A serum-free culture medium production system by co-culture combining growth factor-secreting cells and l-lactate-assimilating cyanobacteria for sustainable cultured meat production

• Published in: Scientific reports Vol. 14; no. 1; pp. 19578 - 10
• Main Authors: Chu, Shanga, Haraguchi, Yuji, Asahi, Toru, Kato, Yuichi, Kondo, Akihiko, Hasunuma, Tomohisa, Shimizu, Tatsuya
• Format: Journal Article
• Language: English
• Published: London Nature Publishing Group UK 23.08.2024; Nature Publishing Group; Nature Portfolio
• Subjects: 631/1647/2234; 631/61; 631/61/168; 631/80; Amino acids; Ammonium; Animals; Apoptosis; Cell culture; Cell Line; Cell Proliferation; Co-culture; Coculture Techniques - methods; Culture media; Culture Media, Serum-Free; Cultured meat; Growth factors; Hepatocytes; Humanities and Social Sciences; In Vitro Meat; Intercellular Signaling Peptides and Proteins - genetics; Intercellular Signaling Peptides and Proteins - metabolism; l-lactate-assimilating cyanobacterium; Lactate permease; Lactic acid; Lactic Acid - metabolism; Mammalian cell cultures; Meat; Meat production; Metabolites; Monoculture; multidisciplinary; Myoblasts; Myoblasts - cytology; Myoblasts - metabolism; Nutrients; Permease; Pyruvic acid; Rats; RL34 cells; Science; Science (multidisciplinary); Serum replacement; Synechococcus - genetics; Synechococcus - growth & development; Synechococcus - metabolism; RL34 cells; lactate-assimilating cyanobacterium; Co-culture; Serum replacement; Cultured meat; Mammalian cell cultures; l-lactate-assimilating cyanobacterium

Large-scale production of cultured meat requires bulk culture medium containing growth-promoting proteins from animal serum. However, animal serum for mammalian cell culture is associated with high costs, ethical concerns, and contamination risks. Owing to its growth factor content, conditioned medi...