Genome Characterisation of an Isoprene-Degrading Alcaligenes sp. Isolated from a Tropical Restored Forest

Isoprene is a climate-active biogenic volatile organic compound (BVOC), emitted into the atmosphere in abundance, mainly from terrestrial plants. Soil is an important sink for isoprene due to its consumption by microbes. In this study, we report the ability of a soil bacterium to degrade isoprene. S...

Full description

Saved in:
Bibliographic Details
Published inBiology (Basel, Switzerland) Vol. 11; no. 4; p. 519
Main Authors Uttarotai, Toungporn, Sutheeworapong, Sawannee, Crombie, Andrew T., Murrell, J. Colin, Mhuantong, Wuttichai, Noirungsee, Nuttapol, Wangkarn, Sunanta, Bovonsombut, Sakunnee, McGenity, Terry J., Chitov, Thararat
Format Journal Article
LanguageEnglish
Published Switzerland MDPI AG 28.03.2022
MDPI
Subjects
Alcaligenes
Bacteria
Biodegradation
Carbon
climate-active gas
Enzymes
Gas chromatography
genome
Genomes
Genomic analysis
Gram-positive bacteria
Greenhouse gases
Isoprene
isoprene degradation
Metabolic pathways
Metabolism
Monooxygenase
National parks
Organic compounds
Prunus cerasoides
Soil microorganisms
VOCs
Volatile organic compounds
United Kingdom > UK
Thailand
United States > US
genome
isoprene degradation
climate-active gas
isoprene
Alcaligenes
Online AccessGet full text

Cover

Abstract Isoprene is a climate-active biogenic volatile organic compound (BVOC), emitted into the atmosphere in abundance, mainly from terrestrial plants. Soil is an important sink for isoprene due to its consumption by microbes. In this study, we report the ability of a soil bacterium to degrade isoprene. Strain 13f was isolated from soil beneath wild Himalayan cherry trees in a tropical restored forest. Based on phylogenomic analysis and an Average Nucleotide Identity score of >95%, it most probably belongs to the species Alcaligenes faecalis. Isoprene degradation by Alcaligenes sp. strain 13f was measured by using gas chromatography. When isoprene was supplied as the sole carbon and energy source at the concentration of 7.2 × 105 ppbv and 7.2 × 106 ppbv, 32.6% and 19.6% of isoprene was consumed after 18 days, respectively. Genome analysis of Alcaligenes sp. strain 13f revealed that the genes that are typically found as part of the isoprene monooxygenase gene cluster in other isoprene-degrading bacteria were absent. This discovery suggests that there may be alternative pathways for isoprene metabolism.
AbstractList Isoprene is a climate-active biogenic volatile organic compound (BVOC), emitted into the atmosphere in abundance, mainly from terrestrial plants. Soil is an important sink for isoprene due to its consumption by microbes. In this study, we report the ability of a soil bacterium to degrade isoprene. Strain 13f was isolated from soil beneath wild Himalayan cherry trees in a tropical restored forest. Based on phylogenomic analysis and an Average Nucleotide Identity score of >95%, it most probably belongs to the species Alcaligenes faecalis. Isoprene degradation by Alcaligenes sp. strain 13f was measured by using gas chromatography. When isoprene was supplied as the sole carbon and energy source at the concentration of 7.2 × 105 ppbv and 7.2 × 106 ppbv, 32.6% and 19.6% of isoprene was consumed after 18 days, respectively. Genome analysis of Alcaligenes sp. strain 13f revealed that the genes that are typically found as part of the isoprene monooxygenase gene cluster in other isoprene-degrading bacteria were absent. This discovery suggests that there may be alternative pathways for isoprene metabolism.
Simple SummaryIsoprene, a volatile hydrocarbon, is the second most abundantly produced climate-active gas, with largely indirect detrimental impacts, such as extending the residence time of the greenhouse gas methane. Isoprene is mainly emitted by plants and can be consumed by a range of microbes inhabiting diverse environments, including soil. Here, the ability of soil bacteria to degrade isoprene was investigated. Soil samples were taken from beneath wild Himalayan cherry trees in a tropical restored forest area, and an Alcaligenes sp. (strain 13f) was isolated. This isolate used isoprene as a sole source of carbon and energy (32.6% of isoprene was consumed in 18 days). A surprising finding from the genome analysis of Alcaligenes sp. strain 13f was that the well-characterised genes and genetic organisation typical of other isoprene-degrading bacteria were not observed. Thus, we propose that this strain uses a different metabolic pathway for isoprene degradation.AbstractIsoprene is a climate-active biogenic volatile organic compound (BVOC), emitted into the atmosphere in abundance, mainly from terrestrial plants. Soil is an important sink for isoprene due to its consumption by microbes. In this study, we report the ability of a soil bacterium to degrade isoprene. Strain 13f was isolated from soil beneath wild Himalayan cherry trees in a tropical restored forest. Based on phylogenomic analysis and an Average Nucleotide Identity score of >95%, it most probably belongs to the species Alcaligenes faecalis. Isoprene degradation by Alcaligenes sp. strain 13f was measured by using gas chromatography. When isoprene was supplied as the sole carbon and energy source at the concentration of 7.2 × 105 ppbv and 7.2 × 106 ppbv, 32.6% and 19.6% of isoprene was consumed after 18 days, respectively. Genome analysis of Alcaligenes sp. strain 13f revealed that the genes that are typically found as part of the isoprene monooxygenase gene cluster in other isoprene-degrading bacteria were absent. This discovery suggests that there may be alternative pathways for isoprene metabolism.
