The bifurcation of the cyanogenic glucoside and glucosinolate biosynthetic pathways

• Published in: The Plant journal : for cell and molecular biology Vol. 84; no. 3; pp. 558 - 573
• Main Authors: Clausen, Mette, Kannangara, Rubini M., Olsen, Carl E., Blomstedt, Cecilia K., Gleadow, Roslyn M., Jørgensen, Kirsten, Bak, Søren, Motawie, Mohammed S., Møller, Birger Lindberg
• Format: Journal Article
• Language: English
• Published: England Blackwell Publishing Ltd 01.11.2015
• Subjects: Arabidopsis Proteins - genetics; Arabidopsis Proteins - metabolism; Arabidopsis thaliana; biochemical pathways; Biosynthesis; Biosynthetic Pathways; Botany; cis-trans isomers; CYP71E1; CYP79A1; CYP83B1; Cytochrome P-450 Enzyme System - genetics; Cytochrome P-450 Enzyme System - metabolism; cytochrome P450; Dehydration; E‐ and Z‐oxime metabolism; glucosinolates; Glucosinolates - metabolism; Isomerism; microbial Z‐oxime‐nitrile pathway; microsomes; mutants; Mutation; Nicotiana - genetics; Nicotiana - metabolism; Nicotiana benthamiana; nitriles; oxime dehydration; Oximes - metabolism; plant pathogens; Plant Proteins - genetics; Plant Proteins - metabolism; Plant resistance; Plants, Genetically Modified; Sinapis alba; Sorghum; Sorghum - genetics; Sorghum - metabolism; Sorghum bicolor; tyrosine; Tyrosine - metabolism; CYP71E1; E- and Z-oxime metabolism; Sorghum bicolor; nitriles; CYP83B1; microbial Z-oxime-nitrile pathway; oxime dehydration; Sinapis alba; cytochrome P450; CYP79A1
• ISSN: 0960-7412; 1365-313X
• DOI: 10.1111/tpj.13023

Summary The biosynthetic pathway for the cyanogenic glucoside dhurrin in sorghum has previously been shown to involve the sequential production of (E)‐ and (Z)‐p‐hydroxyphenylacetaldoxime. In this study we used microsomes prepared from wild‐type and mutant sorghum or transiently transformed Nicotian...