Evaluation of an In-house indirect ELISA for detection of antibody against haemorrhagic septicemia in Asian elephants

• Published in: Journal of Microbiological Methods Vol. 134; pp. 30 - 34
• Main Authors: Tankaew, Pallop, Singh-La, Thawatchai, Titaram, Chatchote, Punyapornwittaya, Veerasak, Vongchan, Preeyanat, Sawada, Takuo, Sthitmatee, Nattawooti
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 01.03.2017; Elsevier BV
• Subjects: animal health; Animals; antibodies; Antibodies, Bacterial; Antibodies, Bacterial - blood; antibody detection; antigens; Asian elephant; Bayes Theorem; Bayesian analysis; Bayesian theory; blood serum; calves; Cattle; cattle diseases; diagnostic techniques; Elephantidae; Elephants; Enzyme-Linked Immunosorbent Assay; Enzyme-Linked Immunosorbent Assay - methods; Enzyme-Linked Immunosorbent Assay - veterinary; Haemorrhagic septicemia; heat; hemagglutination; Hemorrhagic Septicemia; Hemorrhagic Septicemia - diagnosis; Hemorrhagic Septicemia - microbiology; Hemorrhagic Septicemia - veterinary; India; Indirect ELISA; Pasteurella multocida; Pasteurella multocida - immunology; Pasteurella multocida - isolation & purification; peroxidase; probability; Rabbits; Sensitivity and Specificity; septicemia; seroprevalence; serotypes; Sri Lanka; Thailand; titration; wild animals; Thailand; Sri Lanka; India; Haemorrhagic septicemia; Asian elephant; Indirect ELISA; Bayesian analysis

Pasteurella multocida causes haemorrhagic septicemia in livestock and wild animals, including elephants. The disease has been reported in Asian elephants in India and Sri Lanka, but to date there have been no reported cases in Thailand. ELISA or indirect hemagglutination assays (IHA) have been demon...