Isoprene is a climate-active biogenic volatile organic compound (BVOC), emitted into the atmosphere in abundance, mainly from terrestrial plants. Soil is an important sink for isoprene due to its consumption by microbes. In this study, we report the ability of a soil bacterium to degrade isoprene. Strain 13f was isolated from soil beneath wild Himalayan cherry trees in a tropical restored forest. Based on phylogenomic analysis and an Average Nucleotide Identity score of >95%, it most probably belongs to the species Alcaligenes faecalis. Isoprene degradation by Alcaligenes sp. strain 13f was measured by using gas chromatography. When isoprene was supplied as the sole carbon and energy source at the concentration of 7.2 × 105 ppbv and 7.2 × 106 ppbv, 32.6% and 19.6% of isoprene was consumed after 18 days, respectively. Genome analysis of Alcaligenes sp. strain 13f revealed that the genes that are typically found as part of the isoprene monooxygenase gene cluster in other isoprene-degrading bacteria were absent. This discovery suggests that there may be alternative pathways for isoprene metabolism.Isoprene is a climate-active biogenic volatile organic compound (BVOC), emitted into the atmosphere in abundance, mainly from terrestrial plants. Soil is an important sink for isoprene due to its consumption by microbes. In this study, we report the ability of a soil bacterium to degrade isoprene. Strain 13f was isolated from soil beneath wild Himalayan cherry trees in a tropical restored forest. Based on phylogenomic analysis and an Average Nucleotide Identity score of >95%, it most probably belongs to the species Alcaligenes faecalis. Isoprene degradation by Alcaligenes sp. strain 13f was measured by using gas chromatography. When isoprene was supplied as the sole carbon and energy source at the concentration of 7.2 × 105 ppbv and 7.2 × 106 ppbv, 32.6% and 19.6% of isoprene was consumed after 18 days, respectively. Genome analysis of Alcaligenes sp. strain 13f revealed that the genes that are typically found as part of the isoprene monooxygenase gene cluster in other isoprene-degrading bacteria were absent. This discovery suggests that there may be alternative pathways for isoprene metabolism.
Author Sutheeworapong, Sawannee
Wangkarn, Sunanta
McGenity, Terry J.
Uttarotai, Toungporn
Mhuantong, Wuttichai
Murrell, J. Colin
Crombie, Andrew T.
Chitov, Thararat
Noirungsee, Nuttapol
Bovonsombut, Sakunnee
AuthorAffiliation 4 School of Environmental Sciences, University of East Anglia, Norwich NR4 7TJ, UK; a.crombie@uea.ac.uk (A.T.C.); j.c.murrell@uea.ac.uk (J.C.M.)
5 Enzyme Technology Research Team, Biorefinery and Bioproduct Technology Research Group, National Center for Genetic Engineering and Biotechnology, Pathumthani 12120, Thailand; wuttichai.mhu@biotec.or.th
1 Department of Biology, Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand; toungporn_u@cmu.ac.th (T.U.); nuttapol.n@cmu.ac.th (N.N.); sakunnee.b@cmu.ac.th (S.B.)
3 School of Bioresources and Technology, King Mongkut’s University of Technology Thonburi, Bangkok 10150, Thailand; sawannee.sut@kmutt.ac.th
7 Environmental Science Research Center (ESRC), Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand
6 Department of Chemistry, Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand; sunanta@chiangmai.ac.th
2 School of Life Sciences, University of Essex, Colchester CO4 3SQ, UK
AuthorAffiliation_xml – name: 5 Enzyme Technology Research Team, Biorefinery and Bioproduct Technology Research Group, National Center for Genetic Engineering and Biotechnology, Pathumthani 12120, Thailand; wuttichai.mhu@biotec.or.th
– name: 6 Department of Chemistry, Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand; sunanta@chiangmai.ac.th
– name: 3 School of Bioresources and Technology, King Mongkut’s University of Technology Thonburi, Bangkok 10150, Thailand; sawannee.sut@kmutt.ac.th
– name: 4 School of Environmental Sciences, University of East Anglia, Norwich NR4 7TJ, UK; a.crombie@uea.ac.uk (A.T.C.); j.c.murrell@uea.ac.uk (J.C.M.)
– name: 7 Environmental Science Research Center (ESRC), Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand
– name: 1 Department of Biology, Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand; toungporn_u@cmu.ac.th (T.U.); nuttapol.n@cmu.ac.th (N.N.); sakunnee.b@cmu.ac.th (S.B.)
– name: 2 School of Life Sciences, University of Essex, Colchester CO4 3SQ, UK
Author_xml – sequence: 1
  givenname: Toungporn
  surname: Uttarotai
  fullname: Uttarotai, Toungporn
– sequence: 2
  givenname: Sawannee
  surname: Sutheeworapong
  fullname: Sutheeworapong, Sawannee
– sequence: 3
  givenname: Andrew T.
  surname: Crombie
  fullname: Crombie, Andrew T.
– sequence: 4
  givenname: J. Colin
  surname: Murrell
  fullname: Murrell, J. Colin
– sequence: 5
  givenname: Wuttichai
  orcidid: 0000-0002-3355-0071
  surname: Mhuantong
  fullname: Mhuantong, Wuttichai
– sequence: 6
  givenname: Nuttapol
  surname: Noirungsee
  fullname: Noirungsee, Nuttapol
– sequence: 7
  givenname: Sunanta
  surname: Wangkarn
  fullname: Wangkarn, Sunanta
– sequence: 8
  givenname: Sakunnee
  surname: Bovonsombut
  fullname: Bovonsombut, Sakunnee
– sequence: 9
  givenname: Terry J.
  orcidid: 0000-0002-1497-8822
  surname: McGenity
  fullname: McGenity, Terry J.
