Immune Reactivity of Human Sera to the Glycoprotein B of Human Herpesvirus 7

Article Usage Stats Services JCM Citing Articles Google Scholar PubMed Related Content Social Bookmarking CiteULike Delicious Digg Facebook Google+ Mendeley Reddit StumbleUpon Twitter current issue JCM About JCM Subscribers Authors Reviewers Advertisers Inquiries from the Press Permissions & Com...

Full description

Saved in:
Bibliographic Details
Published inJournal of Clinical Microbiology Vol. 40; no. 1; pp. 44 - 51
Main Authors Franti, Michael, Aubin, Jean-Thierry, De Saint-Maur, Guillemette, Kosuge, Haruhiko, Yamanishi, Koichi, Gautheret-Dejean, Agnes, Garbarg-Chenon, Antoine, Huraux, Jean-Marie, Agut, Henri
Format Journal Article
LanguageEnglish
Published Washington, DC American Society for Microbiology 01.01.2002
Subjects
Adult
Antibodies, Viral - blood
Antibodies, Viral - immunology
Antigens, Viral - immunology
Biological and medical sciences
Carcinogenesis, carcinogens and anticarcinogens
Child, Preschool
Enzyme-Linked Immunosorbent Assay
Fundamental and applied biological sciences. Psychology
glycoprotein B
Herpesvirus 7, Human - immunology
Human herpesvirus 7
Human viral diseases
Humans
Immune Sera - immunology
Immunoblotting
Infant
Infectious diseases
Medical sciences
Microbiology
Peptides - chemical synthesis
Peptides - immunology
Recombinant Proteins - genetics
Recombinant Proteins - immunology
Roseolovirus Infections - diagnosis
Roseolovirus Infections - immunology
Tumors
Viral diseases
Viral diseases of the lymphoid tissue and the blood. Aids
Viral Envelope Proteins - chemistry
Viral Envelope Proteins - genetics
Viral Envelope Proteins - immunology
Virology
Viruses
Human
Glycoprotein
Online AccessGet full text

Cover

Abstract Article Usage Stats Services JCM Citing Articles Google Scholar PubMed Related Content Social Bookmarking CiteULike Delicious Digg Facebook Google+ Mendeley Reddit StumbleUpon Twitter current issue JCM About JCM Subscribers Authors Reviewers Advertisers Inquiries from the Press Permissions & Commercial Reprints ASM Journals Public Access Policy JCM RSS Feeds 1752 N Street N.W. • Washington DC 20036 202.737.3600 • 202.942.9355 fax • journals@asmusa.org Print ISSN: 0095-1137 Online ISSN: 1098-660X Copyright © 2014 by the American Society for Microbiology.   For an alternate route to JCM .asm.org, visit: JCM       
AbstractList The glycoprotein B (gB) is highly conserved among distinct human herpesvirus 7 (HHV-7) strains. Similarly to other herpesvirus glycoproteins, gB has been assumed to induce a specific human immune response. However, it did not appear as an immunodominant protein in conventional immunoblot assays. Recombinant gB, obtained from either Escherichia coli or baculovirus expression systems, did react specifically with HHV-7-seropositive sera, and the main corresponding epitopes were located in its N-terminal part. A 24-amino-acid peptide, corresponding to a predicted hydrophilicity peak and presenting no extensive homology with other betaherpesvirus glycoproteins, was selected in this region at positions 129 to 152 of the gB sequence. When tested by enzyme-linked immunosorbent assay (ELISA), this peptide specifically reacted with HHV-7-seropositive sera. This reactivity was significantly inhibited by the preincubation of sera with the peptide itself, lysates of gB-expressing cells, or lysates of HHV-7-infected cells. The reactivity was not significantly modified when sera were preincubated with lysates of either human cytomegalovirus (HCMV)- or HHV-6-infected cells. In cross-sectional studies including both children and adults, 49 out of 61 serum samples (80%) were found to be positive by HHV-7 ELISA, independent of their reactivity to HCMV. A longitudinal serological study of 17 children during the first 4 years of life showed that the level of ELISA-detected antibodies significantly decreased within a few weeks after birth and then increased in the following months, likely reflecting, respectively, the loss of maternal antibodies and the occurrence of seroconversion. These results demonstrate that gB peptide ELISA might be a useful tool for the serological study of HHV-7 infection.
