Photoswitches: Key molecules for subdiffraction-resolution fluorescence imaging and molecular quantification

• Published in: Laser & photonics reviews Vol. 3; no. 1-2; pp. 180 - 202
• Main Authors: Heilemann, M., Dedecker, P., Hofkens, J., Sauer, M.
• Format: Journal Article
• Language: English
• Published: Berlin WILEY-VCH Verlag 01.02.2009; WILEY‐VCH Verlag; Wiley-VCH
• Subjects: Barriers; Biological and medical sciences; Density; Diffraction; Exact sciences and technology; Fluorescence; Fundamental and applied biological sciences. Psychology; Fundamental areas of phenomenology (including applications); General aspects; General aspects, investigation technics, apparatus; General equipment and techniques; Imaging; Instrumentation. Materials. Reagents. Research laboratory organization; Instruments, apparatus, components and techniques common to several branches of physics and astronomy; Microscopy; Molecular optical switches and photoswitches; Optical computers, logic elements, interconnects, switches; neural networks; Optical elements, devices, and systems; Optical microscopes; Optics; photoswitching microscopy; Physics; Sensors (chemical, optical, electrical, movement, gas, etc.); remote sensing; subdiffraction- or super-resolution fluorescence imaging; Tissues, organs and organisms biophysics; 87.80.Nj; Resolving power; Optical switches; Superresolution; Biological tissues; 61.05.-a; Fluorescence; Imagery; photoswitching microscopy; Proteins; 87.80.-y; Fluorophore; 87.64.M; Visible radiation; Optical sensors; Optical microscopes; 87.57.cf; Photochromic materials; Fluorescence microscopy; Biomedical imaging; Molecular optical switches and photoswitches; subdiffraction- or super-resolution fluorescence imaging.; 32.50.+d

Optical microscopes, often referred to as ‘light microscopes’, use visible light and a system of lenses to provide us with magnified images of small samples. Combined with highly sensitive fluorescence detection techniques and efficient fluorescent probes they allow the non‐invasive 3D study of subc...