Rewritable multi-event analog recording in bacterial and mammalian cells

• Published in: Science (American Association for the Advancement of Science) Vol. 360; no. 6385
• Main Authors: Tang, Weixin, Liu, David R.
• Format: Journal Article
• Language: English
• Published: United States The American Association for the Advancement of Science 13.04.2018
• Subjects: Analog-Digital Conversion; Antibiotics; Bacteria; Bacterial Proteins; Cameras; Cassettes; Cell lineage; Computer Storage Devices; CRISPR; CRISPR-Associated Protein 9; CRISPR-Cas Systems; Data recorders; Data storage; Deoxyribonucleic acid; Dilution; DNA; DNA, Bacterial - genetics; DNA, Bacterial - metabolism; Editing; Electrophysiological recording; Endonucleases; Escherichia coli - genetics; Escherichia coli - metabolism; Escherichia coli - virology; Exposure; Gene Editing; Gene expression; Gene mapping; Gene sequencing; Genomics; HEK293 Cells; Humans; Intracellular; Mammalian cells; Mammals; Memory devices; Multiplexing; Nuclease; Nucleotide sequence; Nutrients; Plasmids; Plasmids - genetics; Plasmids - metabolism; Recorders; Recording; Recording instruments; Ribonucleic acid; RNA; RNA, Guide, CRISPR-Cas Systems - metabolism; Signal transduction; Signaling; Stimuli; Viruses; Wnt protein; Writing

Recording cellular events could advance our understanding of cellular history and responses to stimuli. The construction of intracellular memory devices, however, is challenging. Tang and Liu used Cas9 nucleases and base editors to record amplitude, duration, and order of stimuli as stable changes i...