Characterization of the Envelope Glycoprotein of a Novel Filovirus, Lloviu Virus

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Published inJournal of Virology Vol. 88; no. 1; pp. 99 - 109
Main Authors Maruyama, Junki, Miyamoto, Hiroko, Kajihara, Masahiro, Ogawa, Hirohito, Maeda, Ken, Sakoda, Yoshihiro, Yoshida, Reiko, Takada, Ayato
Format Journal Article
LanguageEnglish
Published United States American Society for Microbiology 01.01.2014
Subjects
Amino Acid Sequence
Animals
Base Sequence
Blotting, Western
Cross Reactions
Electrophoresis, Polyacrylamide Gel
Enzyme-Linked Immunosorbent Assay
Female
Filoviridae
Filoviridae - metabolism
Filoviridae - physiology
Filovirus
HEK293 Cells
Humans
Mice
Mice, Inbred BALB C
Microscopy, Electron, Scanning
Microscopy, Electron, Transmission
Molecular Sequence Data
Primates
Vesicular stomatitis virus
Viral Envelope Proteins - metabolism
Viral Tropism
Virus-Cell Interactions
Online AccessGet full text

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Abstract Article Usage Stats Services JVI Citing Articles Google Scholar PubMed Related Content Social Bookmarking CiteULike Delicious Digg Facebook Google+ Mendeley Reddit StumbleUpon Twitter current issue Spotlights in the Current Issue JVI About JVI Subscribers Authors Reviewers Advertisers Inquiries from the Press Permissions & Commercial Reprints ASM Journals Public Access Policy JVI RSS Feeds 1752 N Street N.W. • Washington DC 20036 202.737.3600 • 202.942.9355 fax • journals@asmusa.org Print ISSN: 0022-538X Online ISSN: 1098-5514 Copyright © 2014 by the American Society for Microbiology.   For an alternate route to JVI .asm.org, visit: JVI       
AbstractList Lloviu virus (LLOV), a novel filovirus detected in bats, is phylogenetically distinct from viruses in the genera Ebolavirus and Marburgvirus in the family Filoviridae . While filoviruses are known to cause severe hemorrhagic fever in humans and/or nonhuman primates, LLOV is biologically uncharacterized, since infectious LLOV has never been isolated. To examine the properties of LLOV, we characterized its envelope glycoprotein (GP), which likely plays a key role in viral tropism and pathogenicity. We first found that LLOV GP principally has the same primary structure as the other filovirus GPs. Similar to the other filoviruses, virus-like particles (VLPs) produced by transient expression of LLOV GP, matrix protein, and nucleoprotein in 293T cells had densely arrayed GP spikes on a filamentous particle. Mouse antiserum to LLOV VLP was barely cross-reactive to viruses of the other genera, indicating that LLOV is serologically distinct from the other known filoviruses. For functional study of LLOV GP, we utilized a vesicular stomatitis virus (VSV) pseudotype system and found that LLOV GP requires low endosomal pH and cathepsin L, and that human C-type lectins act as attachment factors for LLOV entry into cells. Interestingly, LLOV GP-pseudotyped VSV infected particular bat cell lines more efficiently than viruses bearing other filovirus GPs. These results suggest that LLOV GP mediates cellular entry in a manner similar to that of the other filoviruses while showing preferential tropism for some bat cells.
