Label-free assessment of complement-dependent cytotoxicity of therapeutic antibodies via a whole-cell MALDI mass spectrometry bioassay

• Published in: Scientific reports Vol. 14; no. 1; pp. 21462 - 11
• Main Authors: Schmidt, Stefan, Geisel, Alexander, Enzlein, Thomas, Fröhlich, Björn C., Pritchett, Louise, Verneret, Melanie, Graf, Christian, Hopf, Carsten
• Format: Journal Article
• Language: English
• Published: London Nature Publishing Group UK 13.09.2024; Nature Publishing Group; Nature Portfolio
• Subjects: 631/154/1435; 631/154/436; 631/154/51; 631/154/53; 631/61/51; Adenosine Triphosphate - metabolism; Antibodies, Monoclonal; ATP; Bioassays; Biological Assay - methods; Biopharmaceuticals; Complement System Proteins - metabolism; Complement-dependent cytotoxicity; Cytotoxicity; Drug screening; Drug-response curves; Glutathione; Glutathione - metabolism; Humanities and Social Sciences; Humans; Immunotherapy; Ionization; Label-free cell assay; MALDI; Mass spectrometry; Mass spectroscopy; Metabolites; Monoclonal antibodies; Monoclonal antibody; multidisciplinary; R&D; Reagents; Research & development; Rituximab; Rituximab - pharmacology; Science; Science (multidisciplinary); Scientific imaging; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization - methods; Monoclonal antibody; Label-free cell assay; Mass spectrometry; MALDI; Complement-dependent cytotoxicity; Drug-response curves

Potency assessment of monoclonal antibodies or corresponding biosimilars in cell-based assays is an essential prerequisite in biopharmaceutical research and development. However, cellular bioassays are still subject to limitations in sample throughput, speed, and often need costly reagents or labels...