miR‐126‐3p Promotes Matrix‐Dependent Perivascular Cell Attachment, Migration and Intercellular Interaction

microRNAs (miRNAs) can regulate the interplay between perivascular cells (PVC) and endothelial cells (EC) during angiogenesis, but the relevant PVC‐specific miRNAs are not yet defined. Here, we identified miR‐126‐3p and miR‐146a to be exclusively upregulated in PVC upon interaction with EC, determin...

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Published inStem cells (Dayton, Ohio) Vol. 34; no. 5; pp. 1297 - 1309
Main Authors Pitzler, Lena, Auler, Markus, Probst, Kristina, Frie, Christian, Bergmeier, Vera, Holzer, Tatjana, Belluoccio, Daniele, van den Bergen, Jocelyn, Etich, Julia, Ehlen, Harald, Zhou, Zhigang, Bielke, Wolfgang, Pöschl, Ernst, Paulsson, Mats, Brachvogel, Bent
Format Journal Article
LanguageEnglish
Published United States Oxford University Press 01.05.2016
Subjects
Angiogenesis
Animals
Blood Vessels - cytology
Cell Adhesion - drug effects
Cell Adhesion - genetics
Cell Communication - drug effects
Cell Communication - genetics
Cell Movement - drug effects
Cell Movement - genetics
Cell Shape - drug effects
Chemokines - metabolism
Coculture Techniques
Collagen - pharmacology
Drug Combinations
Extracellular Matrix - drug effects
Extracellular Matrix - metabolism
Extracellular Signal-Regulated MAP Kinases - metabolism
Gene Silencing - drug effects
Human Umbilical Vein Endothelial Cells - cytology
Human Umbilical Vein Endothelial Cells - drug effects
Human Umbilical Vein Endothelial Cells - metabolism
Humans
Laminin - pharmacology
MAP Kinase Signaling System - drug effects
Mice
MicroRNAs - genetics
MicroRNAs - metabolism
miRNA
miR‐126‐3p
Neovascularization, Physiologic - genetics
Perivascular cells
Plk2
Proteoglycans - pharmacology
Spred1
Tlr3
Transcriptome - genetics
Up-Regulation - drug effects
Up-Regulation - genetics
Angiogenesis
Perivascular cells
Plk2
miRNA
miR-126-3p
Spred1
Tlr3
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Abstract microRNAs (miRNAs) can regulate the interplay between perivascular cells (PVC) and endothelial cells (EC) during angiogenesis, but the relevant PVC‐specific miRNAs are not yet defined. Here, we identified miR‐126‐3p and miR‐146a to be exclusively upregulated in PVC upon interaction with EC, determined their influence on the PVC phenotype and elucidate their molecular mechanisms of action. Specifically the increase of miR‐126‐3p strongly promoted the motility of PVC on the basement membrane‐like composite and stabilized networks of EC. Subsequent miRNA target analysis showed that miR‐126‐3p inhibits SPRED1 and PLK2 expression, induces ERK1/2 phosphorylation and stimulates TLR3 expression to modulate cell‐cell and cell‐matrix contacts of PVC. Gain of expression experiments in vivo demonstrated that miR‐126‐3p stimulates PVC coverage of newly formed vessels and transform immature into mature, less permeable vessels. In conclusion we showed that miR‐126‐3p regulates matrix‐dependent PVC migration and intercellular interaction to modulate vascular integrity. Stem Cells 2016;34:1297–1309
AbstractList microRNAs (miRNAs) can regulate the interplay between perivascular cells (PVC) and endothelial cells (EC) during angiogenesis, but the relevant PVC-specific miRNAs are not yet defined. Here, we identified miR-126-3p and miR-146a to be exclusively upregulated in PVC upon interaction with EC, determined their influence on the PVC phenotype and elucidate their molecular mechanisms of action. Specifically the increase of miR-126-3p strongly promoted the motility of PVC on the basement membrane-like composite and stabilized networks of EC. Subsequent miRNA target analysis showed that miR-126-3p inhibits SPRED1 and PLK2 expression, induces ERK1/2 phosphorylation and stimulates TLR3 expression to modulate cell-cell and cell-matrix contacts of PVC. Gain of expression experiments in vivo demonstrated that miR-126-3p stimulates PVC coverage of newly formed vessels and transform immature into mature, less permeable vessels. In conclusion we showed that miR-126-3p regulates matrix-dependent PVC migration and intercellular interaction to modulate vascular integrity.
