Comparison of IgA, TNF-α and surface tension of the tear film in two different times of the day
The main goal was to study the biochemical composition of the tear film in two different times of the day. Tear samples were collected from 10 individuals, non contact lenses wearers, from the university population without pathologies. To assess daily variations in the tear film, samples were collec...
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| Published in | Contact lens & anterior eye Vol. 36; no. 3; pp. 140 - 145 |
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| Main Authors | , , , , |
| Format | Journal Article |
| Language | English |
| Published |
England
Elsevier Ltd
01.06.2013
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| Subjects | |
| Online Access | Get full text |
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| Abstract | The main goal was to study the biochemical composition of the tear film in two different times of the day.
Tear samples were collected from 10 individuals, non contact lenses wearers, from the university population without pathologies. To assess daily variations in the tear film, samples were collected twice in the day, one early in the morning and another in the evening using capillary tubes. Tear protein profile was analyzed by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE), tumor necrosis factor α (TNF-α) and immunoglobulin A (IgA) were quantified by Enzyme-Linked ImmunoSorbent Assay (ELISA). Tear film stability was obtained through measurements of Non-Invasive Break Up Time and tear surface tension was measured by obtaining the Langmuir isotherms.
The stability of the tear film was higher in the morning than in the afternoon; corresponding to a higher value of surface tension in the afternoon. Protein electrophoresis tear profile is variable during the day as IgA concentration decreased from morning to afternoon (p<0.05). TNF-α concentration also decreased, but there were not significant statistical differences (p=0.089).
We concluded that there are daily variations in the composition and properties of the tear film, indicating that changes occur without being caused by contact lenses wear or by the presence of ocular and systemic pathologies. Presence of TNF-α is not a direct indication of inflammatory pathology, since this cytokine was identified in relatively high amounts in subjects without inflammatory pathology. |
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| AbstractList | The main goal was to study the biochemical composition of the tear film in two different times of the day.
Tear samples were collected from 10 individuals, non contact lenses wearers, from the university population without pathologies. To assess daily variations in the tear film, samples were collected twice in the day, one early in the morning and another in the evening using capillary tubes. Tear protein profile was analyzed by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE), tumor necrosis factor α (TNF-α) and immunoglobulin A (IgA) were quantified by Enzyme-Linked ImmunoSorbent Assay (ELISA). Tear film stability was obtained through measurements of Non-Invasive Break Up Time and tear surface tension was measured by obtaining the Langmuir isotherms.
The stability of the tear film was higher in the morning than in the afternoon; corresponding to a higher value of surface tension in the afternoon. Protein electrophoresis tear profile is variable during the day as IgA concentration decreased from morning to afternoon (p<0.05). TNF-α concentration also decreased, but there were not significant statistical differences (p=0.089).
We concluded that there are daily variations in the composition and properties of the tear film, indicating that changes occur without being caused by contact lenses wear or by the presence of ocular and systemic pathologies. Presence of TNF-α is not a direct indication of inflammatory pathology, since this cytokine was identified in relatively high amounts in subjects without inflammatory pathology. Abstract Purpose The main goal was to study the biochemical composition of the tear film in two different times of the day. Methods Tear samples were collected from 10 individuals, non contact lenses wearers, from the university population without pathologies. To assess daily variations in the tear film, samples were collected twice in the day, one early in the morning and another in the evening using capillary tubes. Tear protein profile was analyzed by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE), tumor necrosis factor α (TNF-α) and immunoglobulin A (IgA) were quantified by Enzyme-Linked ImmunoSorbent Assay (ELISA). Tear film stability was obtained through measurements of Non-Invasive Break Up Time and tear surface tension was measured by obtaining the Langmuir isotherms. Results The stability of the tear film was higher in the morning than in the afternoon; corresponding to a higher value of surface tension in the afternoon. Protein electrophoresis tear profile is variable during the day as IgA concentration decreased from morning to afternoon ( p < 0.05). TNF-α concentration also decreased, but there were not significant statistical differences ( p = 0.089). Conclusion We concluded that there are daily variations in the composition and properties of the tear film, indicating that changes occur without being caused by contact lenses wear or by the presence of ocular and systemic pathologies. Presence of TNF-α is not a direct indication of inflammatory pathology, since this cytokine was identified in relatively high amounts in subjects without inflammatory pathology. The main goal was to study the biochemical composition