Article Usage Stats Services JCM Citing Articles Google Scholar PubMed Related Content Social Bookmarking CiteULike Delicious Digg Facebook Google+ Mendeley Reddit StumbleUpon Twitter current issue JCM About JCM Subscribers Authors Reviewers Advertisers Inquiries from the Press Permissions & Commercial Reprints ASM Journals Public Access Policy JCM RSS Feeds 1752 N Street N.W. • Washington DC 20036 202.737.3600 • 202.942.9355 fax • journals@asmusa.org Print ISSN: 0095-1137 Online ISSN: 1098-660X Copyright © 2014 by the American Society for Microbiology.   For an alternate route to JCM .asm.org, visit: JCM       
ABSTRACT The glycoprotein B (gB) is highly conserved among distinct human herpesvirus 7 (HHV-7) strains. Similarly to other herpesvirus glycoproteins, gB has been assumed to induce a specific human immune response. However, it did not appear as an immunodominant protein in conventional immunoblot assays. Recombinant gB, obtained from either Escherichia coli or baculovirus expression systems, did react specifically with HHV-7-seropositive sera, and the main corresponding epitopes were located in its N-terminal part. A 24-amino-acid peptide, corresponding to a predicted hydrophilicity peak and presenting no extensive homology with other betaherpesvirus glycoproteins, was selected in this region at positions 129 to 152 of the gB sequence. When tested by enzyme-linked immunosorbent assay (ELISA), this peptide specifically reacted with HHV-7-seropositive sera. This reactivity was significantly inhibited by the preincubation of sera with the peptide itself, lysates of gB-expressing cells, or lysates of HHV-7-infected cells. The reactivity was not significantly modified when sera were preincubated with lysates of either human cytomegalovirus (HCMV)- or HHV-6-infected cells. In cross-sectional studies including both children and adults, 49 out of 61 serum samples (80%) were found to be positive by HHV-7 ELISA, independent of their reactivity to HCMV. A longitudinal serological study of 17 children during the first 4 years of life showed that the level of ELISA-detected antibodies significantly decreased within a few weeks after birth and then increased in the following months, likely reflecting, respectively, the loss of maternal antibodies and the occurrence of seroconversion. These results demonstrate that gB peptide ELISA might be a useful tool for the serological study of HHV-7 infection.
The glycoprotein B (gB) is highly conserved among distinct human herpesvirus 7 (HHV-7) strains. Similarly to other herpesvirus glycoproteins, gB has been assumed to induce a specific human immune response. However, it did not appear as an immunodominant protein in conventional immunoblot assays. Recombinant gB, obtained from either Escherichia coli or baculovirus expression systems, did react specifically with HHV-7-seropositive sera, and the main corresponding epitopes were located in its N-terminal part. A 24-amino-acid peptide, corresponding to a predicted hydrophilicity peak and presenting no extensive homology with other betaherpesvirus glycoproteins, was selected in this region at positions 129 to 152 of the gB sequence. When tested by enzyme-linked immunosorbent assay (ELISA), this peptide specifically reacted with HHV-7- seropositive sera. This reactivity was significantly inhibited by the preincubation of sera with the peptide itself, lysates of gB-expressing cells, or lysates of HHV-7-infected cells. The reactivity was not significantly modified when sera were preincubated with lysates of either human cytomegalovirus (HCMV)- or HHV-6-infected cells. In cross-sectional studies including both children and adults, 49 out of 61 serum samples (80%) were found to be positive by HHV-7 ELISA, independent of their reactivity to HCMV. A longitudinal serological study of 17 children during the first 4 years of life showed that the level of ELISA-detected antibodies significantly decreased within a few weeks after birth and then increased in the following months, likely reflecting, respectively, the loss of maternal antibodies and the occurrence of seroconversion. These results demonstrate that gB peptide ELISA might be a useful tool for the serological study of HHV-7 infection.
