Mechanism of siRNA production by a plant Dicer-RNA complex in dicing-competent conformation

• Published in: Science (American Association for the Advancement of Science) Vol. 374; no. 6571; pp. 1152 - 1157
• Main Authors: Wang, Qian, Xue, Yan, Zhang, Laixing, Zhong, Zhenhui, Feng, Suhua, Wang, Changshi, Xiao, Lifan, Yang, Zhenlin, Harris, C. Jake, Wu, Zhe, Zhai, Jixian, Yang, Maojun, Li, Sisi, Jacobsen, Steven E., Du, Jiamu
• Format: Journal Article
• Language: English
• Published: United States The American Association for the Advancement of Science 26.11.2021
• Subjects: Adenosine; Arabidopsis - chemistry; Arabidopsis - genetics; Arabidopsis - metabolism; Arabidopsis Proteins - chemistry; Arabidopsis Proteins - genetics; Arabidopsis Proteins - metabolism; Biological activity; Biosynthesis; Cryoelectron Microscopy; DNA methylation; Domains; Eukaryotes; Models, Molecular; Mutagenesis; Nucleic Acid Conformation; Nucleotides; Phosphorylation; Protein Binding; Protein Conformation; Protein Domains; Proteins; Ribonuclease III; Ribonuclease III - chemistry; Ribonuclease III - genetics; Ribonuclease III - metabolism; Ribonucleic acid; RNA; RNA, Plant - chemistry; RNA, Plant - metabolism; RNA, Small Interfering - chemistry; RNA, Small Interfering - metabolism; siRNA; Substrates

In the biogenesis of small RNAs, the Dicer family endonucleases act as a molecular ruler to cut the substrate RNA into defined lengths. Wang et al . report a structure of the dicing-competent state of plant DICER LIKE PROTEIN 3 (DCL3) in complex with a pre–small interfering RNA (pre-siRNA). Toward o...