microRNAs (miRNAs) can regulate the interplay between perivascular cells (PVC) and endothelial cells (EC) during angiogenesis, but the relevant PVC-specific miRNAs are not yet defined. Here, we identified miR-126-3p and miR-146a to be exclusively upregulated in PVC upon interaction with EC, determined their influence on the PVC phenotype and elucidate their molecular mechanisms of action. Specifically the increase of miR-126-3p strongly promoted the motility of PVC on the basement membrane-like composite and stabilized networks of EC. Subsequent miRNA target analysis showed that miR-126-3p inhibits SPRED1 and PLK2 expression, induces ERK1/2 phosphorylation and stimulates TLR3 expression to modulate cell-cell and cell-matrix contacts of PVC. Gain of expression experiments in vivo demonstrated that miR-126-3p stimulates PVC coverage of newly formed vessels and transform immature into mature, less permeable vessels. In conclusion we showed that miR-126-3p regulates matrix-dependent PVC migration and intercellular interaction to modulate vascular integrity. Stem Cells 2016; 34:1297-1309
microRNAs (miRNAs) can regulate the interplay between perivascular cells (PVC) and endothelial cells (EC) during angiogenesis, but the relevant PVC-specific miRNAs are not yet defined. Here, we identified miR-126-3p and miR-146a to be exclusively upregulated in PVC upon interaction with EC, determined their influence on the PVC phenotype and elucidate their molecular mechanisms of action. Specifically the increase of miR-126-3p strongly promoted the motility of PVC on the basement membrane-like composite and stabilized networks of EC. Subsequent miRNA target analysis showed that miR-126-3p inhibits SPRED1 and PLK2 expression, induces ERK1/2 phosphorylation and stimulates TLR3 expression to modulate cell-cell and cell-matrix contacts of PVC. Gain of expression experiments in vivo demonstrated that miR-126-3p stimulates PVC coverage of newly formed vessels and transform immature into mature, less permeable vessels. In conclusion we showed that miR-126-3p regulates matrix-dependent PVC migration and intercellular interaction to modulate vascular integrity. Stem Cells 2016;34:1297-1309.
Author Paulsson, Mats
Frie, Christian
Probst, Kristina
Etich, Julia
Pöschl, Ernst
Auler, Markus
Bergmeier, Vera
Belluoccio, Daniele
Holzer, Tatjana
van den Bergen, Jocelyn
Brachvogel, Bent
Zhou, Zhigang
Ehlen, Harald
Pitzler, Lena
Bielke, Wolfgang
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Cites_doi 10.1016/j.neuron.2011.02.004
10.1016/j.ijcard.2013.01.160
10.1016/j.matbio.2015.02.014
10.1016/S0002-9440(10)64276-9
10.1038/35099560
10.1016/j.yexcr.2007.04.031
10.1016/j.yjmcc.2012.04.003
10.1002/stem.1629
10.1016/j.devcel.2008.07.008
10.1007/s10456-012-9285-x
10.1016/j.cell.2011.10.022
10.2202/1544-6115.1027
10.1042/bj2960297
10.1002/glia.20862
10.1016/0040-8166(86)90026-1
10.1038/nature10661
10.1093/nar/gkh029
10.1371/journal.pone.0051243
10.1096/fj.12-226977
10.1038/aps.2009.143
10.1093/nar/29.9.e45
10.1172/JCI23755
10.1152/physrev.00041.2003
10.1113/jphysiol.2011.208355
10.1002/cyto.1067
10.1038/ni.2767
10.1242/dev.01846
10.1016/0003-2697(87)90021-2
10.1016/j.molimm.2010.04.004
10.1126/science.1082475
10.1371/journal.pone.0053262
10.1074/jbc.M110.193086
10.1101/gad.190876.112
10.1161/CIRCRESAHA.113.280883
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Issue 5
Keywords Angiogenesis
Perivascular cells
Plk2
miRNA
miR-126-3p
Spred1
Tlr3
Language English
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References 1987; 162
2004; 84
2013; 69
2013; 27
2005; 132
2005; 115
2008; 15
2004; 3
1986; 18
2013; 168
2001; 29
2012; 15
2013; 8