Author Haruhiko Kosuge
Henri Agut
Jean-Marie Huraux
Michael Franti
Jean-Thierry Aubin
Guillemette De Saint-Maur
Agnes Gautheret-Dejean
Antoine Garbarg-Chenon
Koichi Yamanishi
AuthorAffiliation Laboratoire de Virologie, C.E.R.V.I., UPRES EA 2387, Hôpital Pitié-Salpétrière, 75651 Paris Cedex 13, 1 Laboratoire de Microbiologie, Unité de Virologie, Hôpital Armand Trousseau, 75571 Paris Cedex 12, France, 2 Department of Microbiology, Osaka University Medical School, Suita, Osaka, Japan 3
AuthorAffiliation_xml – name: Laboratoire de Virologie, C.E.R.V.I., UPRES EA 2387, Hôpital Pitié-Salpétrière, 75651 Paris Cedex 13, 1 Laboratoire de Microbiologie, Unité de Virologie, Hôpital Armand Trousseau, 75571 Paris Cedex 12, France, 2 Department of Microbiology, Osaka University Medical School, Suita, Osaka, Japan 3
Author_xml – sequence: 1
  givenname: Michael
  surname: Franti
  fullname: Franti, Michael
  organization: Laboratoire de Virologie, C.E.R.V.I., UPRES EA 2387, Hôpital Pitié-Salpétrière, 75651 Paris Cedex 13
– sequence: 2
  givenname: Jean-Thierry
  surname: Aubin
  fullname: Aubin, Jean-Thierry
  organization: Laboratoire de Virologie, C.E.R.V.I., UPRES EA 2387, Hôpital Pitié-Salpétrière, 75651 Paris Cedex 13
– sequence: 3
  givenname: Guillemette
  surname: De Saint-Maur
  fullname: De Saint-Maur, Guillemette
  organization: Laboratoire de Microbiologie, Unité de Virologie, Hôpital Armand Trousseau, 75571 Paris Cedex 12, France
– sequence: 4
  givenname: Haruhiko
  surname: Kosuge
  fullname: Kosuge, Haruhiko
  organization: Laboratoire de Virologie, C.E.R.V.I., UPRES EA 2387, Hôpital Pitié-Salpétrière, 75651 Paris Cedex 13
– sequence: 5
  givenname: Koichi
  surname: Yamanishi
  fullname: Yamanishi, Koichi
  organization: Department of Microbiology, Osaka University Medical School, Suita, Osaka, Japan
– sequence: 6
  givenname: Agnes
  surname: Gautheret-Dejean
  fullname: Gautheret-Dejean, Agnes
  organization: Laboratoire de Virologie, C.E.R.V.I., UPRES EA 2387, Hôpital Pitié-Salpétrière, 75651 Paris Cedex 13
– sequence: 7
  givenname: Antoine
  surname: Garbarg-Chenon
  fullname: Garbarg-Chenon, Antoine
  organization: Laboratoire de Microbiologie, Unité de Virologie, Hôpital Armand Trousseau, 75571 Paris Cedex 12, France
– sequence: 8
  givenname: Jean-Marie
  surname: Huraux
  fullname: Huraux, Jean-Marie
  organization: Laboratoire de Virologie, C.E.R.V.I., UPRES EA 2387, Hôpital Pitié-Salpétrière, 75651 Paris Cedex 13
– sequence: 9
  givenname: Henri
  surname: Agut
  fullname: Agut, Henri
  organization: Laboratoire de Virologie, C.E.R.V.I., UPRES EA 2387, Hôpital Pitié-Salpétrière, 75651 Paris Cedex 13
BackLink http://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=13457602$$DView record in Pascal Francis
https://www.ncbi.nlm.nih.gov/pubmed/11773091$$D View this record in MEDLINE/PubMed
BookMark eNqFkU1vEzEQhi1URNPCL0ACX-C2weOP9e6BA0TQFAUhQQ_cLK8zblztrlN7Nyj_ni0JBE6cZqR53vl6L8hZH3sk5DmwOQCv3nxafJ7LKZ9LWSiYc8b4IzIDVldFWbLvZ2TGWK0KAKHPyUXOd4yBlEo9IecAWgtWw4ysrrtu7JF-ReuGsAvDnkZPl2Nne_oNk6VDpMMG6VW7d3Gb4oChp-9PzBLTFvMupDFT_ZQ89rbN-OwYL8nNxw83i2Wx-nJ1vXi3Kpys1FBI4V1ZNpyrCjw0HL2VNchyrWoQay9l09RcNLXV2Fhf2ppz4NpOSyM4XopL8vbQdjs2Ha4d9kOyrdmm0Nm0N9EG82-lDxtzG3cGph9VctK_PupTvB8xD6YL2WHb2h7jmI0GoUAL-C8IFReV4tUEigPoUsw5of-zDDDzYJaZzDJyyo2URoF5MGtSvfj7jpPm6M4EvDoCNjvb-mR7F_KJE1Lp8lejlwduE243P0JCY3Nn7lz3e6T4CXH-qV0