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Lloviu virus (LLOV), a novel filovirus detected in bats, is phylogenetically distinct from viruses in the genera Ebolavirus and Marburgvirus in the family Filoviridae. While filoviruses are known to cause severe hemorrhagic fever in humans and/or nonhuman primates, LLOV is biologically uncharacterized, since infectious LLOV has never been isolated. To examine the properties of LLOV, we characterized its envelope glycoprotein (GP), which likely plays a key role in viral tropism and pathogenicity. We first found that LLOV GP principally has the same primary structure as the other filovirus GPs. Similar to the other filoviruses, virus-like particles (VLPs) produced by transient expression of LLOV GP, matrix protein, and nucleoprotein in 293T cells had densely arrayed GP spikes on a filamentous particle. Mouse antiserum to LLOV VLP was barely cross-reactive to viruses of the other genera, indicating that LLOV is serologically distinct from the other known filoviruses. For functional study of LLOV GP, we utilized a vesicular stomatitis virus (VSV) pseudotype system and found that LLOV GP requires low endosomal pH and cathepsin L, and that human C-type lectins act as attachment factors for LLOV entry into cells. Interestingly, LLOV GP-pseudotyped VSV infected particular bat cell lines more efficiently than viruses bearing other filovirus GPs. These results suggest that LLOV GP mediates cellular entry in a manner similar to that of the other filoviruses while showing preferential tropism for some bat cells.Lloviu virus (LLOV), a novel filovirus detected in bats, is phylogenetically distinct from viruses in the genera Ebolavirus and Marburgvirus in the family Filoviridae. While filoviruses are known to cause severe hemorrhagic fever in humans and/or nonhuman primates, LLOV is biologically uncharacterized, since infectious LLOV has never been isolated. To examine the properties of LLOV, we characterized its envelope glycoprotein (GP), which likely plays a key role in viral tropism and pathogenicity. We first found that LLOV GP principally has the same primary structure as the other filovirus GPs. Similar to the other filoviruses, virus-like particles (VLPs) produced by transient expression of LLOV GP, matrix protein, and nucleoprotein in 293T cells had densely arrayed GP spikes on a filamentous particle. Mouse antiserum to LLOV VLP was barely cross-reactive to viruses of the other genera, indicating that LLOV is serologically distinct from the other known filoviruses. For functional study of LLOV GP, we utilized a vesicular stomatitis virus (VSV) pseudotype system and found that LLOV GP requires low endosomal pH and cathepsin L, and that human C-type lectins act as attachment factors for LLOV entry into cells. Interestingly, LLOV GP-pseudotyped VSV infected particular bat cell lines more efficiently than viruses bearing other filovirus GPs. These results suggest that LLOV GP mediates cellular entry in a manner similar to that of the other filoviruses while showing preferential tropism for some bat cells.
Lloviu virus (LLOV), a novel filovirus detected in bats, is phylogenetically distinct from viruses in the genera Ebolavirus and Marburgvirus in the family Filoviridae. While filoviruses are known to cause severe hemorrhagic fever in humans and/or nonhuman primates, LLOV is biologically uncharacterized, since infectious LLOV has never been isolated. To examine the properties of LLOV, we characterized its envelope glycoprotein (GP), which likely plays a key role in viral tropism and pathogenicity. We first found that LLOV GP principally has the same primary structure as the other filovirus GPs. Similar to the other filoviruses, virus-like particles (VLPs) produced by transient expression of LLOV GP, matrix protein, and nucleoprotein in 293T cells had densely arrayed GP spikes on a filamentous particle. Mouse antiserum to LLOV VLP was barely cross-reactive to viruses of the other genera, indicating that LLOV is serologically distinct from the other known filoviruses. For functional study of LLOV GP, we utilized a vesicular stomatitis virus (VSV) pseudotype system and found that LLOV GP requires low endosomal pH and cathepsin L, and that human C-type lectins act as attachment factors for LLOV entry into cells. Interestingly, LLOV GP-pseudotyped VSV infected particular bat cell lines more efficiently than viruses bearing other filovirus GPs. These results suggest that LLOV GP mediates cellular entry in a manner similar to that of the other filoviruses while showing preferential tropism for some bat cells.