2001; 46
2012; 53
2004; 32
2011; 589
2007; 313
2011; 147
2009; 57
2009; 30
2010; 47
2015; 43
2003; 162
2014; 15
2013; 113
2012; 26
2003; 302
2011; 481
1993; 296
2014; 32
2011; 286
2010; 8
2001; 413
28649792 - Stem Cells. 2017 Jul;35(7):1866
Brachvogel (2022010809592731700_stem2308-bib-0011) 2007; 313
Agudo (2022010809592731700_stem2308-bib-0033) 2014; 15
Kane (2022010809592731700_stem2308-bib-0004) 2014; 32
Rasband (2022010809592731700_stem2308-bib-0019)
Schaefer (2022010809592731700_stem2308-bib-0026) 2005; 115
Guedez (2022010809592731700_stem2308-bib-0020) 2003; 162
Lee (2022010809592731700_stem2308-bib-0030) 2013; 69
Smyth (2022010809592731700_stem2308-bib-0014) 2004; 3
Thuringer (2022010809592731700_stem2308-bib-0027) 2013; 27
Chevrier (2022010809592731700_stem2308-bib-0032) 2011; 147
Rosenbaum (2022010809592731700_stem2308-bib-0012) 2011; 286
Etich (2022010809592731700_stem2308-bib-0016) 2015; 43
Etich (2022010809592731700_stem2308-bib-0001) 2010; 8
Meng (2022010809592731700_stem2308-bib-0035) 2012; 53
Chow (2022010809592731700_stem2308-bib-0017) 2001; 46
Stowe (2022010809592731700_stem2308-bib-0029) 2012; 26
Gordeladze (2022010809592731700_stem2308-bib-0005) 2009; 30
Brachvogel (2022010809592731700_stem2308-bib-0010) 2005; 132
Peroval (2022010809592731700_stem2308-bib-0024) 2013; 8
Pfaffl (2022010809592731700_stem2308-bib-0015) 2001; 29
Fish (2022010809592731700_stem2308-bib-0006) 2008; 15
Png (2022010809592731700_stem2308-bib-0008) 2011; 481
Machado (2022010809592731700_stem2308-bib-0028) 2011; 589
Pak (2022010809592731700_stem2308-bib-0031) 2003; 302
Chomczynski (2022010809592731700_stem2308-bib-0013) 1987; 162
Visel (2022010809592731700_stem2308-bib-0022) 2004; 32
Coulpier (2022010809592731700_stem2308-bib-0021) 2009; 57
Qiang (2022010809592731700_stem2308-bib-0009) 2013; 168
Suffee (2022010809592731700_stem2308-bib-0023) 2012; 15
Alexopoulou (2022010809592731700_stem2308-bib-0034) 2001; 413
Mitchell (2022010809592731700_stem2308-bib-0025) 2010; 47
Owens (2022010809592731700_stem2308-bib-0003) 2004; 84
Zhou (2022010809592731700_stem2308-bib-0007) 2013; 113
Arcaro (2022010809592731700_stem2308-bib-0018) 1993; 296
Sims (2022010809592731700_stem2308-bib-0002) 1986; 18
References_xml – volume: 29
  start-page: e45
  year: 2001
  article-title: A new mathematical model for relative quantification in real‐time RT‐PCR
  publication-title: Nucleic Acids Res
– volume: 413
  start-page: 732
  year: 2001
  end-page: 738
  article-title: Recognition of double‐stranded RNA and activation of NF‐kappaB by Toll‐like receptor 3
  publication-title: Nature
– volume: 162
  start-page: 1431
  year: 2003
  end-page: 1439
  article-title: Quantitative assessment of angiogenic responses by the directed in vivo angiogenesis assay
  publication-title: Am J Pathol
– volume: 113
  start-page: 40
  year: 2013
  end-page: 51
  article-title: Regulation of vascular smooth muscle cell turnover by endothelial cell‐secreted MicroRNA‐126: Role of shear stress
  publication-title: Circ Res
– volume: 46
  start-page: 72
  year: 2001
  end-page: 78
  article-title: Measurement of MAP kinase activation by flow cytometry using phospho‐specific antibodies to MEK and ERK: Potential for pharmacodynamic monitoring of signal transduction inhibitors
  publication-title: Cytometry
– volume: 84
  start-page: 767
  year: 2004
  end-page: 801
  article-title: Molecular regulation of vascular smooth muscle cell differentiation in development and disease
  publication-title: Physiol Rev
– volume: 15
  start-page: 54
  year: 2014
  end-page: 62
  article-title: The miR‐126‐VEGFR2 axis controls the innate response to pathogen‐associated nucleic acids
