Development and standardization of an in-house indirect ELISA for detection of duck antibody to fowl cholera

• Published in: Journal of Microbiological Methods Vol. 142; pp. 10 - 14
• Main Authors: Poolperm, Pichayanut, Varinrak, Thanya, Kataoka, Yasushi, Tragoolpua, Khajornsak, Sawada, Takuo, Sthitmatee, Nattawooti
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 01.11.2017; Elsevier BV
• Subjects: Animals; antibodies; Antibodies, Bacterial; Antibodies, Bacterial - blood; Antibodies, Bacterial - immunology; antigens; Antigens, Bacterial; Antigens, Bacterial - immunology; Bayesian analysis; Bayesian theory; blood serum; chickens; Cholera; Cholera - diagnosis; Cholera - microbiology; Cholera - veterinary; diagnostic techniques; Duck; ducklings; Ducks; Enzyme-Linked Immunosorbent Assay; Enzyme-Linked Immunosorbent Assay - methods; Fowl cholera; goats; heat; hemagglutination; Hemagglutination Tests; Hemagglutination Tests - methods; Immunoglobulin G; Immunoglobulin G - blood; Immunoglobulin G - immunology; Indirect ELISA; Indirect hemagglutination; Pasteurella Infections; Pasteurella Infections - diagnosis; Pasteurella Infections - microbiology; Pasteurella Infections - veterinary; Pasteurella multocida; Pasteurella multocida - immunology; peroxidase; Poultry Diseases; Poultry Diseases - diagnosis; Poultry Diseases - microbiology; probability; Sensitivity and Specificity; Seroconversion; seroprevalence; titration; turkeys; Duck; Indirect hemagglutination; Fowl cholera; Bayesian analysis; Indirect ELISA

Serological tests, such as agglutination and indirect hemagglutination assay (IHA), have been used to identify antibodies against Pasteurella multocida in poultry sera, but none are highly sensitive. An enzyme-linked immunosorbent assays (ELISA) has been used with varying degrees of success in attem...