Chemical markers for rumen methanogens and methanogenesis

• Published in: Animal (Cambridge, England) Vol. 7; no. s2; pp. 409 - 417
• Main Authors: McCartney, C. A., Bull, I. D., Dewhurst, R. J.
• Format: Journal Article
• Language: English
• Published: Cambridge, UK Cambridge University Press 01.06.2013; Elsevier
• Subjects: Animal Husbandry; Animals; Archaea; archaeol; Bacteriological Techniques - methods; Bacteriological Techniques - veterinary; beef; Biomarkers - metabolism; Cattle - metabolism; Cattle - microbiology; Cell Membrane - chemistry; cell membranes; dairy cattle; Dairying; detection limit; Diet; digesta; ecosystems; environmental impact; ether lipids; ethers; Euryarchaeota - metabolism; fatty acid composition; feces; Feces - microbiology; Female; genes; genetic variation; glycerol; Glyceryl Ethers - metabolism; herbivores; Lipid Metabolism; Male; metabolism; methane; Methane - metabolism; methane production; methanogens; milk; milk fatty acids; odd chain fatty acids; oligodeoxyribonucleotides; polymerase chain reaction; quantitative polymerase chain reaction; ribosomal RNA; rumen; Rumen - metabolism; Rumen - microbiology; rumen fermentation; urine; volatile fatty acids; archaeol; methane; ether lipids; polymerase chain reaction; archaea

The targeting of mcrA or 16S rRNA genes by quantitative PCR (qPCR) has become the dominant method for quantifying methanogens in rumen. There are considerable discrepancies between estimates based on different primer sets, and the literature is equivocal about the relationship with methane productio...