– sequence: 10
  givenname: Thararat
  surname: Chitov
  fullname: Chitov, Thararat
BackLink https://www.ncbi.nlm.nih.gov/pubmed/35453719$$D View this record in MEDLINE/PubMed
BookMark eNp1kk1r3DAQhk1JadI0596KoZdenIy-LOtSCNsmXQgUSnoWY1l2tNjSVvIG8u8rZ9OQLFQHSWieeXlnNO-LIx-8LYqPBM4ZU3DRujCG4YEQ4CCIelOcUJCqkpLJoxf34-IspQ3kJYHWrH5XHDPBBZNEnRTu2vow2XJ1hxHNbKNLOLvgy9CX6Mt1Cttova2-2SFi5_xQXo4GRzfkx1Sm7fmCjDjbruxjmEosb2PYuoyUv2yaQ8yBq7yn-UPxtscx2bOn87T4ffX9dvWjuvl5vV5d3lSGN3KuiJUCOuAIPdSKCtPXSFpJbAsUgPeiaQR2qoaa9K2yOYQGWuSCWEqAMHZarPe6XcCN3kY3YXzQAZ1-fAhx0BhnZ0arBWspMyhyq3reEN4yYLThDBkxCjuetb7utba7drKdsX6OOL4SfR3x7k4P4V4rYECaJgt8eRKI4c8ud0FPLhk7juht2CVNa8Gpamq5-P58gG7CLvrcqoWiKhfOFkefXjp6tvLvRzNwsQdMDClF2z8jBPQyNvpgbHKGOMgwbn4cglySG_-b9xfvZseG
CitedBy_id crossref_primary_10_1111_1758_2229_13212
crossref_primary_10_1128_aem_02122_22
crossref_primary_10_1080_20565623_2024_2430452
crossref_primary_10_1186_s13065_023_01016_y
crossref_primary_10_1111_1462_2920_16325
crossref_primary_10_3390_pr13030916
Cites_doi 10.1128/AEM.66.5.1877-1882.2000
10.1128/JB.181.7.2094-2101.1999
10.1073/pnas.0409727102
10.1016/S0378-1127(03)00211-1
10.1093/molbev/msab199
10.1038/s41396-018-0072-6
10.3390/genes11020139
10.12952/journal.elementa.000053
10.1093/bioinformatics/bty121
10.1128/JB.182.7.1956-1963.2000
10.1093/molbev/msy096
10.1093/nar/gkt1099
10.1016/j.atmosenv.2009.09.002
10.1099/mic.0.000931
10.1016/j.syapm.2005.03.003
10.1111/j.1365-2486.2005.00986.x
10.1007/s10482-015-0540-1
10.1016/j.watres.2006.06.021
10.1128/jb.173.2.697-703.1991
10.1128/AEM.64.6.2032-2043.1998
10.1128/AEM.64.1.172-177.1998
10.5194/acp-8-4605-2008
10.1038/srep21332
10.5194/acp-8-1329-2008
10.1029/97GL02451
10.1007/BF00276540
10.1093/bioinformatics/btu170
10.1016/S0022-2836(05)80360-2
10.1111/1462-2920.13345
10.1099/ijs.0.64483-0
10.1093/nar/gkz239
10.3390/microorganisms8030349
10.1099/ijs.0.63849-0
10.1111/j.1462-2920.2009.02069.x
10.1016/j.biortech.2017.02.002
10.1128/AEM.64.8.2800-2805.1998
10.1111/1462-2920.12793
10.1128/AEM.00154-18
10.1111/j.1365-2486.2006.01112.x
10.1128/AEM.66.12.5488-5491.2000
10.1371/journal.pone.0221574
10.3390/microorganisms9051024
10.3390/microorganisms8060889
10.1016/j.biortech.2015.01.014
10.1093/bioinformatics/btu033
10.3390/microorganisms8101557
10.5194/gmd-5-1471-2012
10.1099/ijsem.0.000760
10.1186/s40168-018-0607-0
10.1093/bioinformatics/bty266
10.1089/cmb.2012.0021
10.1093/nar/gkw569
10.1038/s41598-019-44124-3
10.1093/aob/mcm240
10.1111/1462-2920.13842
10.5962/bhl.title.84653
ContentType Journal Article
Copyright 2022 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.
2022 by the authors. 2022
Copyright_xml – notice: 2022 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.
– notice: 2022 by the authors. 2022
DBID AAYXX
CITATION
NPM
7QP
7TK
8FD
8FE
8FH
ABUWG
AFKRA
AZQEC
BBNVY
BENPR
BHPHI
CCPQU
DWQXO
FR3
GNUQQ
HCIFZ
LK8
M7P
P64
PHGZM
PHGZT
PIMPY
PKEHL
PQEST
PQGLB
PQQKQ
PQUKI
PRINS
RC3
7X8
5PM
DOA
DOI 10.3390/biology11040519
DatabaseName CrossRef
PubMed
Calcium & Calcified Tissue Abstracts
Neurosciences Abstracts
Technology Research Database
ProQuest SciTech Collection
ProQuest Natural Science Collection
ProQuest Central (Alumni)
ProQuest Central UK/Ireland
ProQuest Central Essentials
Biological Science Collection
ProQuest Central
Natural Science Collection
ProQuest One Community College
ProQuest Central Korea
Engineering Research Database
ProQuest Central Student
SciTech Premium Collection
Biological Sciences
Biological Science Database
Biotechnology and BioEngineering Abstracts
ProQuest Central Premium
ProQuest One Academic
Publicly Available Content Database
ProQuest One Academic Middle East (New)
ProQuest One Academic Eastern Edition (DO NOT USE)
ProQuest One Applied & Life Sciences
ProQuest One Academic
ProQuest One Academic UKI Edition
ProQuest Central China
Genetics Abstracts
MEDLINE - Academic
PubMed Central (Full Participant titles)
Directory of Open Access Journals - May need to register for free articles
DatabaseTitle CrossRef
PubMed
Publicly Available Content Database
ProQuest Central Student
Technology Research Database
ProQuest One Academic Middle East (New)
ProQuest Central Essentials
ProQuest Central (Alumni Edition)
SciTech Premium Collection
ProQuest One Community College
ProQuest Natural Science Collection
ProQuest Central China
ProQuest Central
ProQuest One Applied & Life Sciences
Genetics Abstracts
Natural Science Collection
ProQuest Central Korea
Biological Science Collection
ProQuest Central (New)
ProQuest Biological Science Collection
ProQuest One Academic Eastern Edition
Biological Science Database
ProQuest SciTech Collection
Neurosciences Abstracts
Biotechnology and BioEngineering Abstracts
ProQuest One Academic UKI Edition
Engineering Research Database
ProQuest One Academic
Calcium & Calcified Tissue Abstracts
ProQuest One Academic (New)
MEDLINE - Academic
DatabaseTitleList
PubMed
Publicly Available Content Database
CrossRef
MEDLINE - Academic
Database_xml – sequence: 1
  dbid: DOA
  name: DOAJ Directory of Open Access Journals
