CRISPR‑Cas9 engineered Saccharomyces cerevisiae for endolysin delivery to combat Listeria monocytogenes

• Published in: Applied microbiology and biotechnology Vol. 109; no. 81; pp. 1 - 12
• Main Authors: Sáez Moreno, David, Cunha, Joana, Melo, Luís Daniel Rodrigues, Tanaka, Kenya, Bamba, Takahiro, Hasunuma, Tomosiha, Azeredo, Joana, Domingues, Lucília
• Format: Journal Article
• Language: English
• Published: Berlin/Heidelberg Springer Nature 02.04.2025; Springer Berlin Heidelberg; Springer Nature B.V
• Subjects: Anti-Bacterial Agents - metabolism; Anti-Bacterial Agents - pharmacology; antibacterial properties; Bacteria; bacteriophages; Bacteriophages - enzymology; Bacteriophages - genetics; Biocontrol; Biological control; Biomedical and Life Sciences; Biotechnologically Relevant Enzymes and Proteins; Biotechnology; Cell walls; Central nervous system; CRISPR; CRISPR-Cas Systems; death; Endolysin; Endopeptidases - genetics; Endopeptidases - metabolism; Endopeptidases - pharmacology; Engineered Saccharomyces cerevisiae; Food consumption; Food contamination; Gastroenteritis; gene deletion; genome; Life Sciences; Listeria; Listeria monocytogenes; Listeria monocytogenes - drug effects; Listeriosis; Listeriosis - microbiology; Listeriosis - prevention & control; Microbial Genetics and Genomics; Microbiology; milk; Phages; Plasmids; Probiotics; risk; Saccharomyces cerevisiae; Saccharomyces cerevisiae - genetics; Saccharomyces cerevisiae - metabolism; secretion; serotypes; therapeutics; Yeast; yeasts; Engineered; Endolysin; Probiotics; CRISPR-Cas9; Biocontrol; Listeria monocytogenes; Engineered Saccharomyces cerevisiae

Listeriosis is an infection caused by the consumption of food contaminated with Listeria monocytogenes. It leads to febrile gastroenteritis, central nervous system infections, and even death in risk populations. Bacteriophage endolysins selectively kill bacteria hydrolyzing their cell walls and have...