Author Ken Maeda
Hirohito Ogawa
Masahiro Kajihara
Hiroko Miyamoto
Yoshihiro Sakoda
Reiko Yoshida
Junki Maruyama
Ayato Takada
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  fullname: Maruyama, Junki
  organization: Division of Global Epidemiology, Research Center for Zoonosis Control, Hokkaido University, Sapporo, Japan
– sequence: 2
  givenname: Hiroko
  surname: Miyamoto
  fullname: Miyamoto, Hiroko
  organization: Division of Global Epidemiology, Research Center for Zoonosis Control, Hokkaido University, Sapporo, Japan
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  givenname: Masahiro
  surname: Kajihara
  fullname: Kajihara, Masahiro
  organization: Division of Global Epidemiology, Research Center for Zoonosis Control, Hokkaido University, Sapporo, Japan
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  surname: Ogawa
  fullname: Ogawa, Hirohito
  organization: Hokudai Center for Zoonosis Control in Zambia, School of Veterinary Medicine, The University of Zambia, Lusaka, Zambia, Hokkaido University Research Center for Zoonosis Control, Sapporo, Japan
– sequence: 5
  givenname: Ken
  surname: Maeda
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  givenname: Yoshihiro
  surname: Sakoda
  fullname: Sakoda, Yoshihiro
  organization: Laboratory of Microbiology, Department of Disease Control, Graduate School of Veterinary Medicine, Hokkaido University, Sapporo, Japan
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  givenname: Reiko
  surname: Yoshida
  fullname: Yoshida, Reiko
  organization: Division of Global Epidemiology, Research Center for Zoonosis Control, Hokkaido University, Sapporo, Japan
– sequence: 8
  givenname: Ayato
  surname: Takada
  fullname: Takada, Ayato
  organization: Division of Global Epidemiology, Research Center for Zoonosis Control, Hokkaido University, Sapporo, Japan, School of Veterinary Medicine, the University of Zambia, Lusaka, Zambia
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Cites_doi 10.1128/JVI.01157-06
10.1128/JVI.76.10.4855-4865.2002
10.1038/nature10380
10.1016/0168-1702(95)00080-1
10.1074/jbc.M706292200
10.1016/0378-1119(91)90434-D
10.1126/science.1172705
10.1128/JVI.77.2.1337-1346.2003
10.1073/pnas.1019030108
10.1128/JVI.06346-11
10.1073/pnas.94.26.14764
10.1073/pnas.93.8.3602
10.1128/CVI.00170-10
10.3201/eid1312.071115
10.1006/viro.2002.1730
10.1093/infdis/jir300
10.1074/jbc.M202104200
10.3201/eid1402.070932
10.1128/JVI.76.13.6841-6844.2002
10.1006/viro.1995.0052
10.1371/journal.ppat.0020099
10.1007/s11626-998-0053-6
10.1371/journal.pntd.0001923
10.1186/1743-422X-3-31
10.1371/journal.ppat.1002304
10.1371/journal.pone.0061904
10.1128/JVI.75.5.2324-2330.2001
10.1371/journal.ppat.1002877
10.1002/rmv.405
10.1186/1471-2334-9-159
10.4049/jimmunol.156.1.128
10.1086/520581
10.1371/journal.ppat.1000536
10.1126/science.1110656
10.1128/JVI.02021-09
10.1093/infdis/jir334
10.1016/j.virusres.2013.05.004
10.1086/520607
10.1073/pnas.95.10.5762
10.1016/j.virol.2006.06.041