  publication-title: Nat Immunol
– volume: 589
  start-page: 4681
  year: 2011
  end-page: 4696
  article-title: Temporal changes in microvessel leakiness during wound healing discriminated by in vivo fluorescence recovery after photobleaching
  publication-title: J Physiol
– volume: 43
  start-page: 27
  year: 2015
  end-page: 34
  article-title: MiR‐26a modulates extracellular matrix homeostasis in cartilage
  publication-title: Matrix Biol
– volume: 32
  start-page: D552
  year: 2004
  end-page: 556
  article-title: GenePaint.org: An atlas of gene expression patterns in the mouse embryo
  publication-title: Nucleic Acids Res
– volume: 15
  start-page: 727
  year: 2012
  end-page: 744
  article-title: RANTES/CCL5‐induced pro‐angiogenic effects depend on CCR1, CCR5 and glycosaminoglycans
  publication-title: Angiogenesis
– volume: 115
  start-page: 2223
  year: 2005
  end-page: 2233
  article-title: The matrix component biglycan is proinflammatory and signals through Toll‐like receptors 4 and 2 in macrophages
  publication-title: J Clin Invest
– volume: 15
  start-page: 272
  year: 2008
  end-page: 284
  article-title: miR‐126 regulates angiogenic signaling and vascular integrity
  publication-title: Dev Cell
– volume: 162
  start-page: 156
  year: 1987
  end-page: 159
  article-title: Single‐step method of RNA isolation by acid guanidinium thiocyanate‐phenol‐chloroform extraction
  publication-title: Anal Biochem
– volume: 47
  start-page: 2065
  year: 2010
  end-page: 2073
  article-title: Regulation of Toll‐like receptor‐induced chemokine production in murine dendritic cells by mitogen‐activated protein kinases
  publication-title: Mol Immunol
– volume: 286
  start-page: 5708
  year: 2011
  end-page: 5716
  article-title: Identification of novel binding partners (annexins) for the cell death signal phosphatidylserine and definition of their recognition motif
  publication-title: J Biol Chem
– volume: 69
  start-page: 957
  year: 2013
  end-page: 973
  article-title: Requirement for Plk2 in orchestrated ras and rap signaling, homeostatic structural plasticity, and memory
  publication-title: Neuron
– volume: 313
  start-page: 2730
  year: 2007
  end-page: 2743
  article-title: Isolated Anxa5(+)/Sca‐1(+) perivascular cells from mouse meningeal vasculature retain their perivascular phenotype in vitro and in vivo
  publication-title: Exp Cell Res
– volume: 8
  start-page: e51243
  year: 2013
  article-title: A critical role for MAPK signalling pathways in the transcriptional regulation of toll like receptors
  publication-title: PLoS One
– volume: 8
  start-page: e53262
  year: 2010
  article-title: PECAM1(+)/Sca1(+)/CD38(+) vascular cells transform into myofibroblast‐like cells in skin wound repair
  publication-title: PLoS ONE
– volume: 132
  start-page: 2657
  year: 2005
  end-page: 2668
  article-title: Perivascular cells expressing annexin A5 define a novel mesenchymal stem cell‐like population with the capacity to differentiate into multiple mesenchymal lineages
  publication-title: Development
– volume: 302
  start-page: 1368
  year: 2003
  end-page: 1373
  article-title: Targeted protein degradation and synapse remodeling by an inducible protein kinase
  publication-title: Science
– volume: 147
  start-page: 853
  year: 2011
  end-page: 867
  article-title: Systematic discovery of TLR signaling components delineates viral‐sensing circuits
  publication-title: Cell
– volume: 32
  start-page: 1059
  year: 2014
  end-page: 1066
  article-title: Concise review: MicroRNAs as modulators of stem cells and angiogenesis
  publication-title: Stem Cells
– volume: 18
  start-page: 153
  year: 1986
