Immunolocalization of caspase proteolysis in situ: evidence for widespread caspase-mediated apoptosis of neurons and glia in the postnatal rat brain

• Published in: Neuroscience Vol. 92; no. 4; pp. 1425 - 1442
• Main Authors: Siman, R, Bozyczko-Coyne, D, Meyer, S.L, Bhat, R.V
• Format: Journal Article
• Language: English
• Published: Oxford Elsevier Ltd 01.01.1999; Elsevier
• Subjects: Animals; Animals, Newborn - physiology; Antibodies, Monoclonal; apoptosis; Apoptosis - physiology; Biological and medical sciences; Brain - cytology; Brain - enzymology; caspase; Caspases - metabolism; Caspases - physiology; Cell Nucleus - immunology; Cell Nucleus - ultrastructure; Cells, Cultured; Development. Senescence. Regeneration. Transplantation; Fluorescent Antibody Technique, Direct; Fundamental and applied biological sciences. Psychology; growth factor deprivation; Immunoblotting; Immunohistochemistry; Nerve Tissue Proteins - metabolism; Neuroglia - physiology; Neurons - physiology; PC12 cell; PC12 Cells; postnatal development; programmed cell death; Proto-Oncogene Proteins c-jun - biosynthesis; Rats; Rats, Sprague-Dawley; Vertebrates: nervous system and sense organs; growth factor deprivation; EGL, external granule cell layer; IGL, internal granule cell layer; HEPES, N-2-hydroxyethylpiperazine- N′-2-ethanesulfonic acid; PC12 cell; PARP, poly(ADP-ribose)polymerase; apoptosis; NGF, nerve growth factor; TBS, Tris-buffered saline; caspase; postnatal development; IgG, immunoglobulin G; PB, phosphate buffer; PBS, phosphate-buffered saline; PND, postnatal day; programmed cell death; EGTA, ethyleneglycolbis(aminoethyl ether)tetra-acetate; TUNEL, terminal transferase-mediated dUTP–biotin nick end labeling; Glial cell; Deprivation; Rat; Enzyme; Neuroglia; Rodentia; Central nervous system; Postnatal development; Vertebrata; Mammalia; Neuron; Proteolysis; Apoptosis; Brain (vertebrata); Growth factor

Activation of a member of the caspase family of cysteine proteases is thought to be required for the execution of apoptosis in neurons and other cell types. We describe here an antibody (Ab127) reactive with a neoantigenic site on caspase substrate proteins degraded during apoptosis, and its charact...