CODEN JCMIDW
CitedBy_id crossref_primary_10_1128_AEM_00511_10
crossref_primary_10_1128_JVI_76_20_10553_10558_2002
crossref_primary_10_1111_ajt_12008
Cites_doi 10.1073/pnas.91.9.3872
10.1212/WNL.52.5.1077
10.1073/pnas.87.2.748
10.1093/infdis/164.5.835
10.1128/JCM.37.12.3980-3985.1999
10.1128/JVI.72.11.8725-8730.1998
10.1002/jmv.1890400416
10.1016/S0168-1702(97)00102-0
10.1002/jmv.1890370403
10.1128/jvi.71.8.5758-5763.1997
10.1099/0022-1317-77-3-511
10.1128/jvi.66.9.5290-5297.1992
10.1006/viro.1996.0408
10.1128/JVI.74.10.4530-4540.2000
10.1006/viro.1994.1291
10.1099/0022-1317-75-10-2719
10.1542/peds.95.2.187
10.1128/cdli.3.1.79-83.1996
10.1016/0042-6822(92)90258-Q
10.1128/jvi.64.3.1079-1085.1990
10.1093/infdis/168.1.251
10.1099/0022-1317-78-4-847
10.1016/S0022-3476(94)70113-X
10.1128/jvi.65.11.6260-6265.1991
10.1128/CDLI.6.2.216-223.1999
10.1128/jvi.63.1.403-410.1989
10.1006/viro.1994.1371
10.1128/jvi.66.5.3206-3209.1992
10.1006/viro.1998.9105
10.1128/jcm.34.9.2320-2321.1996
10.1128/jvi.71.6.4571-4580.1997
10.1097/00043426-199903000-00018
10.1128/JVI.73.11.9655-9658.1999
10.1073/pnas.89.21.10552
10.1046/j.1365-2141.1998.00718.x
10.1002/jmv.1890450307
10.1002/(SICI)1096-9071(199601)48:1<88::AID-JMV14>3.0.CO;2-2
10.1099/0022-1317-74-3-495
10.1097/00006454-199411000-00016
10.1002/jmv.1890410412
10.1016/S0168-1702(96)01395-0
10.1128/jvi.70.9.5975-5989.1996
10.1111/j.1651-2227.1997.tb08942.x
ContentType Journal Article
Copyright 2002 INIST-CNRS
Copyright © 2002, American Society for Microbiology 2002
Copyright_xml – notice: 2002 INIST-CNRS
– notice: Copyright © 2002, American Society for Microbiology 2002
DBID IQODW
CGR
CUY
CVF
ECM
EIF
NPM
AAYXX
CITATION
7U9
H94
7X8
5PM
DOI 10.1128/JCM.40.1.44-51.2002
DatabaseName Pascal-Francis
Medline
MEDLINE
MEDLINE (Ovid)
MEDLINE
MEDLINE
PubMed
CrossRef
Virology and AIDS Abstracts
AIDS and Cancer Research Abstracts
MEDLINE - Academic
PubMed Central (Full Participant titles)
DatabaseTitle MEDLINE
Medline Complete
MEDLINE with Full Text
PubMed
MEDLINE (Ovid)
CrossRef
AIDS and Cancer Research Abstracts
Virology and AIDS Abstracts
MEDLINE - Academic
DatabaseTitleList

CrossRef
AIDS and Cancer Research Abstracts
MEDLINE
Database_xml – sequence: 1
  dbid: NPM
  name: PubMed
  url: https://proxy.k.utb.cz/login?url=http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed
  sourceTypes: Index Database
– sequence: 2
  dbid: EIF
  name: MEDLINE
  url: https://proxy.k.utb.cz/login?url=https://www.webofscience.com/wos/medline/basic-search
  sourceTypes: Index Database
DeliveryMethod fulltext_linktorsrc
Discipline Medicine
Biology
EISSN 1098-660X
EndPage 51
ExternalDocumentID 10_1128_JCM_40_1_44_51_2002
11773091
13457602
jcm_40_1_44
Genre Evaluation Studies
Research Support, Non-U.S. Gov't
Journal Article
GroupedDBID ---
.55
.GJ
08R
0R~
18M
29K
2WC
39C
3O-
4.4
41~
53G
5GY
5RE
5VS
AAUGY
AAYOK
ABOCM
ABPPZ
ACGFO
ADBBV
AENEX
AFMIJ
AGCDD
AI.
