In-Depth Analysis of the Interaction of HIV-1 with Cellular microRNA Biogenesis and Effector Mechanisms

• Published in: mBio Vol. 4; no. 2; p. e000193
• Main Authors: Whisnant, Adam W., Bogerd, Hal P., Flores, Omar, Ho, Phong, Powers, Jason G., Sharova, Natalia, Stevenson, Mario, Chen, Chin-Ho, Cullen, Bryan R.
• Format: Journal Article
• Language: English
• Published: United States American Society of Microbiology 01.05.2013; American Society for Microbiology
• Subjects: binding sites; biogenesis; CD4-Positive T-Lymphocytes - virology; Cells, Cultured; crosslinking; gene expression; Gene Expression Regulation, Viral; High-Throughput Nucleotide Sequencing; HIV-1 - pathogenicity; HIV-1 - physiology; Host-Pathogen Interactions; Human immunodeficiency virus 1; Humans; macrophages; Macrophages - virology; messenger RNA; microRNA; MicroRNAs - chemistry; MicroRNAs - genetics; MicroRNAs - metabolism; precipitin tests; regulator genes; RNA, Messenger - metabolism; RNA, Viral - metabolism; T-lymphocytes

The question of how HIV-1 interfaces with cellular microRNA (miRNA) biogenesis and effector mechanisms has been highly controversial. Here, we first used deep sequencing of small RNAs present in two different infected cell lines (TZM-bl and C8166) and two types of primary human cells (CD4 + peripher...