Effects of the microbial secondary metabolites pyrrolnitrin, phenazine and patulin on INS-1 rat pancreatic β-cells

• Published in: FEMS immunology and medical microbiology Vol. 63; no. 2; pp. 217 - 227
• Main Authors: Nisr, Raid B., Russell, Mark A., Chrachri, Abdesslam, Moody, A. John, Gilpin, Martyn L.
• Format: Journal Article
• Language: English
• Published: Oxford, UK Blackwell Publishing Ltd 01.11.2011; Blackwell; Oxford University Press
• Subjects: Animals; Bacteria - chemistry; Bacteria - metabolism; Bacteriology; Beta cells; Biological and medical sciences; Burkholderia; Calcium; Calcium - analysis; Calcium ions; Cell Line; Cell Survival - drug effects; Cell viability; Cystic fibrosis; Cytotoxicity; diabetes; Diabetes mellitus; Exposure; Fundamental and applied biological sciences. Psychology; Glucose; infection; Insulin; Insulin - metabolism; Insulin Secretion; insulin synthesis; Insulin-Secreting Cells - chemistry; Insulin-Secreting Cells - drug effects; Insulin-Secreting Cells - metabolism; INS‐1 cells; Membrane Potentials - drug effects; Metabolites; Microbiology; Microorganisms; Miscellaneous; Pancreas; patch clamp; Patulin; Patulin - metabolism; Patulin - toxicity; Phenazine; Phenazines - metabolism; Phenazines - toxicity; Pyrrolnitrin - metabolism; Pyrrolnitrin - toxicity; Rats; Secondary metabolites; Secretion; Time Factors; Toxicity; INS-1 cells; infection; patch clamp; insulin synthesis; diabetes; Endocrinopathy; Pancreatic hormone; Pyrrolnitrin; Rat; Metabolite; Diabetes mellitus; Mycotoxin; Rodentia; Insulin; Patulin; Toxin; Vertebrata; Antifungal agent; Mammalia; Immunology; Bacteria; Burkholderia

Abstract The effects on pancreatic β-cell viability and function of three microbial secondary metabolites pyrrolnitrin, phenazine and patulin were investigated, using the rat clonal pancreatic β-cell line, INS-1. Cells were exposed to 10-fold serial dilutions (range 0–10 µg mL−1) of the purified com...