  url: https://www.doaj.org/
  sourceTypes: Open Website
– sequence: 2
  dbid: NPM
  name: PubMed
  url: https://proxy.k.utb.cz/login?url=http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed
  sourceTypes: Index Database
– sequence: 3
  dbid: BENPR
  name: ProQuest Central
  url: https://www.proquest.com/central
  sourceTypes: Aggregation Database
DeliveryMethod fulltext_linktorsrc
Discipline Biology
EISSN 2079-7737
ExternalDocumentID oai_doaj_org_article_53b23ca5207f4814b3032843a31c9ad4
PMC9030188
35453719
10_3390_biology11040519
Genre Journal Article
GeographicLocations United Kingdom--UK
Thailand
United States--US
GeographicLocations_xml – name: United Kingdom--UK
– name: Thailand
– name: United States--US
GrantInformation_xml – fundername: European Research Council
  grantid: IsoMet 694578
– fundername: Biodiversity-based Economy Development Office (Public Organisation) (BEDO)
  grantid: 36/2562
– fundername: Natural Environment Research Council
  grantid: NE/J009555/1
GroupedDBID 2XV
53G
5VS
8FE
8FH
AADQD
AAFWJ
AAHBH
AAYXX
ADBBV
AFKRA
AFPKN
AFZYC
ALMA_UNASSIGNED_HOLDINGS
AOIJS
BBNVY
BCNDV
BENPR
BHPHI
CCPQU
CITATION
EBD
GROUPED_DOAJ
HCIFZ
HYE
IAO
IHR
ITC
KQ8
LK8
M48
M7P
MODMG
M~E
OK1
PGMZT
PHGZM
PHGZT
PIMPY
PROAC
RPM
ISR
NPM
7QP
7TK
8FD
ABUWG
AZQEC
DWQXO
FR3
GNUQQ
P64
PKEHL
PQEST
PQGLB
PQQKQ
PQUKI
PRINS
RC3
7X8
5PM
PUEGO
ID FETCH-LOGICAL-c487t-1e750d04a0f06925cf6a1b71eb02004f5885ad96061fb9e1b7ac0ba451e210133
IEDL.DBID M48
ISSN 2079-7737
IngestDate Wed Aug 27 01:15:33 EDT 2025
Thu Aug 21 14:03:31 EDT 2025
Thu Jul 10 18:09:48 EDT 2025
Fri Jul 25 10:44:22 EDT 2025
Thu Jan 02 22:52:55 EST 2025
Thu Apr 24 22:58:19 EDT 2025
Tue Jul 01 01:28:46 EDT 2025
IsDoiOpenAccess true
IsOpenAccess true
IsPeerReviewed true
IsScholarly true
Issue 4
Keywords genome
isoprene degradation
climate-active gas
isoprene
Alcaligenes
Language English
License https://creativecommons.org/licenses/by/4.0
Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
LinkModel DirectLink
MergedId FETCHMERGED-LOGICAL-c487t-1e750d04a0f06925cf6a1b71eb02004f5885ad96061fb9e1b7ac0ba451e210133
Notes ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 14
content type line 23
ORCID 0000-0002-3355-0071
0000-0002-1497-8822
OpenAccessLink http://journals.scholarsportal.info/openUrl.xqy?doi=10.3390/biology11040519
PMID 35453719
PQID 2652958834
PQPubID 2032427
ParticipantIDs doaj_primary_oai_doaj_org_article_53b23ca5207f4814b3032843a31c9ad4
pubmedcentral_primary_oai_pubmedcentral_nih_gov_9030188
proquest_miscellaneous_2654298673
proquest_journals_2652958834
pubmed_primary_35453719
crossref_primary_10_3390_biology11040519
crossref_citationtrail_10_3390_biology11040519
ProviderPackageCode CITATION
AAYXX
PublicationCentury 2000
PublicationDate 20220328
PublicationDateYYYYMMDD 2022-03-28
PublicationDate_xml – month: 3
  year: 2022
  text: 20220328
  day: 28
PublicationDecade 2020
PublicationPlace Switzerland
PublicationPlace_xml – name: Switzerland
– name: Basel
PublicationTitle Biology (Basel, Switzerland)
PublicationTitleAlternate Biology (Basel)
PublicationYear 2022
Publisher MDPI AG
MDPI
Publisher_xml – name: MDPI AG
– name: MDPI
References Gray (ref_9) 2015; 3
Srivastva (ref_50) 2017; 242
Tan (ref_25) 2005; 55
Cleveland (ref_7) 1997; 24
ref_51
Hadar (ref_54) 2019; 9
ref_19
Guenther (ref_4) 2012; 5
ref_18
ref_17
Letunic (ref_44) 2019; 47
Griffiths (ref_35) 2000; 66
Hall (ref_37) 1999; 41
Goris (ref_52) 2007; 57
Kingma (ref_22) 1999; 181
Abbas (ref_26) 2015; 108
Bolger (ref_39) 2014; 30
Kingma (ref_21) 1998; 64
ref_28
ref_27
McGenity (ref_6) 2018; 12
Stavrakou (ref_1) 2008; 8
Rehfuss (ref_29) 2005; 28
Heine (ref_56) 2018; 84
Pacifico (ref_5) 2009; 43
Johnston (ref_15) 2017; 19
Altschul (ref_49) 1990; 215
Pan (ref_30) 2016; 6
Pegoraro (ref_12) 2006; 12
Wattam (ref_41) 2014; 42
Srivastva (ref_32) 2015; 188
ref_34
Exton (ref_13) 2009; 11
Cleveland (ref_10) 1998; 64
Gibson (ref_23) 2018; 6
Joo (ref_24) 2006; 40
Sharkey (ref_2) 2008; 101
Tatusova (ref_48) 2016; 44
ref_38
Panke (ref_57) 1998; 64
Mikheenko (ref_46) 2018; 34
Arneth (ref_3) 2008; 8
Ewers (ref_31) 1990; 154
Crombie (ref_20) 2015; 17
McClay (ref_58) 2000; 66
Murrell (ref_8) 2020; 166
Weisburg (ref_36) 1991; 173
Elliott (ref_33) 2003; 184
Nakamura (ref_42) 2018; 34
Bankevich (ref_40) 2012; 19
Kumar (ref_55) 2018; 35
Leemhuis (ref_16) 2000; 182
Pegoraro (ref_11) 2005; 11
Crombie (ref_14) 2016; 18
Lee (ref_45) 2016; 66
Stamatakis (ref_43) 2014; 30
Manni (ref_47) 2021; 38
Konstantinidis (ref_53) 2005; 102
References_xml – volume: 66
  start-page: 1877
  year: 2000
  ident: ref_58
  article-title: Toluene monooxygenase-catalyzed epoxidation of alkenes
  publication-title: Appl. Environ. Microbiol.
  doi: 10.1128/AEM.66.5.1877-1882.2000
– volume: 181
  start-page: 2094
  year: 1999
  ident: ref_22
  article-title: Purification of a glutathione S-transferase and a glutathione conjugate-specific dehydrogenase involved in isoprene metabolism in Rhodococcus sp. strain AD45
  publication-title: J. Bacteriol.
