Use of Anti-Idiotype Immunosorbents to Isolate Circulating Antigen-Specific T Cell-Derived Molecules from Hyperimmune Sera
We immunized four different sheep with antigen-binding material found in the serum of BALB/c mice 4 days after primary immunization with sheep erythrocytes (SRBC). The resultant antibodies made by the sheep contained a specificity(ies) that appeared to react with a dominant idiotype present on SRBC-...
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Published in | Proceedings of the National Academy of Sciences - PNAS Vol. 80; no. 5; pp. 1435 - 1439 |
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Main Authors | , , , |
Format | Journal Article |
Language | English |
Published |
United States
National Academy of Sciences of the United States of America
01.03.1983
National Acad Sciences |
Subjects | |
Online Access | Get full text |
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Summary: | We immunized four different sheep with antigen-binding material found in the serum of BALB/c mice 4 days after primary immunization with sheep erythrocytes (SRBC). The resultant antibodies made by the sheep contained a specificity(ies) that appeared to react with a dominant idiotype present on SRBC-specific Lyt-2+T cells. The antiserum made by the sheep markedly inhibited the formation of antigen-specific rosettes by SRBC educated T cells but did not inhibit T cells educated to other heterologous erythrocytes from forming crossreacting rosettes with SRBC or specific rosettes with the homologous erythrocytes. The ``anti-Id serum'' was depleted of all activity against known immunoglobulin isotypes and light chains and then was used to isolate antigen-binding molecules from mice that were hyperimmunized with SRBC. The ShId+material so isolated could be divided into two main groups--one that expressed immunoglobulin determinants, and one that did not. The former represented 15-25% of the ShId+protein isolated and comprised a minority of the anti-SRBC antibody in the anti-SRBC serum; the latter group of proteins bound sheep glycophorin specifically and expressed constant region determinants found on a number of other antigen-specific T cell factors. These experiments suggest that antigen-binding molecules made by T cells display much less heterogeneity than do antibodies and also show that the serum of hyperimmune mice contains significant amounts of T cell-derived antigen-specific immunoregulatory molecules. |
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Bibliography: | ObjectType-Article-2 SourceType-Scholarly Journals-1 ObjectType-Feature-1 content type line 23 ObjectType-Article-1 ObjectType-Feature-2 |
ISSN: | 0027-8424 1091-6490 |
DOI: | 10.1073/pnas.80.5.1435 |