A putative Arabidopsis thaliana glycosyltransferase, At4g01220, which is closely related to three plant cell wall-specific xylosyltransferases, is differentially expressed spatially and temporally
▶ We characterized At4g01220, a putative Arabidopsis thaliana encoding glycosyltransferases of CAZy GT-family-77. ▶ Presented data that provide insights into the expression, regulation and sub-cellular location of At4g01220. ▶In silico expression analysis and array data suggests involvement in cell...
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Published in | Plant science (Limerick) Vol. 180; no. 3; pp. 470 - 479 |
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Main Authors | , , , , , |
Format | Journal Article |
Language | English |
Published |
Shannon
Elsevier Ireland Ltd
01.03.2011
[Ireland]: Elsevier Science Ireland Ltd Elsevier |
Subjects | |
Online Access | Get full text |
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Summary: | ▶ We characterized At4g01220, a putative Arabidopsis thaliana encoding glycosyltransferases of CAZy GT-family-77. ▶ Presented data that provide insights into the expression, regulation and sub-cellular location of At4g01220. ▶In silico expression analysis and array data suggests involvement in cell wall biosynthesis with pectin being a prime candidate.
Plant cell wall polysaccharides are amongst the most complex, heterogeneous and abundant bio-molecules on earth. This makes the biosynthetic enzymes, namely the glycosyltransferases and polysaccharide synthases, important research targets in plant science and biotechnology. As an initial step to characterize At4g01220, a putative Arabidopsis thaliana encoding glycosyltransferases in CAZy GT-family-77 that is similar to three known xylosyltransferases involved in the biosynthesis of the pectic polysaccharide, rhamnogalacturonan II, we conducted an expression analysis. In transgenic Arabidopsis thaliana plants containing a fusion between the At4g01220 promoter and the gusA reporter gene we found the expression to be spatially and developmentally regulated. Analysis of Nicotiana benthamiana transfected with the At2g01220::YFP fusion protein revealed that the fusion protein resided in a Brefeldin A-sensitive compartment consistent with a sub-cellular location in the Golgi apparatus. In addition, in silico expression analysis from the Genevestigator database revealed that At4g01220 was up-regulated upon treatment with isoxaben, an inhibitor of cellulose synthesis, which, together with a co-expression analysis that identified a number of plant cell wall co-related biosynthetic genes, suggests involvement in cell wall biosynthesis with pectin being a prime candidate. The data presented provide insights into the expression, sub-cellular location and regulation of At4g01220 under various conditions and may help elucidate its specific function. |
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Bibliography: | http://dx.doi.org/10.1016/j.plantsci.2010.11.002 ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
ISSN: | 0168-9452 1873-2259 |
DOI: | 10.1016/j.plantsci.2010.11.002 |