  doi: 10.1128/JB.181.7.2094-2101.1999
– volume: 102
  start-page: 2567
  year: 2005
  ident: ref_53
  article-title: Genomic insights that advance the species definition for prokaryotes
  publication-title: Proc. Natl. Acad. Sci. USA
  doi: 10.1073/pnas.0409727102
– volume: 184
  start-page: 177
  year: 2003
  ident: ref_33
  article-title: Selecting framework tree species for restoring seasonally dry tropical forests in northern Thailand based on field performance
  publication-title: For. Ecol. Manag.
  doi: 10.1016/S0378-1127(03)00211-1
– volume: 38
  start-page: 4647
  year: 2021
  ident: ref_47
  article-title: BUSCO update: Novel and streamlined workflows along with broader and deeper phylogenetic coverage for scoring of eukaryotic, prokaryotic, and viral genomes
  publication-title: Mol. Biol. Evol.
  doi: 10.1093/molbev/msab199
– volume: 12
  start-page: 931
  year: 2018
  ident: ref_6
  article-title: Microbial cycling of Isoprene, the most abundantly produced biological volatile organic compound on earth
  publication-title: ISME J.
  doi: 10.1038/s41396-018-0072-6
– ident: ref_51
  doi: 10.3390/genes11020139
– volume: 3
  start-page: 000053
  year: 2015
  ident: ref_9
  article-title: Bacteria and fungi associated with isoprene consumption in soil
  publication-title: Elem. Sci. Anth.
  doi: 10.12952/journal.elementa.000053
– volume: 34
  start-page: 2490
  year: 2018
  ident: ref_42
  article-title: Parallelization of MAFFT for large-scale multiple sequence alignments
  publication-title: Bioinformatics
  doi: 10.1093/bioinformatics/bty121
– volume: 182
  start-page: 1956
  year: 2000
  ident: ref_16
  article-title: Characterization of the gene cluster involved in isoprene metabolism in Rhodococcus sp. strain AD45
  publication-title: J. Bacteriol.
  doi: 10.1128/JB.182.7.1956-1963.2000
– volume: 35
  start-page: 1547
  year: 2018
  ident: ref_55
  article-title: MEGA X: Molecular evolutionary genetics analysis across computing platforms
  publication-title: Mol. Biol. Evol.
  doi: 10.1093/molbev/msy096
– volume: 42
  start-page: D581
  year: 2014
  ident: ref_41
  article-title: PATRIC, the bacterial bioinformatics database and analysis resource
  publication-title: Nucleic Acids Res.
  doi: 10.1093/nar/gkt1099
– volume: 43
  start-page: 6121
  year: 2009
  ident: ref_5
  article-title: Isoprene emissions and climate
  publication-title: Atmos. Environ.
  doi: 10.1016/j.atmosenv.2009.09.002
– volume: 166
  start-page: 600
  year: 2020
  ident: ref_8
  article-title: Microbial metabolism of isoprene: A much-neglected climate-active gas
  publication-title: Microbiology
  doi: 10.1099/mic.0.000931
– volume: 28
  start-page: 421
  year: 2005
  ident: ref_29
  article-title: Alcaligenes faecalis subsp. phenolicus subsp. nov. a phenol-degrading, denitrifying bacterium isolated from a graywater bioprocessor
  publication-title: Syst. Appl. Microbiol.
  doi: 10.1016/j.syapm.2005.03.003
– volume: 11
  start-page: 1234
  year: 2005
  ident: ref_11
  article-title: The effect of elevated atmospheric CO2 and drought on sources and sinks of isoprene in a temperate and tropical rainforest mesocosm
  publication-title: Glob. Change Biol.
  doi: 10.1111/j.1365-2486.2005.00986.x
– volume: 108
  start-page: 859
  year: 2015
  ident: ref_26
  article-title: A heavy-metal tolerant novel bacterium, Alcaligenes pakistanensis sp. nov., isolated from industrial effluent in Pakistan
  publication-title: Antonie Van Leeuwenhoek
  doi: 10.1007/s10482-015-0540-1
– volume: 40
  start-page: 3029
  year: 2006
  ident: ref_24
  article-title: Piggery wastewater treatment using Alcaligenes Faecalis strain No. 4 with heterotrophic nitrification and aerobic denitrification
  publication-title: Water Res.
  doi: 10.1016/j.watres.2006.06.021
– volume: 173
  start-page: 697
  year: 1991
  ident: ref_36
  article-title: 16S ribosomal DNA amplification for phylogenetic study
  publication-title: J. Bacteriol.
  doi: 10.1128/jb.173.2.697-703.1991
– volume: 64
  start-page: 2032
  year: 1998
  ident: ref_57
  article-title: Towards a biocatalyst for (S)-styrene oxide production: Characterization of the styrene degradation pathway of Pseudomonas sp. strain VLB120
  publication-title: Appl. Environ. Microbiol.
  doi: 10.1128/AEM.64.6.2032-2043.1998
– volume: 64
  start-page: 172
  year: 1998
  ident: ref_10
  article-title: Microbial consumption of atmospheric isoprene in a temperate forest soil
  publication-title: Appl. Environ. Microbiol.
  doi: 10.1128/AEM.64.1.172-177.1998
– volume: 8
  start-page: 4605
  year: 2008
  ident: ref_3
  article-title: Why are estimates of global terrestrial isoprene emissions so similar (and why is this not so for monoterpenes)?
  publication-title: Atmos. Chem. Phys.
  doi: 10.5194/acp-8-4605-2008
– volume: 6
  start-page: 21332
  year: 2016
  ident: ref_30
  article-title: Biodegradation of DDT by Stenotrophomonas sp. DDT-1: Characterization and genome functional analysis
  publication-title: Sci. Rep.
  doi: 10.1038/srep21332
– volume: 8
  start-page: 1329
  year: 2008
  ident: ref_1
  article-title: Global isoprene emissions estimated using MEGAN, ECMWF analyses and a detailed canopy environment model
  publication-title: Atmos. Chem. Phys.
  doi: 10.5194/acp-8-1329-2008
– volume: 24
  start-page: 2379
  year: 1997
  ident: ref_7
  article-title: Consumption of atmospheric isoprene in soil
  publication-title: Geophys. Res. Lett.
