Protein disulfide isomerase and glutathione are alternative substrates in the one Cys catalytic cycle of glutathione peroxidase 7

• Published in: Biochimica et biophysica acta Vol. 1830; no. 6; pp. 3846 - 3857
• Main Authors: Bosello-Travain, Valentina, Conrad, Marcus, Cozza, Giorgio, Negro, Alessandro, Quartesan, Silvia, Rossetto, Monica, Roveri, Antonella, Toppo, Stefano, Ursini, Fulvio, Zaccarin, Mattia, Maiorino, Matilde
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 01.06.2013
• Subjects: Animals; Carrier Proteins - chemistry; Carrier Proteins - genetics; Carrier Proteins - metabolism; Catalysis; endoplasmic reticulum; enzyme substrates; glutathione; Glutathione - chemistry; Glutathione - genetics; Glutathione - metabolism; Glutathione peroxidase; Humans; Kinetics; Mice; Molecular Docking Simulation; molecular models; Mutation; oxidation; Oxidation-Reduction; Peroxidases - chemistry; Peroxidases - genetics; Peroxidases - metabolism; Peroxide; phospholipids; protein disulfide-isomerase; Protein Disulfide-Isomerases - chemistry; Protein Disulfide-Isomerases - genetics; Protein Disulfide-Isomerases - metabolism; protein-protein interactions; proteins; Recombinant Fusion Proteins - chemistry; Recombinant Fusion Proteins - genetics; Recombinant Fusion Proteins - metabolism; Redox switch; Redoxin; substrate specificity; Substrate Specificity - genetics; surface plasmon resonance; GPxs; SecGPxs; GPx7; PCOOH; PDI; Peroxide; Trx; UP; GSH; TrxR; Glutathione peroxidase; GSSG; Prdxs; 2CysGPxs; 1CysGPx; Syn; CP; ER; CR; CysGPxs; ROOH; Sec; Redoxin; Redox switch; Kinetics

Mammalian GPx7 is a monomeric glutathione peroxidase of the endoplasmic reticulum (ER), containing a Cys redox center (CysGPx). Although containing a peroxidatic Cys (CP) it lacks the resolving Cys (CR), that confers fast reactivity with thioredoxin (Trx) or related proteins to most other CysGPxs. R...