ALMA_UNASSIGNED_HOLDINGS
AOIJS
BAWUL
BTFSW
CS3
D-I
DIK
DU5
E3Z
EBS
EJD
F5P
FRP
GX1
H13
HF~
HYE
HZ~
H~9
IQODW
KQ8
L7B
O9-
OHT
OK1
P2P
P6G
RHF
RHI
RNS
RPM
RSF
TR2
UCJ
VH1
W8F
WHG
WOQ
X7M
ZA5
ZCA
ZGI
ZXP
~KM
AGVNZ
CGR
CUY
CVF
ECM
EIF
NPM
AAYXX
CITATION
7U9
H94
7X8
5PM
ID FETCH-LOGICAL-c485t-43fc66b22581f1b2efa49146d5913df44bb923b9a7ebaf6a922127a117e1c263
IEDL.DBID RPM
ISSN 0095-1137
IngestDate Tue Sep 17 21:21:33 EDT 2024
Sat Aug 17 00:13:26 EDT 2024
Fri Aug 16 08:41:40 EDT 2024
Thu Sep 12 17:45:48 EDT 2024
Sat Sep 28 08:34:10 EDT 2024
Sun Oct 22 16:06:39 EDT 2023
Wed May 18 15:27:06 EDT 2016
IsDoiOpenAccess false
IsOpenAccess true
IsPeerReviewed true
IsScholarly true
Issue 1
Keywords Human
Glycoprotein
Language English
License CC BY 4.0
LinkModel DirectLink
MergedId FETCHMERGED-LOGICAL-c485t-43fc66b22581f1b2efa49146d5913df44bb923b9a7ebaf6a922127a117e1c263
Notes ObjectType-Article-2
SourceType-Scholarly Journals-1
ObjectType-Feature-1
content type line 23
ObjectType-Undefined-1
ObjectType-Feature-3
Corresponding author. Mailing address: Laboratoire de Virologie, C.E.R.V.I., Hôpital Pitié-Salpétrière, 83 bd de l’Hôpital, 75651 Paris Cedex 13, France. Phone: 33-1-42 17 74 01. Fax: 33-1-42 17 74 11. E-mail: henri.agut@psl.ap-hop-paris.fr.
Present address: LFB, Les Ulis, 91958 Courtaboeuf Cedex, France.
OpenAccessLink https://doi.org/10.1128/jcm.40.1.44-51.2002
PMID 11773091
PQID 18238528
PQPubID 23462
PageCount 8
ParticipantIDs proquest_miscellaneous_18238528
highwire_asm_jcm_40_1_44
proquest_miscellaneous_71351731
pubmed_primary_11773091
pubmedcentral_primary_oai_pubmedcentral_nih_gov_120084
pascalfrancis_primary_13457602
crossref_primary_10_1128_JCM_40_1_44_51_2002
PublicationCentury 2000
PublicationDate 20020101
2002
2002-Jan
2002-01-00
PublicationDateYYYYMMDD 2002-01-01
PublicationDate_xml – month: 01
  year: 2002
  text: 20020101
  day: 01
PublicationDecade 2000
PublicationPlace Washington, DC
PublicationPlace_xml – name: Washington, DC
– name: United States
PublicationTitle Journal of Clinical Microbiology
PublicationTitleAlternate J Clin Microbiol
PublicationYear 2002
Publisher American Society for Microbiology
Publisher_xml – name: American Society for Microbiology
References e_1_3_2_26_2
e_1_3_2_49_2
e_1_3_2_27_2
e_1_3_2_48_2
e_1_3_2_28_2
e_1_3_2_29_2
e_1_3_2_41_2
e_1_3_2_40_2
e_1_3_2_43_2
e_1_3_2_21_2
e_1_3_2_42_2
e_1_3_2_22_2
e_1_3_2_45_2
e_1_3_2_23_2
e_1_3_2_44_2
e_1_3_2_24_2
e_1_3_2_47_2
e_1_3_2_25_2
e_1_3_2_46_2
e_1_3_2_15_2
e_1_3_2_38_2
e_1_3_2_16_2
e_1_3_2_37_2
e_1_3_2_7_2
e_1_3_2_17_2
e_1_3_2_6_2
e_1_3_2_18_2
e_1_3_2_39_2
e_1_3_2_19_2
(e_1_3_2_9_2) 1997; 20
e_1_3_2_32_2
e_1_3_2_10_2
e_1_3_2_31_2
e_1_3_2_5_2
e_1_3_2_11_2
e_1_3_2_34_2
e_1_3_2_4_2
e_1_3_2_33_2
e_1_3_2_3_2
e_1_3_2_13_2
e_1_3_2_36_2
e_1_3_2_2_2
e_1_3_2_14_2