  end-page: 174
  article-title: The pericyte—A review
  publication-title: Tissue Cell
– volume: 481
  start-page: 190
  year: 2011
  end-page: 194
  article-title: A microRNA regulon that mediates endothelial recruitment and metastasis by cancer cells
  publication-title: Nature
– volume: 27
  start-page: 4169
  year: 2013
  end-page: 4183
  article-title: Extracellular HSP27 mediates angiogenesis through Toll‐like receptor 3
  publication-title: FASEB J
– volume: 3
  start-page: 1
  year: 2004
  end-page: 26
  article-title: Linear models and empirical bayes methods for assessing differential expression in microarray experiments
  publication-title: Stat Appl Genet Mol Biol
– volume: 296
  start-page: 297
  issue: Pt 2
  year: 1993
  end-page: 301
  article-title: Wortmannin is a potent phosphatidylinositol 3‐kinase inhibitor: The role of phosphatidylinositol 3,4,5‐trisphosphate in neutrophil responses
  publication-title: Biochem J
– volume: 57
  start-page: 1450
  year: 2009
  end-page: 1457
  article-title: Novel features of boundary cap cells revealed by the analysis of newly identified molecular markers
  publication-title: Glia
– volume: 53
  start-page: 64
  year: 2012
  end-page: 72
  article-title: Downregulation of microRNA‐126 in endothelial progenitor cells from diabetes patients, impairs their functional properties, via target gene Spred‐1
  publication-title: J Mol Cell Cardiol
– volume: 168
  start-page: 2082
  year: 2013
  end-page: 2088
  article-title: Expression of miR‐126 and miR‐508‐5p in endothelial progenitor cells is associated with the prognosis of chronic heart failure patients
  publication-title: Int J Cardiol
– volume: 26
  start-page: 1421
  year: 2012
  end-page: 1426
  article-title: A shared molecular mechanism underlies the human rasopathies Legius syndrome and Neurofibromatosis‐1
  publication-title: Genes Dev
– volume: 30
  start-page: 1369
  year: 2009
  end-page: 1384
  article-title: Concerted stimuli regulating osteo‐chondral differentiation from stem cells: Phenotype acquisition regulated by microRNAs
  publication-title: Acta Pharmacol Sin
– start-page: 1997
  end-page: 2011
– volume: 69
  start-page: 957
  year: 2013
  ident: 2022010809592731700_stem2308-bib-0030
  article-title: Requirement for Plk2 in orchestrated ras and rap signaling, homeostatic structural plasticity, and memory
  publication-title: Neuron
  doi: 10.1016/j.neuron.2011.02.004
  contributor:
    fullname: Lee
– volume: 168
  start-page: 2082
  year: 2013
  ident: 2022010809592731700_stem2308-bib-0009
  article-title: Expression of miR-126 and miR-508-5p in endothelial progenitor cells is associated with the prognosis of chronic heart failure patients
  publication-title: Int J Cardiol
  doi: 10.1016/j.ijcard.2013.01.160
  contributor:
    fullname: Qiang
– volume: 43
  start-page: 27
  year: 2015
  ident: 2022010809592731700_stem2308-bib-0016
  article-title: MiR-26a modulates extracellular matrix homeostasis in cartilage
  publication-title: Matrix Biol
  doi: 10.1016/j.matbio.2015.02.014
  contributor:
    fullname: Etich
– volume: 162
  start-page: 1431
  year: 2003
  ident: 2022010809592731700_stem2308-bib-0020
  article-title: Quantitative assessment of angiogenic responses by the directed in vivo angiogenesis assay
  publication-title: Am J Pathol
  doi: 10.1016/S0002-9440(10)64276-9
  contributor:
    fullname: Guedez
– volume: 413
  start-page: 732
  year: 2001
  ident: 2022010809592731700_stem2308-bib-0034
  article-title: Recognition of double-stranded RNA and activation of NF-kappaB by Toll-like receptor 3
  publication-title: Nature
  doi: 10.1038/35099560
  contributor:
