Development of TaqMan real-time RT-PCR for detection of avian reoviruses

• Published in: Journal of virological methods Vol. 177; no. 1; pp. 75 - 79
• Main Authors: Guo, Kejun, Dormitorio, Teresa V., Ou, Shan-Chia, Giambrone, Joseph J.
• Format: Journal Article
• Language: English
• Published: Kidlington Elsevier B.V 01.10.2011; Elsevier
• Subjects: Animals; Avian reoviruses; Biological and medical sciences; birds; Detection; detection limit; Differentiation; financial economics; Fundamental and applied biological sciences. Psychology; genome; Genome, Viral - genetics; Microbiology; Orthoreovirus, Avian - genetics; Orthoreovirus, Avian - isolation & purification; pharmaceutical industry; poultry; Real-Time Polymerase Chain Reaction; Real-time RT-PCR; Reoviridae; Reproducibility of Results; Reverse Transcriptase Polymerase Chain Reaction; RNA, Viral - genetics; Sensitivity and Specificity; statistical analysis; TaqMan; Techniques used in virology; tissue culture; vaccines; Virology; viruses; Real-time RT-PCR; Avian reoviruses; TaqMan; Detection; Differentiation; Vertebrata; Method; Aves; Real time; Reverse transcription polymerase chain reaction

► Avian reoviruses (ARVs) are cause of economic losses in poultry. ► A TaqMan real-time RT-PCR assay for detection of ARVs was developed. ► This test was rapid, specific, and sensitive and will be useful in diagnostic laboratories and for all the quantitation of vaccine viruses for pharmaceutical co...