e_1_3_2_35_2
(e_1_3_2_20_2) 1992; 76
(e_1_3_2_30_2) 1992; 72
(e_1_3_2_12_2) 1998; 197
(e_1_3_2_8_2) 1996; 19
References_xml – ident: e_1_3_2_26_2
  doi: 10.1073/pnas.91.9.3872
– volume: 197
  start-page: 275
  year: 1998
  ident: e_1_3_2_12_2
  publication-title: Dermatology
– ident: e_1_3_2_44_2
  doi: 10.1212/WNL.52.5.1077
– ident: e_1_3_2_19_2
  doi: 10.1073/pnas.87.2.748
– ident: e_1_3_2_31_2
  doi: 10.1093/infdis/164.5.835
– ident: e_1_3_2_39_2
  doi: 10.1128/JCM.37.12.3980-3985.1999
– ident: e_1_3_2_17_2
  doi: 10.1128/JVI.72.11.8725-8730.1998
– volume: 76
  start-page: 259
  year: 1992
  ident: e_1_3_2_20_2
  publication-title: Dev. Biol. Stand.
– ident: e_1_3_2_22_2
  doi: 10.1002/jmv.1890400416
– ident: e_1_3_2_5_2
  doi: 10.1016/S0168-1702(97)00102-0
– ident: e_1_3_2_10_2
  doi: 10.1002/jmv.1890370403
– ident: e_1_3_2_38_2
  doi: 10.1128/jvi.71.8.5758-5763.1997
– volume: 20
  start-page: 187
  year: 1997
  ident: e_1_3_2_9_2
  publication-title: New Microbiol.
– ident: e_1_3_2_16_2
  doi: 10.1099/0022-1317-77-3-511
– ident: e_1_3_2_45_2
  doi: 10.1128/jvi.66.9.5290-5297.1992
– ident: e_1_3_2_40_2
  doi: 10.1006/viro.1996.0408
– ident: e_1_3_2_37_2
  doi: 10.1128/JVI.74.10.4530-4540.2000
– ident: e_1_3_2_43_2
  doi: 10.1006/viro.1994.1291
– ident: e_1_3_2_15_2
  doi: 10.1099/0022-1317-75-10-2719
– ident: e_1_3_2_2_2
  doi: 10.1542/peds.95.2.187
– ident: e_1_3_2_4_2
  doi: 10.1128/cdli.3.1.79-83.1996
– volume: 72
  start-page: 1985
  year: 1992
  ident: e_1_3_2_30_2
  publication-title: J. Gen. Virol.
– ident: e_1_3_2_14_2
  doi: 10.1016/0042-6822(92)90258-Q
– ident: e_1_3_2_7_2
  doi: 10.1128/jvi.64.3.1079-1085.1990
– ident: e_1_3_2_11_2
  doi: 10.1093/infdis/168.1.251
– ident: e_1_3_2_25_2
  doi: 10.1099/0022-1317-78-4-847
– ident: e_1_3_2_41_2
  doi: 10.1016/S0022-3476(94)70113-X
– ident: e_1_3_2_48_2
  doi: 10.1128/jvi.65.11.6260-6265.1991
– ident: e_1_3_2_46_2
  doi: 10.1128/CDLI.6.2.216-223.1999
– ident: e_1_3_2_6_2
  doi: 10.1128/jvi.63.1.403-410.1989
– ident: e_1_3_2_35_2
  doi: 10.1006/viro.1994.1371
– ident: e_1_3_2_47_2
  doi: 10.1128/jvi.66.5.3206-3209.1992
– ident: e_1_3_2_27_2
  doi: 10.1006/viro.1998.9105
– ident: e_1_3_2_32_2
  doi: 10.1128/jcm.34.9.2320-2321.1996
– ident: e_1_3_2_36_2
  doi: 10.1128/jvi.71.6.4571-4580.1997
– ident: e_1_3_2_33_2
  doi: 10.1097/00043426-199903000-00018
– ident: e_1_3_2_18_2
  doi: 10.1128/JVI.73.11.9655-9658.1999
– ident: e_1_3_2_3_2
  doi: 10.1073/pnas.89.21.10552
– ident: e_1_3_2_34_2
  doi: 10.1046/j.1365-2141.1998.00718.x
– ident: e_1_3_2_29_2
  doi: 10.1002/jmv.1890450307
– ident: e_1_3_2_42_2
  doi: 10.1002/(SICI)1096-9071(199601)48:1<88::AID-JMV14>3.0.CO;2-2
– volume: 19
  start-page: 1
  year: 1996
  ident: e_1_3_2_8_2
  publication-title: New Microbiol.
