Antihypertensive effect of the bovine casein-derived peptide Met-Lys-Pro

• Published in: Food chemistry Vol. 172; pp. 441 - 446
• Main Authors: Yamada, Akio, Sakurai, Takuma, Ochi, Daisuke, Mitsuyama, Eri, Yamauchi, Koji, Abe, Fumiaki
• Format: Journal Article
• Language: English
• Published: England Elsevier Ltd 01.04.2015
• Subjects: 14C-labeled peptide; ACE-inhibitory peptide; Administration, Oral; Angiotensin I-converting enzyme; Angiotensin-Converting Enzyme Inhibitors - chemistry; Angiotensin-Converting Enzyme Inhibitors - pharmacology; Animals; Antihypertensive Agents - pharmacology; Bioavailability; Blood Pressure - drug effects; Casein; Caseins - chemistry; Caseins - pharmacology; Dose-Response Relationship, Drug; Hypertension; Male; Peptides - chemistry; Rats; Rats, Inbred SHR; Rats, Wistar; Vasoconstriction - drug effects; Hypertension; Ile-Pro-Pro (CID 24978506); Casein; Angiotensin I-converting enzyme; Val-Pro-Pro (CID 9818200); Val-Tyr (CID 4065033); Bioavailability; ACE-inhibitory peptide; 14C-labeled peptide; C-labeled peptide
• ISSN: 0308-8146; 1873-7072
• DOI: 10.1016/j.foodchem.2014.09.098

•The bovine casein-derived peptide Met-Lys-Pro (MKP) was examined in vitro and in vivo.•MKP showed angiotensin I-converting enzyme (ACE)-inhibitory activity in vitro.•Intact MKP could be absorbed into the plasma by the oral administration.•The MKP suppressed (and delayed) angiotensin II-dependent vasoconstriction.•The single-dose or daily administration of MKP to SHRs lowered blood pressure. The antihypertensive effect of the bovine casein-derived peptide Met-Lys-Pro (MKP) was examined in vitro and in vivo. MKP showed angiotensin I-converting enzyme (ACE)-inhibitory activity in vitro (IC50=0.43μM). An in vivo kinetics study using radiolabeled Met-[1-14C]Lys-Pro (14C-MKP) showed that orally administered 14C-MKP to spontaneously hypertensive rats (SHRs) was absorbed and moved into the plasma. In vitro vasoconstriction of thoracic aorta preparations, which was induced by adding angiotensin I, was reduced by prior exposure of MKP. A single oral dose of MKP lowered systolic blood pressure (SBP) of SHRs, and repeated oral administration of MKP for 28days significantly lowered SBP of SHRs. The results obtained in the present study suggest that orally administrated MKP can be absorbed into the plasma and its ACE-inhibitory activity may contribute to induce the antihypertensive effect in vivo.