10.1006/viro.1994.1147
10.1128/JVI.76.18.9135-9142.2002
10.1017/S1462399404008300
10.1182/blood-2006-09-048058
10.1093/glycob/2.4.299
10.1016/0168-1702(92)90027-7
10.1128/JVI.78.6.2943-2947.2004
10.1007/s00705-012-1477-6
10.1006/viro.1999.0110
10.1128/JVI.72.4.3155-3160.1998
10.1016/0042-6822(85)90236-3
10.1016/S0140-6736(10)60667-8
10.1038/438575a
10.1038/nature10348
10.1126/science.1066371
10.1371/journal.pone.0000764
10.1007/978-3-642-59949-1_8
10.1016/j.bbrc.2010.10.136
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References e_1_3_2_26_2
e_1_3_2_49_2
e_1_3_2_28_2
e_1_3_2_41_2
e_1_3_2_20_2
e_1_3_2_43_2
e_1_3_2_22_2
e_1_3_2_45_2
e_1_3_2_24_2
e_1_3_2_47_2
e_1_3_2_9_2
e_1_3_2_16_2
e_1_3_2_37_2
e_1_3_2_7_2
e_1_3_2_18_2
e_1_3_2_39_2
e_1_3_2_54_2
e_1_3_2_10_2
e_1_3_2_31_2
e_1_3_2_52_2
e_1_3_2_5_2
e_1_3_2_12_2
e_1_3_2_33_2
e_1_3_2_58_2
e_1_3_2_3_2
e_1_3_2_14_2
e_1_3_2_35_2
e_1_3_2_56_2
e_1_3_2_27_2
e_1_3_2_48_2
e_1_3_2_29_2
e_1_3_2_40_2
e_1_3_2_21_2
e_1_3_2_42_2
e_1_3_2_23_2
e_1_3_2_44_2
Suzuki N (e_1_3_2_50_2) 1996; 156
e_1_3_2_25_2
e_1_3_2_46_2
e_1_3_2_15_2
e_1_3_2_38_2
e_1_3_2_8_2
e_1_3_2_17_2
e_1_3_2_59_2
e_1_3_2_6_2
e_1_3_2_19_2
e_1_3_2_30_2
e_1_3_2_53_2
e_1_3_2_32_2
e_1_3_2_51_2
e_1_3_2_11_2
e_1_3_2_34_2
e_1_3_2_57_2
e_1_3_2_4_2
e_1_3_2_13_2
e_1_3_2_36_2
e_1_3_2_55_2
e_1_3_2_2_2
21987779 - J Infect Dis. 2011 Nov;204 Suppl 3:S978-85
16719918 - Virol J. 2006;3:31
11160735 - J Virol. 2001 Mar;75(5):2324-30
17712412 - PLoS One. 2007;2(8):e764
20861331 - Clin Vaccine Immunol. 2010 Nov;17(11):1723-8
21987755 - J Infect Dis. 2011 Nov;204 Suppl 3:S804-9
18258034 - Emerg Infect Dis. 2007 Dec;13(12):1847-51
12050398 - J Virol. 2002 Jul;76(13):6841-4
17940970 - J Infect Dis. 2007 Nov 15;196 Suppl 2:S347-56
9405687 - Proc Natl Acad Sci U S A. 1997 Dec 23;94(26):14764-9
16989883 - Virology. 2007 Feb 5;358(1):79-88
21536871 - Proc Natl Acad Sci U S A. 2011 May 17;108(20):8426-31
4060597 - Virology. 1985 Nov;147(1):169-76
21866101 - Nature. 2011 Sep 15;477(7364):344-8
9525641 - J Virol. 1998 Apr;72(4):3155-60
15831716 - Science. 2005 Jun 10;308(5728):1643-5
17005688 - J Virol. 2006 Oct;80(20):10109-16
12186897 - J Virol. 2002 Sep;76(18):9135-42
21866103 - Nature. 2011 Sep 15;477(7364):340-3
17940955 - J Infect Dis. 2007 Nov 15;196 Suppl 2:S237-46
8122375 - Virology. 1994 Mar;199(2):469-73
18258142 - Emerg Infect Dis. 2008 Feb;14(2):347-9
19785757 - BMC Infect Dis. 2009;9:159
20219911 - J Virol. 2010 May;84(10):5140-7
11739956 - Science. 2001 Dec 7;294(5549):2163-6
8598452 - J Immunol. 1996 Jan 1;156(1):128-35
22039362 - PLoS Pathog. 2011 Oct;7(10):e1002304
11919201 - J Biol Chem. 2002 Jun 7;277(23):20686-93
23055920 - PLoS Pathog. 2012;8(10):e1002877
14625886 - Rev Med Virol. 2003 Nov-Dec;13(6):387-98
12504546 - Virology. 2003 Jan 5;305(1):115-23
17009868 - PLoS Pathog. 2006 Sep;2(9):e99
16319873 - Nature. 2005 Dec 1;438(7068):575-6
21084112 - Lancet. 2011 Mar 5;377(9768):849-62
23626748 - PLoS One. 2013;8(4):e61904
15383160 - Expert Rev Mol Med. 2004 Sep 21;6(20):1-24
