Morphokinetic analysis of pronuclei using time-lapse cinematography in bovine zygotes

• Published in: Theriogenology Vol. 166; pp. 55 - 63
• Main Authors: Suzuki, Ryosuke, Okada, Mai, Nagai, Hiroki, Kobayashi, Junichi, Sugimura, Satoshi
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 01.05.2021
• Subjects: animal reproduction; blastocyst; Cattle; Delipidation; Embryo; embryogenesis; light; light microscopy; lipids; Morphokinetics; Pronuclei; pronucleus; Pronuclei; Morphokinetics; Embryo; Delipidation; Cattle

The morphokinetics of pronuclei (PN) are considered crucial factors affecting embryogenesis in mammals. Whereas, since bovine zygotes contain a large number of cytosolic lipid droplets, detailed observation of PN has not been performed. In this study, we visualized PN using time-lapse cinematography...