    fullname: Alexopoulou
– volume: 313
  start-page: 2730
  year: 2007
  ident: 2022010809592731700_stem2308-bib-0011
  article-title: Isolated Anxa5(+)/Sca-1(+) perivascular cells from mouse meningeal vasculature retain their perivascular phenotype in vitro and in vivo
  publication-title: Exp Cell Res
  doi: 10.1016/j.yexcr.2007.04.031
  contributor:
    fullname: Brachvogel
– volume: 53
  start-page: 64
  year: 2012
  ident: 2022010809592731700_stem2308-bib-0035
  article-title: Downregulation of microRNA-126 in endothelial progenitor cells from diabetes patients, impairs their functional properties, via target gene Spred-1
  publication-title: J Mol Cell Cardiol
  doi: 10.1016/j.yjmcc.2012.04.003
  contributor:
    fullname: Meng
– volume: 32
  start-page: 1059
  year: 2014
  ident: 2022010809592731700_stem2308-bib-0004
  article-title: Concise review: MicroRNAs as modulators of stem cells and angiogenesis
  publication-title: Stem Cells
  doi: 10.1002/stem.1629
  contributor:
    fullname: Kane
– volume: 15
  start-page: 272
  year: 2008
  ident: 2022010809592731700_stem2308-bib-0006
  article-title: miR-126 regulates angiogenic signaling and vascular integrity
  publication-title: Dev Cell
  doi: 10.1016/j.devcel.2008.07.008
  contributor:
    fullname: Fish
– volume: 15
  start-page: 727
  year: 2012
  ident: 2022010809592731700_stem2308-bib-0023
  article-title: RANTES/CCL5-induced pro-angiogenic effects depend on CCR1, CCR5 and glycosaminoglycans
  publication-title: Angiogenesis
  doi: 10.1007/s10456-012-9285-x
  contributor:
    fullname: Suffee
– volume: 147
  start-page: 853
  year: 2011
  ident: 2022010809592731700_stem2308-bib-0032
  article-title: Systematic discovery of TLR signaling components delineates viral-sensing circuits
  publication-title: Cell
  doi: 10.1016/j.cell.2011.10.022
  contributor:
    fullname: Chevrier
– volume: 3
  start-page: 1
  year: 2004
  ident: 2022010809592731700_stem2308-bib-0014
  article-title: Linear models and empirical bayes methods for assessing differential expression in microarray experiments
  publication-title: Stat Appl Genet Mol Biol
  doi: 10.2202/1544-6115.1027
  contributor:
    fullname: Smyth
– volume: 296
  start-page: 297
  issue: Pt 2
  year: 1993
  ident: 2022010809592731700_stem2308-bib-0018
  article-title: Wortmannin is a potent phosphatidylinositol 3-kinase inhibitor: The role of phosphatidylinositol 3,4,5-trisphosphate in neutrophil responses
  publication-title: Biochem J
  doi: 10.1042/bj2960297
  contributor:
    fullname: Arcaro
– volume: 57
  start-page: 1450
  year: 2009
  ident: 2022010809592731700_stem2308-bib-0021
  article-title: Novel features of boundary cap cells revealed by the analysis of newly identified molecular markers
  publication-title: Glia
  doi: 10.1002/glia.20862
  contributor:
    fullname: Coulpier
– volume: 18
  start-page: 153
  year: 1986
  ident: 2022010809592731700_stem2308-bib-0002
  article-title: The pericyte—A review
  publication-title: Tissue Cell
  doi: 10.1016/0040-8166(86)90026-1
  contributor:
    fullname: Sims
– volume: 481
  start-page: 190
  year: 2011
  ident: 2022010809592731700_stem2308-bib-0008
  article-title: A microRNA regulon that mediates endothelial recruitment and metastasis by cancer cells
  publication-title: Nature
  doi: 10.1038/nature10661
  contributor:
    fullname: Png
– volume: 32
  start-page: D552
  year: 2004
  ident: 2022010809592731700_stem2308-bib-0022
  article-title: GenePaint.org: An atlas of gene expression patterns in the mouse embryo
  publication-title: Nucleic Acids Res
  doi: 10.1093/nar/gkh029
  contributor:
    fullname: Visel
– volume: 8
  start-page: e51243