22238307 - J Virol. 2012 Mar;86(6):3284-92
12502850 - J Virol. 2003 Jan;77(2):1337-46
8837880 - Virus Res. 1995 Dec;39(2-3):129-50
23236527 - PLoS Negl Trop Dis. 2012;6(12):e1923
17339424 - Blood. 2007 Jun 15;109(12):5337-45
9576958 - Proc Natl Acad Sci U S A. 1998 May 12;95(10):5762-7
17984090 - J Biol Chem. 2008 Jan 4;283(1):593-602
9542636 - In Vitro Cell Dev Biol Anim. 1998 Jan;34(1):53-7
19590002 - Science. 2009 Jul 10;325(5937):204-6
8553543 - Virology. 1995 Dec 20;214(2):421-30
10683320 - Virology. 2000 Mar 1;268(1):1-6
8622982 - Proc Natl Acad Sci U S A. 1996 Apr 16;93(8):3602-7
21056544 - Biochem Biophys Res Commun. 2010 Dec 3;403(1):144-8
1660837 - Gene. 1991 Dec 15;108(2):193-9
1421752 - Glycobiology. 1992 Aug;2(4):299-312
23702199 - Virus Res. 2013 Sep;176(1-2):83-90
9893382 - Curr Top Microbiol Immunol. 1999;235:117-43
22996641 - Arch Virol. 2014 May;159(5):1129-32
19649327 - PLoS Pathog. 2009 Jul;5(7):e1000536
11967302 - J Virol. 2002 May;76(10):4855-65
14990712 - J Virol. 2004 Mar;78(6):2943-7
1626422 - Virus Res. 1992 Jun;24(1):1-19
References_xml – ident: e_1_3_2_56_2
  doi: 10.1128/JVI.01157-06
– ident: e_1_3_2_27_2
  doi: 10.1128/JVI.76.10.4855-4865.2002
– ident: e_1_3_2_57_2
  doi: 10.1038/nature10380
– ident: e_1_3_2_30_2
  doi: 10.1016/0168-1702(95)00080-1
– ident: e_1_3_2_45_2
  doi: 10.1074/jbc.M706292200
– ident: e_1_3_2_24_2
  doi: 10.1016/0378-1119(91)90434-D
– ident: e_1_3_2_51_2
  doi: 10.1126/science.1172705
– ident: e_1_3_2_14_2
  doi: 10.1128/JVI.77.2.1337-1346.2003
– ident: e_1_3_2_55_2
  doi: 10.1073/pnas.1019030108
– ident: e_1_3_2_41_2
  doi: 10.1128/JVI.06346-11
– ident: e_1_3_2_7_2
  doi: 10.1073/pnas.94.26.14764
– ident: e_1_3_2_22_2
  doi: 10.1073/pnas.93.8.3602
– ident: e_1_3_2_26_2
  doi: 10.1128/CVI.00170-10
– ident: e_1_3_2_59_2
  doi: 10.3201/eid1312.071115
– ident: e_1_3_2_43_2
  doi: 10.1006/viro.2002.1730
– ident: e_1_3_2_52_2
  doi: 10.1093/infdis/jir300
– ident: e_1_3_2_49_2
  doi: 10.1074/jbc.M202104200
– ident: e_1_3_2_19_2
  doi: 10.3201/eid1402.070932
– ident: e_1_3_2_12_2
  doi: 10.1128/JVI.76.13.6841-6844.2002
– ident: e_1_3_2_23_2
  doi: 10.1006/viro.1995.0052
– ident: e_1_3_2_28_2
  doi: 10.1371/journal.ppat.0020099
– ident: e_1_3_2_18_2
  doi: 10.1007/s11626-998-0053-6
– ident: e_1_3_2_42_2
  doi: 10.1371/journal.pntd.0001923
– ident: e_1_3_2_29_2
  doi: 10.1186/1743-422X-3-31
– ident: e_1_3_2_5_2
  doi: 10.1371/journal.ppat.1002304
– ident: e_1_3_2_54_2
  doi: 10.1371/journal.pone.0061904
– ident: e_1_3_2_32_2
  doi: 10.1128/JVI.75.5.2324-2330.2001
– ident: e_1_3_2_35_2
  doi: 10.1371/journal.ppat.1002877
– ident: e_1_3_2_33_2
  doi: 10.1002/rmv.405
– ident: e_1_3_2_38_2
  doi: 10.1186/1471-2334-9-159
– volume: 156
  start-page: 128
  year: 1996
  ident: e_1_3_2_50_2
  article-title: Molecular cloning and expression of cDNA encoding human macrophage C-type lectin. Its unique carbohydrate binding specificity for Tn antigen
  publication-title: J. Immunol.
