Glycated albumin triggers fibrosis and apoptosis via an NADPH oxidase/Nox4-MAPK pathway-dependent mechanism in renal proximal tubular cells

• Published in: Molecular and cellular endocrinology Vol. 405; pp. 74 - 83
• Main Authors: Qi, Weiwei, Niu, Jianying, Qin, Qiaojing, Qiao, Zhongdong, Gu, Yong
• Format: Journal Article
• Language: English
• Published: Ireland Elsevier Ireland Ltd 15.04.2015
• Subjects: Acetophenones - pharmacology; acetylcysteine; albumins; Animals; Apoptosis; caspase-3; Cell Line; Cellular; Damage; diabetic nephropathy; disease course; fibronectins; Fibronectins - genetics; Fibronectins - metabolism; Fibrosis; Gene Expression; glycation; hyperglycemia; in vitro studies; Inhibitors; Kidney Tubules, Proximal - enzymology; Kidney Tubules, Proximal - pathology; kidneys; MAP Kinase Signaling System; MAPK pathway; messenger RNA; mitogen-activated protein kinase; NAD(P)H oxidase (H2O2-forming); NADPH oxidase; NADPH Oxidase 4; NADPH Oxidases - antagonists & inhibitors; NADPH Oxidases - genetics; NADPH Oxidases - metabolism; Nox4; Oxidase; Pathways; patients; phosphorylation; quantitative polymerase chain reaction; Rats; reactive oxygen species; Serum Albumin - physiology; signal transduction; small interfering RNA; transforming growth factor beta; Transforming Growth Factor beta - genetics; Transforming Growth Factor beta - metabolism; Tubulointerstitial fibrosis; MAPK pathway; Apo; FN; DN; OSS; ECM; AS-IV; Tubulointerstitial fibrosis; NADPH oxidase; ROS; Nox4; GA; Apoptosis

•Glycated albumin induced fibrosis and apoptosis in NRK-52E cells via oxidative stress.•NADPH oxidase inhibition or Nox4 gene silence ameliorated glycated albumin induced cellular fibrosis andapoptosis.•MAPK pathways involved in NADPH oxidase/Nox 4 induced NRK-52E cells fibrosis and apoptosis stimul...