– ident: e_1_3_2_13_2
  doi: 10.1099/0022-1317-74-3-495
– ident: e_1_3_2_23_2
  doi: 10.1097/00006454-199411000-00016
– ident: e_1_3_2_49_2
  doi: 10.1002/jmv.1890410412
– ident: e_1_3_2_21_2
  doi: 10.1016/S0168-1702(96)01395-0
– ident: e_1_3_2_28_2
  doi: 10.1128/jvi.70.9.5975-5989.1996
– ident: e_1_3_2_24_2
  doi: 10.1111/j.1651-2227.1997.tb08942.x
SSID ssj0014455
Score 1.7305934
Snippet Article Usage Stats Services JCM Citing Articles Google Scholar PubMed Related Content Social Bookmarking CiteULike Delicious Digg Facebook Google+ Mendeley...
The glycoprotein B (gB) is highly conserved among distinct human herpesvirus 7 (HHV-7) strains. Similarly to other herpesvirus glycoproteins, gB has been...
ABSTRACT The glycoprotein B (gB) is highly conserved among distinct human herpesvirus 7 (HHV-7) strains. Similarly to other herpesvirus glycoproteins, gB has...
SourceID pubmedcentral
proquest
crossref
pubmed
pascalfrancis
highwire
SourceType Open Access Repository
Aggregation Database
Index Database
Publisher
StartPage 44
SubjectTerms Adult
Antibodies, Viral - blood
Antibodies, Viral - immunology
Antigens, Viral - immunology
Biological and medical sciences
Carcinogenesis, carcinogens and anticarcinogens
Child, Preschool
Enzyme-Linked Immunosorbent Assay
Fundamental and applied biological sciences. Psychology
glycoprotein B
Herpesvirus 7, Human - immunology
Human herpesvirus 7
Human viral diseases
Humans
Immune Sera - immunology
Immunoblotting
Infant
Infectious diseases
Medical sciences
Microbiology
Peptides - chemical synthesis
Peptides - immunology
Recombinant Proteins - genetics
Recombinant Proteins - immunology
Roseolovirus Infections - diagnosis
Roseolovirus Infections - immunology
Tumors
Viral diseases
Viral diseases of the lymphoid tissue and the blood. Aids
Viral Envelope Proteins - chemistry
Viral Envelope Proteins - genetics
Viral Envelope Proteins - immunology
Virology
Viruses
Title Immune Reactivity of Human Sera to the Glycoprotein B of Human Herpesvirus 7
URI http://jcm.asm.org/content/40/1/44.abstract
https://www.ncbi.nlm.nih.gov/pubmed/11773091
https://search.proquest.com/docview/18238528
https://search.proquest.com/docview/71351731
https://pubmed.ncbi.nlm.nih.gov/PMC120084
Volume 40
hasFullText 1
inHoldings 1
isFullTextHit
isPrint
