Novel recombination system using Cre recombinase alpha complementation

• Published in: Biotechnology letters Vol. 29; no. 9; pp. 1315 - 1322
• Main Authors: Seidi, Azadeh, Mie, Masayasu, Kobatake, Eiry
• Format: Journal Article
• Language: English
• Published: Dordrecht Dordrecht : Springer Netherlands 01.09.2007; Springer; Springer Nature B.V
• Subjects: Biological and medical sciences; Biotechnology; Cre recombinase; Cytomegalovirus; Escherichia coli - physiology; Escherichia coli Proteins - genetics; Escherichia coli Proteins - metabolism; Fundamental and applied biological sciences. Psychology; Genes; Green Fluorescent Proteins - genetics; Integrases - genetics; Promoter Regions, Genetic - genetics; Protein Engineering - methods; Protein transduction; Recombinant Proteins - biosynthesis; Short-term expression; Temporal control; Toxicity; α-Complementation; Recombination; Temporal control; α-Complementation .Cre recombinase; Complementation; Transduction; Gene expression; Protein transduction .Short-term expression; Protein

A major limitation for the use of Cre recombinase is its toxicity and a lack of temporal control over its activity. We have developed a new recombination system using Cre recombinase α-complementation. Cre recombinase was divided and one fragment (β) was introduced into cells between two loxP sites...