Increased rectal microbial richness is associated with the presence of colorectal adenomas in humans

• Published in: The ISME Journal Vol. 6; no. 10; pp. 1858 - 1868
• Main Authors: Sanapareddy, Nina, Legge, Ryan M, Jovov, Biljana, McCoy, Amber, Burcal, Lauren, Araujo-Perez, Felix, Randall, Thomas A, Galanko, Joseph, Benson, Andrew, Sandler, Robert S, Rawls, John F, Abdo, Zaid, Fodor, Anthony A, Keku, Temitope O
• Format: Journal Article
• Language: English
• Published: London Nature Publishing Group UK 01.10.2012; Oxford University Press; Nature Publishing Group
• Subjects: 631/326/2565/855; 692/698/2741/2135; 692/699/67/1504/1885; 692/700/139; Adenoma; Adenoma - microbiology; Adenoma - pathology; Bacteria - classification; Bacteria - genetics; Biomedical and Life Sciences; Biopsy; Case-Control Studies; Colorectal cancer; Colorectal carcinoma; Colorectal Neoplasms - microbiology; Colorectal Neoplasms - pathology; Crohn's disease; Diets; Digestive tract; DNA, Bacterial - genetics; Ecology; Evolutionary Biology; Female; Genetics; Humans; Life Sciences; Male; Metagenome; Microbial Ecology; Microbial Genetics and Genomics; Microbiology; Middle Aged; Mucosa; Obesity; Original; original-article; Rectum; Rectum - microbiology; Rectum - pathology; RNA, Ribosomal, 16S - genetics; rRNA 16S; Sequence Analysis, DNA; Titanium; Ulcerative colitis; diagnostic; commensal bacteria; colon cancer; BMI
• ISSN: 1751-7362; 1751-7370; 1751-7370
• DOI: 10.1038/ismej.2012.43

Differences in the composition of the gut microbial community have been associated with diseases such as obesity, Crohn’s disease, ulcerative colitis and colorectal cancer (CRC). We used 454 titanium pyrosequencing of the V1–V2 region of the 16S rRNA gene to characterize adherent bacterial communities in mucosal biopsy samples from 33 subjects with adenomas and 38 subjects without adenomas (controls). Biopsy samples from subjects with adenomas had greater numbers of bacteria from 87 taxa than controls; only 5 taxa were more abundant in control samples. The magnitude of the differences in the distal gut microbiota between patients with adenomas and controls was more pronounced than that of any other clinical parameters including obesity, diet or family history of CRC. This suggests that sequence analysis of the microbiota could be used to identify patients at risk for developing adenomas.