improved protocol for electroporation of Oenococcus oeni ATCC BAA-1163 using ethanol as immediate membrane fluidizing agent

• Published in: Letters in applied microbiology Vol. 47; no. 4; pp. 333 - 338
• Main Authors: Assad-García, J.S, Bonnin-Jusserand, M, Garmyn, D, Guzzo, J, Alexandre, H, Grandvalet, C
• Format: Journal Article
• Language: English
• Published: Oxford, UK Oxford, UK : Blackwell Publishing Ltd 01.10.2008; Blackwell Publishing Ltd; Blackwell Science; Oxford University Press
• Subjects: Bacterial Proteins - genetics; Biological and medical sciences; Cell Membrane - drug effects; Cell Membrane Permeability - drug effects; DNA, Bacterial - analysis; DNA, Bacterial - genetics; electroporation; Electroporation - methods; Ethanol - pharmacology; Fundamental and applied biological sciences. Psychology; Gram-Positive Asporogenous Rods - drug effects; Gram-Positive Asporogenous Rods - genetics; Life Sciences; membrane fluidizing agent; Microbiology; Microbiology and Parasitology; Oenococcus oeni; Plasmids - genetics; Lactic acid bacteria; Ethanol; Applied microbiology; Bacteria; Electroporation; Membrane; membrane fluidizing agent; Oenococcus oeni; ELECTROPORATION; MEMBRANE FLUIDIZING AGENT; OENOCOCCUS OENI

To finalize an effective and reproducible electroporation procedure to transform Oenococcus oeni ATCC BAA-1163 strain. The vector pGID052 was selected to optimize the electroporation procedure. Transformation efficiency was 5·8 x 10³ per μg of DNA. Transformation was improved when competent cells we...