Functional analysis of the disulphide loop mutant of staphylococcal enterotoxin C2

• Published in: Applied microbiology and biotechnology Vol. 82; no. 5; pp. 861 - 871
• Main Authors: Wang, Xiaogang, Xu, Mingkai, Cai, Yongming, Yang, Hongli, Zhang, Huiwen, Zhang, Chenggang
• Format: Journal Article
• Language: English
• Published: Berlin/Heidelberg Berlin/Heidelberg : Springer-Verlag 01.04.2009; Springer Berlin Heidelberg; Springer; Springer Nature B.V
• Subjects: Amino acids; Animals; Antigens; Biocompatibility; Biological activity; Biological and medical sciences; Biotechnologically Relevant Enzymes and Proteins; Biotechnology; Cats; Cell growth; Cell Line; Cell Proliferation; chemistry; Cytotoxicity; disulfide bonds; Disulfides; Disulfides - immunology; E coli; enterotoxins; Enterotoxins - chemistry; Enterotoxins - genetics; Enterotoxins - physiology; Enterotoxins - toxicity; Enzyme-Linked Immunosorbent Assay; Enzymes; Epitopes; Escherichia coli; Female; Functional analysis; Fundamental and applied biological sciences. Psychology; genetics; immunology; Immunotherapy; Laboratory animals; Life Sciences; Lymphocytes; Lymphocytes T; Major histocompatibility complex; metabolism; Mice; Mice, Inbred BALB C; Microbial Genetics and Genomics; Microbiology; Mutagenesis; Mutagenesis, Site-Directed; Mutant Proteins; Mutant Proteins - chemistry; Mutant Proteins - physiology; Mutant Proteins - toxicity; Mutants; neoplasm cells; neoplasms; Pallets; Pharmaceuticals; physiology; Plasmids; Protein expression; Protein Structure, Tertiary; Protein Structure, Tertiary - physiology; Proteins; Rabbits; Roles; Site-directed mutagenesis; Staphylococcal enterotoxin C2; Staphylococcus aureus; Staphylococcus aureus - genetics; Staphylococcus aureus - immunology; Staphylococcus aureus - metabolism; Studies; Superantigen; Superantigens; Superantigens - chemistry; Superantigens - physiology; Superantigens - toxicity; T cell receptors; T-lymphocytes; T-Lymphocytes - physiology; The disulphide loop; Toxicity; Tumors; Superantigen; The disulphide loop; Toxicity; Immunotherapy; Site-directed mutagenesis; Staphylococcal enterotoxin C2; Site directed mutagenesis; Functional analysis; Staphylococcus; Toxin; Enterotoxin; Bacteria; Micrococcales; Micrococcaceae; Mutation

The superantigen staphylococcal enterotoxin C2 (SEC2) tremendously activate T lymphocytes bearing certain T-cell receptor Vβ domains when binding to MHC II molecules, which launches a powerful response of tumour inhibition in vitro as well as in vivo. However, the toxicity of SEC2 performed in clini...