Combined liver–cytokine humanization comes to the rescue of circulating human red blood cells

• Published in: Science (American Association for the Advancement of Science) Vol. 371; no. 6533; pp. 1019 - 1025
• Main Authors: Song, Yuanbin, Shan, Liang, Gbyli, Rana, Liu, Wei, Strowig, Till, Patel, Amisha, Fu, Xiaoying, Wang, Xiaman, Xu, Mina L., Gao, Yimeng, Qin, Ashley, Bruscia, Emanuela M., Tebaldi, Toma, Biancon, Giulia, Mamillapalli, Padmavathi, Urbonas, David, Eynon, Elizabeth, Gonzalez, David G., Chen, Jie, Krause, Diane S., Alderman, Jonathan, Halene, Stephanie, Flavell, Richard A.
• Format: Journal Article
• Language: English
• Published: United States The American Association for the Advancement of Science 05.03.2021
• Subjects: Anemia, Sickle Cell - blood; Animal models; Animals; Blood; Blood Circulation; Bone marrow; Cell density; Complement component C3; CRISPR; Cytokines; Cytokines - metabolism; Disease Models, Animal; Erythrocytes; Erythrocytes - cytology; Erythropoiesis; Erythropoiesis - genetics; Erythropoiesis - physiology; Female; Fetuses; Fumarylacetoacetase; Gene Deletion; Genetic engineering; Hematopoiesis; Hematopoietic Stem Cells - cytology; Hepatocytes; Humanization; Humans; Hydrolases - genetics; Immunodeficiency; In vivo methods and tests; Infectious diseases; Liver; Liver - physiology; Liver transplantation; Malaria; Mice; Mice, Mutant Strains; Middle Aged; Occlusion; Pathology; Sickle cell disease; Stem cell transplantation; Stem cells; Survival; Vector-borne diseases

The study of primary human red blood cell (huRBC) disorders such as sickle cell disease (SCD) and infectious diseases such as malaria has been hampered by a lack of in vivo models of human erythropoiesis. Song et al. transferred human fetal liver cells into MISTRG mice, which are immunodeficient and...