Peptide mimics selected from immune sera using phage display technology can replace native antigens in the diagnosis of Epstein–Barr virus infection

• Published in: Protein engineering, design and selection Vol. 22; no. 2; pp. 85 - 91
• Main Authors: Casey, J.L., Coley, A.M., Parisi, K., Foley, M.
• Format: Journal Article
• Language: English
• Published: England Oxford University Press 01.02.2009; Oxford Publishing Limited (England)
• Subjects: Animals; Antibodies, Viral - immunology; Antibody Affinity; Antigens, Viral - genetics; Antigens, Viral - immunology; EBV; Epstein-Barr virus; Epstein-Barr Virus Infections - diagnosis; Epstein-Barr Virus Infections - immunology; Herpesvirus 4, Human - immunology; Humans; Immunoglobulin G - immunology; Immunoglobulin M - immunology; mimics; mimotopes; Molecular Mimicry - genetics; Original; Peptide Library; peptides; Peptides - genetics; Peptides - immunology; phage-display; Protein Binding; Rabbits; Sensitivity and Specificity; Serum Albumin, Bovine; phage-display; EBV; peptides; mimics; mimotopes

There is an expanding area of small molecule discovery, especially in the area of peptide mimetics. Peptide sequences can be used to substitute for the entire native antigen for use in diagnostic assays. Our approach is to select peptides that mimic epitopes of the natural immune response to Epstein...