Genome Editing with mRNA Encoding ZFN, TALEN, and Cas9

• Published in: Molecular therapy Vol. 27; no. 4; pp. 735 - 746
• Main Authors: Zhang, Hong-Xia, Zhang, Ying, Yin, Hao
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 10.04.2019; Elsevier Limited; American Society of Gene & Cell Therapy
• Subjects: Animals; Biotechnology industry; Clinical trials; CRISPR; CRISPR-Associated Protein 9 - genetics; CRISPR-Cas Systems - genetics; Deoxyribonucleic acid; DNA; DNA - genetics; Drug Delivery Systems - methods; Efficiency; Engineering; Enzymes; Gene Editing - methods; Genome; Genome editing; Genomes; Humans; mRNA; Nuclease; Proteins; Review; RNA, Messenger - genetics; Target recognition; Transcription activator-like effector nucleases; Transcription Activator-Like Effector Nucleases - genetics; Transcription, Genetic; Zinc Finger Nucleases - genetics

Genome-editing technologies based on programmable nucleases have significantly broadened our ability to make precise and direct changes in the genomic DNA of various species, including human cells. Delivery of programmable nucleases into the target tissue or cell is one of the pressing challenges in...