  doi: 10.1029/97GL02451
– volume: 154
  start-page: 410
  year: 1990
  ident: ref_31
  article-title: Selection of trichloroethene (TCE) degrading bacteria that resist inactivation by TCE
  publication-title: Arch. Microbiol.
  doi: 10.1007/BF00276540
– volume: 30
  start-page: 2114
  year: 2014
  ident: ref_39
  article-title: Trimmomatic: A flexible trimmer for Illumina sequence data
  publication-title: Bioinformatics
  doi: 10.1093/bioinformatics/btu170
– ident: ref_38
– volume: 215
  start-page: 403
  year: 1990
  ident: ref_49
  article-title: Basic Local Alignment Search Tool
  publication-title: J. Mol. Biol.
  doi: 10.1016/S0022-2836(05)80360-2
– volume: 18
  start-page: 2743
  year: 2016
  ident: ref_14
  article-title: Isolation of isoprene degrading bacteria from soils, development of IsoA gene probes and identification of the active isoprene-degrading soil community using DNA-stable isotope probing: Isoprene-degrading bacteria
  publication-title: Env. Microbiol.
  doi: 10.1111/1462-2920.13345
– volume: 57
  start-page: 81
  year: 2007
  ident: ref_52
  article-title: DNA–DNA hybridization values and their relationship to whole-genome sequence similarities
  publication-title: Int. J. Syst. Evol. Microbiol.
  doi: 10.1099/ijs.0.64483-0
– volume: 41
  start-page: 95
  year: 1999
  ident: ref_37
  article-title: BioEdit: A user-friendly biological sequence alignment editor and analysis program for Window 95/98/NT
  publication-title: Nucl. Acids. Symp. Ser.
– volume: 47
  start-page: W256
  year: 2019
  ident: ref_44
  article-title: Interactive tree of life (ITOL) v4: Recent updates and new developments
  publication-title: Nucleic Acids Res.
  doi: 10.1093/nar/gkz239
– ident: ref_18
  doi: 10.3390/microorganisms8030349
– volume: 55
  start-page: 2571
  year: 2005
  ident: ref_25
  article-title: Alcaligenes aquatilis sp. nov., a novel bacterium from sediments of the Weser Estuary, Germany, and a salt marsh on Shem Creek in Charleston Harbor, USA
  publication-title: Int. J. Syst. Evol. Microbiol.
  doi: 10.1099/ijs.0.63849-0
– volume: 11
  start-page: 3280
  year: 2009
  ident: ref_13
  article-title: Characterization of marine isoprene-degrading communities
  publication-title: Environ. Microbiol.
  doi: 10.1111/j.1462-2920.2009.02069.x
– volume: 242
  start-page: 87
  year: 2017
  ident: ref_50
  article-title: Kinetic and molecular analyses reveal isoprene degradation potential of Methylobacterium sp.
  publication-title: Bioresour. Technol.
  doi: 10.1016/j.biortech.2017.02.002
– volume: 64
  start-page: 2800
  year: 1998
  ident: ref_21
  article-title: A glutathione S-transferase with activity towards cis-1,2-dichloroepoxyethane is involved in isoprene utilization by Rhodococcus sp. strain AD45
  publication-title: Appl. Environ. Microbiol.
  doi: 10.1128/AEM.64.8.2800-2805.1998
– volume: 17
  start-page: 3314
  year: 2015
  ident: ref_20
  article-title: Regulation of plasmid-encoded isoprene metabolism in Rhodococcus, a representative of an important link in the global isoprene cycle
  publication-title: Env. Microbiol.
  doi: 10.1111/1462-2920.12793
– volume: 84
  start-page: e00154-18
  year: 2018
  ident: ref_56
  article-title: On the enigma of glutathione-dependent styrene degradation in Gordonia rubripertincta CWB2
  publication-title: Appl. Environ. Microbiol.
  doi: 10.1128/AEM.00154-18
– volume: 12
  start-page: 456
  year: 2006
  ident: ref_12
  article-title: Drought effect on isoprene production and consumption in biosphere 2 tropical rainforest
  publication-title: Glob. Chang. Biol.
  doi: 10.1111/j.1365-2486.2006.01112.x
– volume: 66
  start-page: 5488
  year: 2000
  ident: ref_35
  article-title: Rapid method for coextraction of DNA and RNA from natural environments for analysis of ribosomal DNA- and rRNA-based microbial community composition
  publication-title: Appl. Environ. Microbiol.
  doi: 10.1128/AEM.66.12.5488-5491.2000
– ident: ref_27
  doi: 10.1371/journal.pone.0221574
– ident: ref_34
  doi: 10.3390/microorganisms9051024
– ident: ref_17
  doi: 10.3390/microorganisms8060889
– volume: 188
  start-page: 84
  year: 2015
  ident: ref_32
  article-title: Characterization of bacterial isolates from rubber dump site and their use in biodegradation of isoprene in batch and continuous bioreactors
  publication-title: Bioresour. Technol.
  doi: 10.1016/j.biortech.2015.01.014
– volume: 30
  start-page: 1312
  year: 2014
  ident: ref_43
  article-title: RAxML version 8: A tool for phylogenetic analysis and post-analysis of large phylogenies
  publication-title: Bioinformatics
  doi: 10.1093/bioinformatics/btu033
– ident: ref_19
  doi: 10.3390/microorganisms8101557
– volume: 5
  start-page: 1471
  year: 2012
  ident: ref_4
  article-title: The model of emissions of gases and aerosols from Nature version 2.1 (MEGAN2.1): An extended and updated framework for modeling biogenic emissions
  publication-title: Geosci. Model Dev.
  doi: 10.5194/gmd-5-1471-2012
– volume: 66
  start-page: 1100
  year: 2016
  ident: ref_45
  article-title: OrthoANI: An improved algorithm and software for calculating average nucleotide identity
  publication-title: Int. J. Syst. Evol. Microbiol.
  doi: 10.1099/ijsem.0.000760
– volume: 6
  start-page: 219
  year: 2018
  ident: ref_23
  article-title: Gene probing reveals the widespread distribution, diversity and abundance of isoprene-degrading bacteria in the environment
  publication-title: Microbiome
  doi: 10.1186/s40168-018-0607-0
– volume: 34
  start-page: i142
  year: 2018
  ident: ref_46
  article-title: Versatile genome assembly evaluation with QUAST-LG
  publication-title: Bioinformatics
  doi: 10.1093/bioinformatics/bty266
– volume: 19
  start-page: 455
  year: 2012
  ident: ref_40
  article-title: SPAdes: A New Genome Assembly Algorithm and Its Applications to Single-Cell Sequencing
  publication-title: J. Comput. Biol.