  doi: 10.4049/jimmunol.156.1.128
– ident: e_1_3_2_15_2
  doi: 10.1086/520581
– ident: e_1_3_2_34_2
  doi: 10.1371/journal.ppat.1000536
– ident: e_1_3_2_31_2
  doi: 10.1126/science.1110656
– ident: e_1_3_2_21_2
  doi: 10.1128/JVI.02021-09
– ident: e_1_3_2_16_2
  doi: 10.1093/infdis/jir334
– ident: e_1_3_2_25_2
  doi: 10.1016/j.virusres.2013.05.004
– ident: e_1_3_2_47_2
  doi: 10.1086/520607
– ident: e_1_3_2_39_2
  doi: 10.1073/pnas.95.10.5762
– ident: e_1_3_2_9_2
  doi: 10.1016/j.virol.2006.06.041
– ident: e_1_3_2_11_2
  doi: 10.1006/viro.1994.1147
– ident: e_1_3_2_13_2
  doi: 10.1128/JVI.76.18.9135-9142.2002
– ident: e_1_3_2_4_2
  doi: 10.1017/S1462399404008300
– ident: e_1_3_2_44_2
  doi: 10.1182/blood-2006-09-048058
– ident: e_1_3_2_10_2
  doi: 10.1093/glycob/2.4.299
– ident: e_1_3_2_17_2
  doi: 10.1016/0168-1702(92)90027-7
– ident: e_1_3_2_20_2
  doi: 10.1128/JVI.78.6.2943-2947.2004
– ident: e_1_3_2_53_2
  doi: 10.1007/s00705-012-1477-6
– ident: e_1_3_2_40_2
  doi: 10.1006/viro.1999.0110
– ident: e_1_3_2_8_2
  doi: 10.1128/JVI.72.4.3155-3160.1998
– ident: e_1_3_2_6_2
  doi: 10.1016/0042-6822(85)90236-3
– ident: e_1_3_2_2_2
  doi: 10.1016/S0140-6736(10)60667-8
– ident: e_1_3_2_37_2
  doi: 10.1038/438575a
– ident: e_1_3_2_58_2
  doi: 10.1038/nature10348
– ident: e_1_3_2_48_2
  doi: 10.1126/science.1066371
– ident: e_1_3_2_36_2
  doi: 10.1371/journal.pone.0000764
– ident: e_1_3_2_3_2
  doi: 10.1007/978-3-642-59949-1_8
– ident: e_1_3_2_46_2
  doi: 10.1016/j.bbrc.2010.10.136
– reference: 1626422 - Virus Res. 1992 Jun;24(1):1-19
– reference: 8622982 - Proc Natl Acad Sci U S A. 1996 Apr 16;93(8):3602-7
– reference: 12502850 - J Virol. 2003 Jan;77(2):1337-46
– reference: 11967302 - J Virol. 2002 May;76(10):4855-65
– reference: 19590002 - Science. 2009 Jul 10;325(5937):204-6
– reference: 21866101 - Nature. 2011 Sep 15;477(7364):344-8
– reference: 16319873 - Nature. 2005 Dec 1;438(7068):575-6
– reference: 1421752 - Glycobiology. 1992 Aug;2(4):299-312
– reference: 10683320 - Virology. 2000 Mar 1;268(1):1-6
– reference: 15831716 - Science. 2005 Jun 10;308(5728):1643-5
– reference: 14625886 - Rev Med Virol. 2003 Nov-Dec;13(6):387-98
– reference: 22996641 - Arch Virol. 2014 May;159(5):1129-32
– reference: 14990712 - J Virol. 2004 Mar;78(6):2943-7
– reference: 15383160 - Expert Rev Mol Med. 2004 Sep 21;6(20):1-24
– reference: 17712412 - PLoS One. 2007;2(8):e764
– reference: 21084112 - Lancet. 2011 Mar 5;377(9768):849-62
– reference: 9542636 - In Vitro Cell Dev Biol Anim. 1998 Jan;34(1):53-7
– reference: 8598452 - J Immunol. 1996 Jan 1;156(1):128-35
– reference: 11739956 - Science. 2001 Dec 7;294(5549):2163-6
– reference: 23236527 - PLoS Negl Trop Dis. 2012;6(12):e1923
– reference: 9576958 - Proc Natl Acad Sci U S A. 1998 May 12;95(10):5762-7
– reference: 17339424 - Blood. 2007 Jun 15;109(12):5337-45
– reference: 1660837 - Gene. 1991 Dec 15;108(2):193-9
– reference: 17940955 - J Infect Dis. 2007 Nov 15;196 Suppl 2:S237-46
– reference: 21987779 - J Infect Dis. 2011 Nov;204 Suppl 3:S978-85
– reference: 21056544 - Biochem Biophys Res Commun. 2010 Dec 3;403(1):144-8
– reference: 21987755 - J Infect Dis. 2011 Nov;204 Suppl 3:S804-9
– reference: 21536871 - Proc Natl Acad Sci U S A. 2011 May 17;108(20):8426-31
