A cell epigenotype specific model for the correction of brain cellular heterogeneity bias and its application to age, brain region and major depression

• Published in: Epigenetics Vol. 8; no. 3; pp. 290 - 302
• Main Authors: Guintivano, Jerry, Aryee, Martin J., Kaminsky, Zachary A.
• Format: Journal Article
• Language: English
• Published: United States Taylor & Francis 01.03.2013; Landes Bioscience
• Subjects: Adolescent; Adult; age; Age Factors; Brain - metabolism; Brain - pathology; brain region; Case-Control Studies; cellular heterogeneity; Depressive Disorder, Major - genetics; Depressive Disorder, Major - metabolism; DNA Methylation; Epigenesis, Genetic; epigenetics; Female; Flow Cytometry; fluorescence activated cell sorting; Genetic Markers; glia; Humans; Male; microarray; Middle Aged; Models, Genetic; neurons; Neurons - classification; Neurons - metabolism; Neurons - pathology; Research Paper; Sex Factors; brain region; microarray; DNA methylation; cellular heterogeneity; glia; neurons; age; epigenetics; fluorescence activated cell sorting

Brain cellular heterogeneity may bias cell type specific DNA methylation patterns, influencing findings in psychiatric epigenetic studies. We performed fluorescence activated cell sorting (FACS) of neuronal nuclei and Illumina HM450 DNA methylation profiling in post mortem frontal cortex of 29 major...