  doi: 10.1089/cmb.2012.0021
– volume: 44
  start-page: 6614
  year: 2016
  ident: ref_48
  article-title: NCBI Prokaryotic Genome Annotation Pipeline
  publication-title: Nucleic Acids Res.
  doi: 10.1093/nar/gkw569
– volume: 9
  start-page: 7613
  year: 2019
  ident: ref_54
  article-title: Altering N2O emissions by manipulating wheat root bacterial community
  publication-title: Sci. Rep.
  doi: 10.1038/s41598-019-44124-3
– volume: 101
  start-page: 5
  year: 2008
  ident: ref_2
  article-title: isoprene emission from plants: Why and how
  publication-title: Ann. Bot.
  doi: 10.1093/aob/mcm240
– volume: 19
  start-page: 3526
  year: 2017
  ident: ref_15
  article-title: Identification and characterisation of isoprene-degrading bacteria in an estuarine environment
  publication-title: Env. Microbiol.
  doi: 10.1111/1462-2920.13842
– ident: ref_28
  doi: 10.5962/bhl.title.84653
SSID ssj0000702636
Score 2.2832146
Snippet Isoprene is a climate-active biogenic volatile organic compound (BVOC), emitted into the atmosphere in abundance, mainly from terrestrial plants. Soil is an...
Simple SummaryIsoprene, a volatile hydrocarbon, is the second most abundantly produced climate-active gas, with largely indirect detrimental impacts, such as...
SourceID doaj
pubmedcentral
proquest
pubmed
crossref
SourceType Open Website
Open Access Repository
Aggregation Database
Index Database
Enrichment Source
StartPage 519
SubjectTerms Alcaligenes
Bacteria
Biodegradation
Carbon
climate-active gas
Enzymes
Gas chromatography
genome
Genomes
Genomic analysis
Gram-positive bacteria
Greenhouse gases
Isoprene
isoprene degradation
Metabolic pathways
Metabolism
Monooxygenase
National parks
Organic compounds
Prunus cerasoides
Soil microorganisms
VOCs
Volatile organic compounds
SummonAdditionalLinks – databaseName: Directory of Open Access Journals - May need to register for free articles
  dbid: DOA
  link: http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwrV1LS8QwEA4iCF7Et6urRPDgpZombZMefT9AD7KCt5K0CQpru2x3D_57Z9K67i6KF6-dKUwzM535SPINIUec56aAShbkykgAKFoEJhRFwK00xjomnCeef3hMbp-j-5f4ZWrUF54Ja-iBm4U7jYXhItcxZ9JFKoyMQAa4SGgR5qkuPBMo1LwpMOX_wRKwBe5LIpePAFx_2nIaQbWLsGuZKUOerf-nFnP-pORU6bleJSttz0jPGlvXyIIt18lSM0XyY4O83diyerf04pt72S83rRzVJb2rK89bGVwiLwSWKnrWB88gDaetaT04QZU-9JwFxcsmVNPesBqg8-iTnzsDApzgWY82yfP1Ve_iNmgnKAQ5AJFREFpoCAoWaeZYkvI4d4kOjQytYZgdLlYq1gWCmNCZ1IJI58zoKA4tQEGAr1tksaxKu0OoAr-luG2pZAErmGqccGRikzJnoEdiHXLytaBZ3tKL45SLfgYwAz2QzXmgQ44nLwwaZo3fVc_RQxM1pMT2DyBQsjZQsr8CpUO6X_7N2jytM57gRqdSAsSHEzFkGG6b6NJWY68DRVslUnTIdhMOE0sENKBCooVyJlBmTJ2VlG-vnsU7RTCq1O5_fNseWeZ4LYOJgKsuWRwNx3YfmqWROfB58QmnMhGO
  priority: 102
  providerName: Directory of Open Access Journals
– databaseName: ProQuest Central
  dbid: BENPR
  link: http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwfV1Lb9QwEB7BVkhcEO8GWmQkDlzcOnYezqlqS0tBokJVK_UW2YndVlqSZbM98O-Z8XpTtgKuGUcazcMz47G_AfggZWNbjGS80bbEAsUoblPVculKa50Xygfg-W-nxclF9vUyv4wHbkO8VrnaE8NG3fYNnZHvyoJaUlqrbG_2k9PUKOquxhEaD2EDt2CtJ7BxcHT6_Ww8ZUGDlgX1JwnTR2F9vxuxjTDqZZS9rIWjgNr_t1Tz_o3JP0LQ8VN4EnNHtr9U9jN44Lrn8Gg5TfLXC7j57Lr-h2OHdxjMQeys98x07MvQB_xK_onwIShksf0paojgON3AhtkOLZli7tkyenTCDDuf9zNSIjsL82eQQJM8h8VLuDg-Oj884XGSAm-wIFnw1GFi0IrMCC-KSuaNL0xqy9RZQV7iUa65aamYSb2tHJJMI6zJ8tRhSYhl7CuYdH3nNoFp1F9F7UtdtijBytCkI5vbSniLuZJIYGcl0LqJMOM07WJaY7lBGqjvaSCBj-MPsyXCxr-XHpCGxmUEjR0-9POrOnpanSsrVWNyKUqfaeRNEWRgpoxKm8q0WQJbK_3W0V-H-s66Eng_ktHTqH1iOtffhjUYvHVRqgReL81h5ERhIqpK4rBcM5Q1Vtcp3c11QPOuqCjV-s3_2XoLjyU9vBCKS70Fk8X81m1jOrSw76LN_wYyMAu-
  priority: 102
  providerName: ProQuest
Title Genome Characterisation of an Isoprene-Degrading Alcaligenes sp. Isolated from a Tropical Restored Forest
URI https://www.ncbi.nlm.nih.gov/pubmed/35453719