– reference: 19785757 - BMC Infect Dis. 2009;9:159
– reference: 20219911 - J Virol. 2010 May;84(10):5140-7
– reference: 16989883 - Virology. 2007 Feb 5;358(1):79-88
– reference: 8122375 - Virology. 1994 Mar;199(2):469-73
– reference: 17005688 - J Virol. 2006 Oct;80(20):10109-16
– reference: 17984090 - J Biol Chem. 2008 Jan 4;283(1):593-602
– reference: 9525641 - J Virol. 1998 Apr;72(4):3155-60
– reference: 8837880 - Virus Res. 1995 Dec;39(2-3):129-50
– reference: 9405687 - Proc Natl Acad Sci U S A. 1997 Dec 23;94(26):14764-9
– reference: 23626748 - PLoS One. 2013;8(4):e61904
– reference: 12050398 - J Virol. 2002 Jul;76(13):6841-4
– reference: 22039362 - PLoS Pathog. 2011 Oct;7(10):e1002304
– reference: 4060597 - Virology. 1985 Nov;147(1):169-76
– reference: 16719918 - Virol J. 2006;3:31
– reference: 11919201 - J Biol Chem. 2002 Jun 7;277(23):20686-93
– reference: 23055920 - PLoS Pathog. 2012;8(10):e1002877
– reference: 12504546 - Virology. 2003 Jan 5;305(1):115-23
– reference: 17009868 - PLoS Pathog. 2006 Sep;2(9):e99
– reference: 22238307 - J Virol. 2012 Mar;86(6):3284-92
– reference: 8553543 - Virology. 1995 Dec 20;214(2):421-30
– reference: 21866103 - Nature. 2011 Sep 15;477(7364):340-3
– reference: 11160735 - J Virol. 2001 Mar;75(5):2324-30
– reference: 19649327 - PLoS Pathog. 2009 Jul;5(7):e1000536
– reference: 9893382 - Curr Top Microbiol Immunol. 1999;235:117-43
– reference: 20861331 - Clin Vaccine Immunol. 2010 Nov;17(11):1723-8
– reference: 17940970 - J Infect Dis. 2007 Nov 15;196 Suppl 2:S347-56
– reference: 18258034 - Emerg Infect Dis. 2007 Dec;13(12):1847-51
– reference: 18258142 - Emerg Infect Dis. 2008 Feb;14(2):347-9
– reference: 23702199 - Virus Res. 2013 Sep;176(1-2):83-90
– reference: 12186897 - J Virol. 2002 Sep;76(18):9135-42
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Snippet Article Usage Stats Services JVI Citing Articles Google Scholar PubMed Related Content Social Bookmarking CiteULike Delicious Digg Facebook Google+ Mendeley...
Lloviu virus (LLOV), a novel filovirus detected in bats, is phylogenetically distinct from viruses in the genera Ebolavirus and Marburgvirus in the family...
Lloviu virus (LLOV), a novel filovirus detected in bats, is phylogenetically distinct from viruses in the genera Ebolavirus and Marburgvirus in the family...
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StartPage 99
SubjectTerms Amino Acid Sequence
Animals
Base Sequence
Blotting, Western
Cross Reactions
Electrophoresis, Polyacrylamide Gel
Enzyme-Linked Immunosorbent Assay
Female
Filoviridae
Filoviridae - metabolism
Filoviridae - physiology
Filovirus
HEK293 Cells
Humans
Mice
Mice, Inbred BALB C
Microscopy, Electron, Scanning
Microscopy, Electron, Transmission
Molecular Sequence Data
Primates
Vesicular stomatitis virus
Viral Envelope Proteins - metabolism
Viral Tropism
Virus-Cell Interactions
Title Characterization of the Envelope Glycoprotein of a Novel Filovirus, Lloviu Virus
URI http://jvi.asm.org/content/88/1/99.abstract
https://www.ncbi.nlm.nih.gov/pubmed/24131711
https://www.proquest.com/docview/1490739198
https://www.proquest.com/docview/1492645513
https://pubmed.ncbi.nlm.nih.gov/PMC3911722
Volume 88
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