  year: 2013
  ident: 2022010809592731700_stem2308-bib-0024
  article-title: A critical role for MAPK signalling pathways in the transcriptional regulation of toll like receptors
  publication-title: PLoS One
  doi: 10.1371/journal.pone.0051243
  contributor:
    fullname: Peroval
– volume: 27
  start-page: 4169
  year: 2013
  ident: 2022010809592731700_stem2308-bib-0027
  article-title: Extracellular HSP27 mediates angiogenesis through Toll-like receptor 3
  publication-title: FASEB J
  doi: 10.1096/fj.12-226977
  contributor:
    fullname: Thuringer
– volume: 30
  start-page: 1369
  year: 2009
  ident: 2022010809592731700_stem2308-bib-0005
  article-title: Concerted stimuli regulating osteo-chondral differentiation from stem cells: Phenotype acquisition regulated by microRNAs
  publication-title: Acta Pharmacol Sin
  doi: 10.1038/aps.2009.143
  contributor:
    fullname: Gordeladze
– volume: 29
  start-page: e45
  year: 2001
  ident: 2022010809592731700_stem2308-bib-0015
  article-title: A new mathematical model for relative quantification in real-time RT-PCR
  publication-title: Nucleic Acids Res
  doi: 10.1093/nar/29.9.e45
  contributor:
    fullname: Pfaffl
– volume: 115
  start-page: 2223
  year: 2005
  ident: 2022010809592731700_stem2308-bib-0026
  article-title: The matrix component biglycan is proinflammatory and signals through Toll-like receptors 4 and 2 in macrophages
  publication-title: J Clin Invest
  doi: 10.1172/JCI23755
  contributor:
    fullname: Schaefer
– volume: 84
  start-page: 767
  year: 2004
  ident: 2022010809592731700_stem2308-bib-0003
  article-title: Molecular regulation of vascular smooth muscle cell differentiation in development and disease
  publication-title: Physiol Rev
  doi: 10.1152/physrev.00041.2003
  contributor:
    fullname: Owens
– volume: 589
  start-page: 4681
  year: 2011
  ident: 2022010809592731700_stem2308-bib-0028
  article-title: Temporal changes in microvessel leakiness during wound healing discriminated by in vivo fluorescence recovery after photobleaching
  publication-title: J Physiol
  doi: 10.1113/jphysiol.2011.208355
  contributor:
    fullname: Machado
– volume: 46
  start-page: 72
  year: 2001
  ident: 2022010809592731700_stem2308-bib-0017
  article-title: Measurement of MAP kinase activation by flow cytometry using phospho-specific antibodies to MEK and ERK: Potential for pharmacodynamic monitoring of signal transduction inhibitors
  publication-title: Cytometry
  doi: 10.1002/cyto.1067
  contributor:
    fullname: Chow
– volume: 15
  start-page: 54
  year: 2014
  ident: 2022010809592731700_stem2308-bib-0033
  article-title: The miR-126-VEGFR2 axis controls the innate response to pathogen-associated nucleic acids
  publication-title: Nat Immunol
  doi: 10.1038/ni.2767
  contributor:
    fullname: Agudo
– volume: 132
  start-page: 2657
  year: 2005
  ident: 2022010809592731700_stem2308-bib-0010
  article-title: Perivascular cells expressing annexin A5 define a novel mesenchymal stem cell-like population with the capacity to differentiate into multiple mesenchymal lineages
  publication-title: Development
  doi: 10.1242/dev.01846
  contributor:
    fullname: Brachvogel
– start-page: 1997
  volume-title: Image J. U. S. National Institutes of Health
  ident: 2022010809592731700_stem2308-bib-0019
  contributor:
    fullname: Rasband
– volume: 162
  start-page: 156
  year: 1987
  ident: 2022010809592731700_stem2308-bib-0013
  article-title: Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction
  publication-title: Anal Biochem
  doi: 10.1016/0003-2697(87)90021-2
  contributor:
    fullname: Chomczynski
– volume: 47
  start-page: 2065
  year: 2010