https://www.proquest.com/docview/2652958834
https://www.proquest.com/docview/2654298673
https://pubmed.ncbi.nlm.nih.gov/PMC9030188
https://doaj.org/article/53b23ca5207f4814b3032843a31c9ad4
Volume 11
hasFullText 1
inHoldings 1
isFullTextHit
isPrint
link http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwrV1NT9wwEB21oEq9oH5Bl9KVK_XQSxbHTmLnUFV8FpBAFWIlbpGd2C3SNlk2iwT_vjPe7NJFcOO640iOx5N5b528B_BViNJW2MmiUluFBMXIyMayioRT1jrPpQ_C86dn2dEwOblML-_tgLoFbB-lduQnNZyMBrfXdz-w4L8T40TKvt3JFWEjSwiQvIRVbEuK7AxOO6wfHssK6QYdVW48cd1SZwoC_o-hzocvT_7XjQ7fwFoHI9nOLO9v4YWr38GrmbHk3Xu4-unq5q9je_dyzCEDrPHM1Oy4bYKUZbRPUhHUvdjOCJNFypyuZe14QENGCEMrRt-fMMMuJs2Y8snOgxUNBsjUs51-gOHhwcXeUdSZKkQlcpNpFDvECBVPDPc8y0Va-szEVsXOcioYn2qdmop4Text7jBkSm5NksYO2SEy2nVYqZvafQSmMZU5nWRqVeEK5oZMj2xqc-4twibeg8F8QYuyUxwn44tRgcyDMlA8yEAPvi0uGM_ENp4euksZWgwjlezwQzP5XXRFV6TSClmaVHDlE41zk6QemEgj4zI3VdKDrXl-i_nOK0RGZ59aSwx_WYSx6OgkxdSuuQljsI_rTMkebMy2w2ImEjGpVDRDtbRRlqa6HKmv_gRh75z4qdabz3Fvn-C1oC81uIyE3oKV6eTGfUb8NLV9WN09OPt13g__P_RDlfwDDucdJA
linkProvider Scholars Portal
linkToHtml http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwtV1Lb9QwEB5VWyG4VLwJtGAkkLikdew8nAOq-mSXtitUbaXegp04pdKSLJutUP8Uv5GZvMpWwK3X2FFGns-ez574G4B3QqQmw0jmpspEuEHR0jWezFxhI2NszmVeC8-fjMPhmf_5PDhfgV_dXRj6rbJbE-uFOitTOiPfEiGlpJSS_vbsh0tVoyi72pXQaGBxZK9_4pat-jjaR_--F-LwYLI3dNuqAm6K5HzhehaDZMZ9zXMexiJI81B7JvKs4YSYHL8R6IyIvZeb2GKTTrnRfuBZ3B55dACKS_6qL0MuBrC6ezD-ctqf6uAEEiHlQ0lDSMqYb7VaShhlfWJLS-GvrhLwN2p7-w_NP0Le4UNYa7kq22nA9QhWbPEY7jXVK6-fwOUnW5TfLdu70Xyu3czKnOmCjaqy1st090mPgkIk25kiIkj-01asmm1Slyly3YzRJRem2WRezgg07LSud4MNVDm0WjyFszsZ42cwKMrCvgCmEC8xpUtVlOEIxpoqK5nAxDw3yM24A5vdgCZpK2tO1TWmCW5vyAPJLQ848KF_YdYoevy76y55qO9GUtz1g3J-kbQzOwmkETLVgeBR7iu0TZJEoS-19NJYZ74D651_k3Z9qJIbNDvwtm_GmU3pGl3Y8qrug2RBhZF04HkDh94SicRXRmRhtASUJVOXW4rLb7V6eEybYKVe_t-sN3B_ODk5To5H46NX8EDQpQ8uXaHWYbCYX9kNpGIL87rFP4Ovdz3lfgOo9kdT
linkToPdf http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwtV1LbxMxEB5VqUBcEG-2FDASSFy28dr7PCDUNg0NhaiqWqm3rb1rQ6V0N2RTof41fh0z-yqpgFuvsVcZeb7xzHjsbwDeCpHpHD2Zm8U6wgRFSVd7MneFibQ2lktbE89_nYb7J_7n0-B0DX51b2HoWmW3J9YbdV5mdEY-FCGVpOJY-kPbXos4HI0_zn-41EGKKq1dO40GIgfm6iemb9WHyQh1_U6I8d7x7r7bdhhwMwzUl65n0GHm3Ffc8jARQWZD5enIM5oTeiz-X6ByCvI9qxODQyrjWvmBZzBV8ugwFLf_9YiyogGs7-xND4_6Ex40JhFSbZT4hKRM-LDlVUKP61PktOIK644Bfwtzb97W_MP9jR_A_TZuZdsN0B7CmikewZ2mk-XVYzj_ZIrywrDda_7nWuWstEwVbFKVNXemOyJuCnKXbHuG6CAqUFOxar5FU2YY9-aMHrwwxY4X5ZwAxI7q3jc4QF1Eq-UTOLmVNX4Kg6IszHNgMWInodJpHOW4gomiLks60Am3GuM07sBWt6Bp1lKcU6eNWYqpDmkgvaEBB973H8wbdo9_T90hDfXTiJa7_qFcfEtbK08DqYXMVCB4ZP0YZZNEV-hLJb0sUbnvwGan37TdK6r0GtkOvOmH0cqpdKMKU17WczBwiMNIOvCsgUMvicQgWEYkYbQClBVRV0eK8-81k3hCCXEcb_xfrNdwF00t_TKZHryAe4Lef3DpingTBsvFpXmJUdlSv2rhz-Dsti3uN9S0S4g
openUrl ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fsummon.serialssolutions.com&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Ajournal&rft.genre=article&rft.atitle=Genome+Characterisation+of+an+Isoprene-Degrading+Alcaligenes+sp.+Isolated+from+a+Tropical+Restored+Forest&rft.jtitle=Biology+%28Basel%2C+Switzerland%29&rft.au=Toungporn+Uttarotai&rft.au=Sawannee+Sutheeworapong&rft.au=Andrew+T.+Crombie&rft.au=J.+Colin+Murrell&rft.date=2022-03-28&rft.pub=MDPI+AG&rft.eissn=2079-7737&rft.volume=11&rft.issue=4&rft.spage=519&rft_id=info:doi/10.3390%2Fbiology11040519&rft.externalDBID=DOA&rft.externalDocID=oai_doaj_org_article_53b23ca5207f4814b3032843a31c9ad4
thumbnail_l http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/lc.gif&issn=2079-7737&client=summon
thumbnail_m http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/mc.gif&issn=2079-7737&client=summon
thumbnail_s http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/sc.gif&issn=2079-7737&client=summon