of the tear film in two different times of the day.PURPOSEThe main goal was to study the biochemical composition of the tear film in two different times of the day.Tear samples were collected from 10 individuals, non contact lenses wearers, from the university population without pathologies. To assess daily variations in the tear film, samples were collected twice in the day, one early in the morning and another in the evening using capillary tubes. Tear protein profile was analyzed by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE), tumor necrosis factor α (TNF-α) and immunoglobulin A (IgA) were quantified by Enzyme-Linked ImmunoSorbent Assay (ELISA). Tear film stability was obtained through measurements of Non-Invasive Break Up Time and tear surface tension was measured by obtaining the Langmuir isotherms.METHODSTear samples were collected from 10 individuals, non contact lenses wearers, from the university population without pathologies. To assess daily variations in the tear film, samples were collected twice in the day, one early in the morning and another in the evening using capillary tubes. Tear protein profile was analyzed by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE), tumor necrosis factor α (TNF-α) and immunoglobulin A (IgA) were quantified by Enzyme-Linked ImmunoSorbent Assay (ELISA). Tear film stability was obtained through measurements of Non-Invasive Break Up Time and tear surface tension was measured by obtaining the Langmuir isotherms.The stability of the tear film was higher in the morning than in the afternoon; corresponding to a higher value of surface tension in the afternoon. Protein electrophoresis tear profile is variable during the day as IgA concentration decreased from morning to afternoon (p<0.05). TNF-α concentration also decreased, but there were not significant statistical differences (p=0.089).RESULTSThe stability of the tear film was higher in the morning than in the afternoon; corresponding to a higher value of surface tension in the afternoon. Protein electrophoresis tear profile is variable during the day as IgA concentration decreased from morning to afternoon (p<0.05). TNF-α concentration also decreased, but there were not significant statistical differences (p=0.089).We concluded that there are daily variations in the composition and properties of the tear film, indicating that changes occur without being caused by contact lenses wear or by the presence of ocular and systemic pathologies. Presence of TNF-α is not a direct indication of inflammatory pathology, since this cytokine was identified in relatively high amounts in subjects without inflammatory pathology.CONCLUSIONWe concluded that there are daily variations in the composition and properties of the tear film, indicating that changes occur without being caused by contact lenses wear or by the presence of ocular and systemic pathologies. Presence of TNF-α is not a direct indication of inflammatory pathology, since this cytokine was identified in relatively high amounts in subjects without inflammatory pathology. |
| Author | Puinhas, Ana Real Oliveira, M. Elisabete C.D. Castanheira, Elisabete M.S. Sampaio, Paula Lira, Madalena |
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| BackLink | https://www.ncbi.nlm.nih.gov/pubmed/23290099$$D View this record in MEDLINE/PubMed |
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| CitedBy_id | crossref_primary_10_1016_j_exer_2013_07_014 crossref_primary_10_1167_iovs_65_3_2 crossref_primary_10_1016_j_jtos_2013_04_004 crossref_primary_10_1016_j_clae_2017_12_005 crossref_primary_10_1016_j_optom_2014_12_003 crossref_primary_10_1016_j_clae_2022_101600 crossref_primary_10_1080_02713683_2019_1645182 crossref_primary_10_1016_j_exer_2022_109101 crossref_primary_10_2478_acph_2019_0054 crossref_primary_10_1155_2017_5763172 crossref_primary_10_1155_2016_1910694 crossref_primary_10_1097_ACI_0000000000000307 crossref_primary_10_1016_j_clae_2018_11_008 crossref_primary_10_1007_s40123_022_00556_1 crossref_primary_10_1007_s00417_024_06606_7 crossref_primary_10_17337_JMBI_2019_21_1_89 crossref_primary_10_1097_ICL_0000000000001036 crossref_primary_10_1080_03639045_2016_1238924 crossref_primary_10_1080_08164622_2021_1878815 crossref_primary_10_1097_OPX_0000000000001484 crossref_primary_10_18502_jovr_v18i4_14543 |
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| Copyright | 2012 British Contact Lens Association British Contact Lens Association Copyright © 2012 British Contact Lens Association. Published by Elsevier Ltd. All rights reserved. |
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| Keywords | Tear film IgA Surface tension TNF-α Langmuir isotherms |
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| Snippet | The main goal was to study the biochemical composition of the tear film in two different times of the day.
Tear samples were collected from 10 individuals, non... Abstract Purpose The main goal was to study the biochemical composition of the tear film in two different times of the day. Methods Tear samples were collected... The main goal was to study the biochemical composition of the tear film in two different times of the day.PURPOSEThe main goal was to study the biochemical... |
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| SubjectTerms | Adult Eye Proteins - analysis Female Humans IgA Immunoglobulin A - analysis Langmuir isotherms Male Ophthalmology Surface Tension Tear film Tears - chemistry Tears - metabolism Time Factors TNF-α Tumor Necrosis Factor-alpha - analysis |
| Title | Comparison of IgA, TNF-α and surface tension of the tear film in two different times of the day |
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