  ident: 2022010809592731700_stem2308-bib-0025
  article-title: Regulation of Toll-like receptor-induced chemokine production in murine dendritic cells by mitogen-activated protein kinases
  publication-title: Mol Immunol
  doi: 10.1016/j.molimm.2010.04.004
  contributor:
    fullname: Mitchell
– volume: 302
  start-page: 1368
  year: 2003
  ident: 2022010809592731700_stem2308-bib-0031
  article-title: Targeted protein degradation and synapse remodeling by an inducible protein kinase
  publication-title: Science
  doi: 10.1126/science.1082475
  contributor:
    fullname: Pak
– volume: 8
  start-page: e53262
  year: 2010
  ident: 2022010809592731700_stem2308-bib-0001
  article-title: PECAM1(+)/Sca1(+)/CD38(+) vascular cells transform into myofibroblast-like cells in skin wound repair
  publication-title: PLoS ONE
  doi: 10.1371/journal.pone.0053262
  contributor:
    fullname: Etich
– volume: 286
  start-page: 5708
  year: 2011
  ident: 2022010809592731700_stem2308-bib-0012
  article-title: Identification of novel binding partners (annexins) for the cell death signal phosphatidylserine and definition of their recognition motif
  publication-title: J Biol Chem
  doi: 10.1074/jbc.M110.193086
  contributor:
    fullname: Rosenbaum
– volume: 26
  start-page: 1421
  year: 2012
  ident: 2022010809592731700_stem2308-bib-0029
  article-title: A shared molecular mechanism underlies the human rasopathies Legius syndrome and Neurofibromatosis-1
  publication-title: Genes Dev
  doi: 10.1101/gad.190876.112
  contributor:
    fullname: Stowe
– volume: 113
  start-page: 40
  year: 2013
  ident: 2022010809592731700_stem2308-bib-0007
  article-title: Regulation of vascular smooth muscle cell turnover by endothelial cell-secreted MicroRNA-126: Role of shear stress
  publication-title: Circ Res
  doi: 10.1161/CIRCRESAHA.113.280883
  contributor:
    fullname: Zhou
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Snippet microRNAs (miRNAs) can regulate the interplay between perivascular cells (PVC) and endothelial cells (EC) during angiogenesis, but the relevant PVC‐specific...
microRNAs (miRNAs) can regulate the interplay between perivascular cells (PVC) and endothelial cells (EC) during angiogenesis, but the relevant PVC-specific...
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SubjectTerms Angiogenesis
Animals
Blood Vessels - cytology
Cell Adhesion - drug effects
Cell Adhesion - genetics
Cell Communication - drug effects
Cell Communication - genetics
Cell Movement - drug effects
Cell Movement - genetics
Cell Shape - drug effects
Chemokines - metabolism
Coculture Techniques
Collagen - pharmacology
Drug Combinations
Extracellular Matrix - drug effects
Extracellular Matrix - metabolism
Extracellular Signal-Regulated MAP Kinases - metabolism
Gene Silencing - drug effects
Human Umbilical Vein Endothelial Cells - cytology
Human Umbilical Vein Endothelial Cells - drug effects
Human Umbilical Vein Endothelial Cells - metabolism
Humans
Laminin - pharmacology
MAP Kinase Signaling System - drug effects
Mice
MicroRNAs - genetics
MicroRNAs - metabolism
miRNA
miR‐126‐3p
Neovascularization, Physiologic - genetics
Perivascular cells
Plk2
Proteoglycans - pharmacology
Spred1
Tlr3
Transcriptome - genetics
Up-Regulation - drug effects
Up-Regulation - genetics
Title miR‐126‐3p Promotes Matrix‐Dependent Perivascular Cell Attachment, Migration and Intercellular Interaction
URI https://onlinelibrary.wiley.com/doi/abs/10.1002%2Fstem.2308
https://www.ncbi.nlm.nih.gov/pubmed/26934179
https://www.proquest.com/docview/1786773745
https://search.proquest.com/docview/1790966228
Volume 34
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