Hierarchical Mechanisms for Direct Reprogramming of Fibroblasts to Neurons

• Published in: Cell Vol. 155; no. 3; pp. 621 - 635
• Main Authors: Wapinski, Orly L., Vierbuchen, Thomas, Qu, Kun, Lee, Qian Yi, Chanda, Soham, Fuentes, Daniel R., Giresi, Paul G., Ng, Yi Han, Marro, Samuele, Neff, Norma F., Drechsel, Daniela, Martynoga, Ben, Castro, Diogo S., Webb, Ashley E., Südhof, Thomas C., Brunet, Anne, Guillemot, Francois, Chang, Howard Y., Wernig, Marius
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 24.10.2013
• Subjects: Animals; Basic Helix-Loop-Helix Transcription Factors - metabolism; Cell Differentiation; cells; Cellular Reprogramming; chromatin; Chromatin - metabolism; Embryo, Mammalian - cytology; fibroblasts; Fibroblasts - cytology; Fibroblasts - metabolism; Gene Regulatory Networks; genes; Genome-Wide Association Study; human diseases; Humans; medicine; Mice; Nerve Tissue Proteins - metabolism; neurons; Neurons - cytology; Neurons - metabolism; POU Domain Factors - metabolism; Repressor Proteins - metabolism; transcription factors; Transcription Factors - metabolism

Direct lineage reprogramming is a promising approach for human disease modeling and regenerative medicine, with poorly understood mechanisms. Here, we reveal a hierarchical mechanism in the direct conversion of fibroblasts into induced neuronal (iN) cells mediated by the transcription factors Ascl1,...