Correction of multiple-blinking artifacts in photoactivated localization microscopy

• Published in: Nature methods Vol. 19; no. 5; pp. 594 - 602
• Main Authors: Jensen, Louis G., Hoh, Tjun Yee, Williamson, David J., Griffié, Juliette, Sage, Daniel, Rubin-Delanchy, Patrick, Owen, Dylan M.
• Format: Journal Article
• Language: English
• Published: New York Nature Publishing Group US 01.05.2022; Nature Publishing Group
• Subjects: 631/1647/245/2225; 631/1647/328/2238; Adapter proteins; Algorithms; Bioinformatics; Biological Microscopy; Biological Techniques; Biomedical and Life Sciences; Biomedical Engineering/Biotechnology; Blinking; Cell activation; Chemical compounds; Clustering; Fluorescence; Fluorophores; Image analysis; Image processing; Immunological synapses; Immunology; Life Sciences; Localization; Lymphocytes; Lymphocytes T; Microscopy; Proteins; Proteomics; Synapses; Workflow
• ISSN: 1548-7091; 1548-7105; 1548-7105
• DOI: 10.1038/s41592-022-01463-w

Photoactivated localization microscopy (PALM) produces an array of localization coordinates by means of photoactivatable fluorescent proteins. However, observations are subject to fluorophore multiple blinking and each protein is included in the dataset an unknown number of times at different positi...