Characterization of depth-related microbial communities in lake sediment by denaturing gradient gel electrophoresis of amplified 16S rRNA fragments

• Published in: Journal of environmental sciences (China) Vol. 20; no. 2; pp. 224 - 230
• Main Authors: ZHAO, Xingqing, YANG, Liuyan, YU, Zhenyang, PENG, Naiying, XIAO, Lin, YIN, Daqiang, QIN, Boqiang
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 01.02.2008
• Subjects: 16S; 16S rRNA; Acinetobacter; Bacillus; Bacteria - classification; Bacteria - genetics; Base Sequence; Brevibacillus; China; denaturing gradient gel electrophoresis (DGGE); Electrophoresis; Exiguobacterium; Fresh Water - microbiology; Freshwater; Geologic Sediments - microbiology; Hydrogen-Ion Concentration; Lake Taihu; microbial diversity; Molecular Sequence Data; Nitrogen; Oxygen; Phosphorus; Phylogeny; Polymerase Chain Reaction; RNA, Bacterial - genetics; RNA, Ribosomal, 16S - genetics; rRNA; sediment; vertical distribution; Water Microbiology; 微生物; 水质污染; China; 16S rRNA; vertical distribution; microbial diversity; denaturing gradient gel electrophoresis (DGGE); sediment; Lake Taihu

The characterization of microbial communities of different depth sediment samples was examined by a culture-independent method and compared with physicochemical parameters, those are organic matter （OM）, total nitrogen （TN）, total phosphorus （TP）, pH and redox potential （Eh）. Total genomic DNA was e...