A new method for histamine release from purified peripheral blood basophils using monoclonal antibody-coated magnetic beads - Nishi, Hiroshi; Nishimura, Shinji; Higashiura, Masahito; Ikeya, Norie; Ohta, Hideki; Tsuji, Tetsuo; Nishimura, Masaji; Ohnishi, Shigeo; Higashi, Hideo (Journal of immunological methods, 2000)
Keywords: Allergy testing; Antibodies, Monoclonal; Antibody Specificity; Basophil; Basophils - cytology; Basophils - immunology; Biological and medical sciences; Enzyme-Linked Immunosorbent Assay - methods; Fundamental and applied biological sciences. Psychology; Fundamental immunology; Histamine - analysis; Histamine release; Histamine Release - immunology; Humans; Hypersensitivity - diagnosis; Immediate hypersensitivity. Allergy. Anaphylaxis, etc; Immunobiology; Immunomagnetic Separation - methods; Magnetic beads; magnetism; Positive selection; Techniques; DP, Dermatophagoides pteronyssinus; Positive selection; WBC, white blood cells; Histamine release; Allergy testing; ELISA, enzyme-linked immunosorbent assay; HSA, human serum albumin; HACMG, HA with CaCl 2, MgCl 2 and glucose; Basophil; HEPES, 2-[4-(2-hydroxyethyl)-1-piperazinyl]ethanesulfonic acid; HPLC, high-performance liquid chromatography; EDTA, ethylenediaminetetraacetic acid disodium salt; HRP, horseradish peroxidase; Magnetic beads; HA, HEPES-buffered saline with HSA; Histamine; Immunomagnetic method; Laboratory investigations; Monoclonal antibody; Allergenicity; Release; Quantitative analysis; Microassay