alpha (TNF-alpha) and interferon-gamma (IFN-gamma) in both CD4(+) and CD8(+) cells (usually together), with a shift from memory- to effector-cell phenotype, while only a small proportion of CD4(+) cells produced IL-4. Although the normal range was wide, neither age, sex nor HLA type affected the frequency. These quantitative studies and the recognition of CD4(+) cells as potential effectors of CMV immunity are of relevance for immunotherapeutic approaches to prevent CMV disease after stem-cell transplantation. |
| Author | Schwarer, A.P. Nakamura, R. Bradstock, K. Hensel, N. Melenhorst, J.J. Eniafe, R. Barrett, A.J. |
| Author_xml | – sequence: 1 givenname: N. surname: Hensel fullname: Hensel, N. organization: Stem Cell Transplantation Section, Hematology Branch, National Heart, Lung and Blood Institute, National Institutes of Health, Bethesda MD, USA – sequence: 2 givenname: J.J. surname: Melenhorst fullname: Melenhorst, J.J. organization: Stem Cell Transplantation Section, Hematology Branch, National Heart, Lung and Blood Institute, National Institutes of Health, Bethesda MD, USA – sequence: 3 givenname: K. surname: Bradstock fullname: Bradstock, K. organization: Blood and Marrow Transplant Service, Department of Haematology, Westmead Hospital, New South Wales, Australia – sequence: 4 givenname: A.P. surname: Schwarer fullname: Schwarer, A.P. organization: Bone Marrow Transplant Programme, Alfred Hospital, Melbourne, Victoria, Australia – sequence: 5 givenname: R. surname: Eniafe fullname: Eniafe, R. organization: Stem Cell Transplantation Section, Hematology Branch, National Heart, Lung and Blood Institute, National Institutes of Health, Bethesda MD, USA – sequence: 6 givenname: R. surname: Nakamura fullname: Nakamura, R. organization: Stem Cell Transplantation Section, Hematology Branch, National Heart, Lung and Blood Institute, National Institutes of Health, Bethesda MD, USA – sequence: 7 givenname: A.J. surname: Barrett fullname: Barrett, A.J. organization: Stem Cell Transplantation Section, Hematology Branch, National Heart, Lung and Blood Institute, National Institutes of Health, Bethesda MD, USA |
| BackLink | https://www.ncbi.nlm.nih.gov/pubmed/11953039$$D View this record in MEDLINE/PubMed |
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| Snippet | Levels of circulating CMV Ag-specific lymphocytes determine CMV reactivation risk in immunocompromised individuals.
Frequencies of T cells producing cytokines... Background Levels of circulating CMV Ag-specific lymphocytes determine CMV reactivation risk in immunocompromised individuals. Methods Frequencies of T cells... Levels of circulating CMV Ag-specific lymphocytes determine CMV reactivation risk in immunocompromised individuals.BACKGROUNDLevels of circulating CMV... |
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| SubjectTerms | Adoptive Transfer - methods Antigens, Viral - immunology CMV Cytomegalovirus Cytomegalovirus - immunology Flow Cytometry - methods Hematopoietic Stem Cell Transplantation Humans Immunologic Memory Interferon-gamma - biosynthesis interferon-γ intracellular cytokine lymphocyte T-Lymphocyte Subsets - classification T-Lymphocyte Subsets - immunology Tissue Donors TNF-α Tumor Necrosis Factor-alpha - biosynthesis |
| Title | Flow cytometric quantitation and characterization of the T-lymphocyte memory response to CMV in healthy donors |
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