Ablation of Perlecan Domain 1 Heparan Sulfate Reduces Progressive Cartilage Degradation, Synovitis, and Osteophyte Size in a Preclinical Model of Posttraumatic Osteoarthritis

• Published in: Arthritis & rheumatology (Hoboken, N.J.) Vol. 68; no. 4; pp. 868 - 879
• Main Authors: Shu, Cindy C., Jackson, Miriam T., Smith, Margaret M., Smith, Susan M., Penm, Steven, Lord, Megan S., Whitelock, John M., Little, Christopher B., Melrose, James
• Format: Journal Article
• Language: English
• Published: United States Wiley Subscription Services, Inc 01.04.2016
• Subjects: Animals; Blotting, Western; Cartilage, Articular - metabolism; Cartilage, Articular - pathology; Disease Progression; Fibroblast Growth Factor 2 - metabolism; Fibroblast Growth Factors - metabolism; Gene Expression Profiling; Glycosaminoglycans - metabolism; Heparan sulfate; Heparan Sulfate Proteoglycans - genetics; Heparan Sulfate Proteoglycans - metabolism; Immunohistochemistry; Knee Injuries - complications; Matrix Metalloproteinase 2 - genetics; Matrix Metalloproteinase 3 - genetics; Mice; Mice, Knockout; Organ Size; Osteoarthritis, Knee - etiology; Osteoarthritis, Knee - genetics; Osteoarthritis, Knee - pathology; Osteophyte - etiology; Osteophyte - genetics; Osteophyte - pathology; Osteosclerosis - etiology; Osteosclerosis - genetics; Osteosclerosis - pathology; Receptor, Fibroblast Growth Factor, Type 1 - metabolism; Receptor, Fibroblast Growth Factor, Type 3 - genetics; Receptor, Fibroblast Growth Factor, Type 3 - metabolism; RNA, Messenger - metabolism; Rodents; Synovitis - etiology; Synovitis - genetics; Synovitis - pathology

Objective To investigate the role of the heparan sulfate (HS) proteoglycan perlecan (HSPG‐2) in regulating fibroblast growth factor (FGF) activity, bone and joint growth, and the onset and progression of posttraumatic osteoarthritis (OA) in a mouse gene‐knockout model. Methods Maturational changes w...