A versatile gene trap to visualize and interrogate the function of the vertebrate proteome

• Published in: Genes & development Vol. 25; no. 21; pp. 2306 - 2320
• Main Authors: Trinh, Le A, Hochgreb, Tatiana, Graham, Matthew, Wu, David, Ruf-Zamojski, Frederique, Jayasena, Chathurani S, Saxena, Ankur, Hawk, Rasheeda, Gonzalez-Serricchio, Aidyl, Dixson, Alana, Chow, Elly, Gonzales, Constanza, Leung, Ho-Yin, Solomon, Ilana, Bronner-Fraser, Marianne, Megason, Sean G, Fraser, Scott E
• Format: Journal Article
• Language: English
• Published: United States Cold Spring Harbor Laboratory Press 01.11.2011
• Subjects: Animals; Bacterial Proteins - genetics; Danio rerio; Databases, Protein; Embryo, Nonmammalian; Genetic Vectors; Internet; Luminescent Proteins - genetics; Molecular Sequence Annotation; Mutation; Proteome; Proteomics - methods; Recombinant Fusion Proteins - genetics; Recombinant Fusion Proteins - metabolism; Resource/Methodology; Zebrafish

We report a multifunctional gene-trapping approach, which generates full-length Citrine fusions with endogenous proteins and conditional mutants from a single integration event of the FlipTrap vector. We identified 170 FlipTrap zebrafish lines with diverse tissue-specific expression patterns and dis...