Functional Analysis of Nuclear Estrogen Receptors in Zebrafish Reproduction by Genome Editing Approach

• Published in: Endocrinology (Philadelphia) Vol. 158; no. 7; pp. 2292 - 2308
• Main Authors: Lu, Huijie, Cui, Yong, Jiang, Liwen, Ge, Wei
• Format: Journal Article
• Language: English
• Published: Washington, DC Endocrine Society 01.07.2017; Oxford University Press
• Subjects: Animals; Animals, Genetically Modified; Cell proliferation; Cell surface receptors; Cells, Cultured; Chorion; CRISPR; CRISPR-Cas Systems; Danio rerio; Editing; Embryo, Nonmammalian; Endocrinology; Estrogen receptors; Estrogens; Female; Females; Fish; Fish reproduction; Folliculogenesis; Functional analysis; Gene Editing - methods; Genetic analysis; Genome editing; Genomes; Genomic analysis; Male; Males; Mutants; Nuclease; Phenotypes; Receptors; Receptors, Estrogen - genetics; Receptors, Estrogen - physiology; Redundancy; Reproduction; Reproduction - genetics; Sex reversal; Zebrafish; Zebrafish - embryology; Zebrafish - genetics; Zebrafish - physiology; Zebrafish Proteins - genetics; Zebrafish Proteins - physiology

Estrogens signal through both nuclear and membrane receptors with most reported effects being mediated via the nuclear estrogen receptors (nERs). Although much work has been reported on nERs in the zebrafish, there is a lack of direct genetic evidence for their functional roles and importance in rep...