An optogenetic gene expression system with rapid activation and deactivation kinetics

• Published in: Nature chemical biology Vol. 10; no. 3; pp. 196 - 202
• Main Authors: Motta-Mena, Laura B, Reade, Anna, Mallory, Michael J, Glantz, Spencer, Weiner, Orion D, Lynch, Kristen W, Gardner, Kevin H
• Format: Journal Article
• Language: English
• Published: New York Nature Publishing Group US 01.03.2014; Nature Publishing Group
• Subjects: 13; 13/95; 631/1647/245; 631/337/572; 631/92/552; 631/92/96; 82; 96; 96/44; Activating Transcription Factors - radiation effects; Animals; Bacterial Proteins - genetics; Biochemical Engineering; Biochemistry; Bioorganic Chemistry; Cell Biology; Cell Line; Cellular biology; Chemistry; Chemistry/Food Science; Danio rerio; Deoxyribonucleic acid; DNA; Embryos; Gene expression; Gene Expression - genetics; Kinetics; Light; Mammals; Models, Biological; Optogenetics; Promoter Regions, Genetic; Proteins; RNA-Binding Proteins - metabolism; T-Lymphocytes - metabolism; Toxicity; Zebrafish - genetics

Optogenetic systems permit the temporal and spatial control of gene expression using light. A variant of the LOV domain–containing EL222 protein displays responsive blue light–gated transcriptional control of genes in zebrafish and in mammalian cell lines. Optogenetic gene expression systems can con...