link http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwnV3fb9MwELbYJBAviI0fC7DhBx5JW8dnO36EaqNMFCE0pL1ZtmOLojWpmhZp_z22k1A6wQtvlnK2FN_ZvvOdvw-hN5VglWWxTkdYn4ORRa6JK3NjvQULk9J3Vb6f-ewbXF6z6_5RWNuXVdbWLEb1zXJUL76n2srV0o6HOrHxl_mUxKQ9jA_QgaB0iND7zAEA61gLJMsJoaJHGgrb8PhyOh9BaI8AcpZiw8RlQ0Swckn2D6YBLDjWSuo2TJfveC7-5ojeraf844C6eIwe9Z4lftf9wRG65-pjdL_jmrw9Rg_mfRb9Cfr0MT4Kcfiri68aInkEbjxO1_k47B0abxoc_EL84ebWNgnIYVHj9zuZmVuvXPtzsd62WDxFVxfnV9NZ3rMq5BZKtsmBesu5Ceu4JJ6YwnkNMuyXFZOEVh7ABIVRI7VwRnuuZRFB4HWYJkdswekzdFg3tTtBWBo34dpEgHcJvPAl01xXwnk6MSGqNBl6O8yoWnXYGSrFHEWpgi4UhLYCUIxEMswiQyfDrCvdLtUPuxxEMnS2p4XdcBRCwBT7vh7UosL6iEkPXbtm26oQP9GSFeW_JRJJoaAkQ887Ne5G700jQ3xPwb8FIjb3_pdgsgmjuzPRF__b8SV6mGhn0l3PK3S4WW_dafB-NuYsmfsv52EByA
link.rule.ids 230,315,733,786,790,891,4043,27956,27957,27958,53827,53829
linkProvider National Library of Medicine
linkToHtml http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwnV1Lj9MwEB7BIh4XHstjy2PXB44krZNxHkeoWLpLu0KoK-3Nsh1bFLZJ1aRIy6_HdhJKV3CAWyQ_JHvG45nM-PsAXhcpKxRzdTqpMgHKPAoE1VkglVGocJSZtsr3LJmc4-kFu-gehdVdWWWp5CIsL5dhufjiaytXSzXs68SGn2Zj6pL2OLwJt-xxjdI-Ru9yB4is5S3IWUBpnHZYQ9YQD0_HsxDtd4gYMB8dejYbmlo9z-nu1dTDBbtqSVHbDTMt08WfXNHrFZW_XVHHD-C8X1xbmfIt3DQyVD-u4T7-6-ofwv3OZyVv29ZHcEOX-3C7ZbG82oc7sy4__ximJ-65iSaftXsv4WgpSGWITxQQa5UEaSpiPU7y4fJKVR4iYlGSd9s-E71e6fr7Yr2pSfoE5sfv5-NJ0PE1BAoz1gQYG5Uk0lqIjBoqI20E5tYSFyyncWEQpVWFWOYi1VKYROSRg5cXdvs1VVESP4W9sir1AZBc6lEipIOOzzGJTMZEIopUm3gkbbwqB_CmlxRftagc3EczUcatjDnab47IGXU0m9EADnppclEv-Ve17LsM4HBHutvpYrShmBt71Iub25Pn0imi1NWm5jYyizMWZX_v4ekP05gO4FmrHtvZO5UbQLKjOL86ONTv3RarDh79uxX_8_8deAR3J_PZlE9Pzj6-gHue3Mb_UXoJe816o19ZH6uRh_5I_QRpSyN-
linkToPdf http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwnV3fb9MwED6NISZe-DEYy4DNDzyStE7sJH6EQunGOk1oSOPJsh1bFNakatJJ46_HdhJKJ3jZm6WcLSV3Pt_lzt8H8KbIaKGo69PJlAmJZHEosM5DqYwiigxz03b5nqWTr-Tkkl5uQd7fhfFN-0rOovJqHpWz7763cjFXg75PbHA-HWFXtCeDRWEG9-C-3bIx6_P0rn5ACG25CxgNMU6yDm_IOuPByWgaETuOCAmpzxA9ow3OrK0zvHk89ZDBrmNS1PajmZbt4l_h6O2uyr-OqfFj-Na_YNud8jNaNTJSv25hP97lCzyBR13sit61Ek9hS5e78KBls7zZhZ1pV6d_BqfH7tqJRl-0uzfh6ClQZZAvGCDrnQRqKmQjT_Tp6kZVHipiVqL3a5mJXi50fT1brmqUPYeL8ceL0STseBtCRXLahCQxKk2l9RQ5NljG2gjCrEcuKMNJYQiR1iQSyUSmpTCpYLGDmRdWBRqrOE32YLusSr0PiEk9TIV0EPKMpLHJqUhFkWmTDKXNW2UAb3tt8UWLzsF9VhPn3OqZEzvmhHCKHd1mHMB-r1Eu6jn_oea9SACHGxpeL5cQm5K5uUe9yrndga6sIkpdrWpuM7Qkp3H-fwlPg5glOIAXrYmsV-_MLoB0w3j-CDj0780n1iQ8CnhrAgd3nXgEO-cfxvz0-OzzS3joOW78j6VXsN0sV_q1DbUaeeh31W-XKyX-
openUrl ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fsummon.serialssolutions.com&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Ajournal&rft.genre=article&rft.atitle=Immune+reactivity+of+human+sera+to+the+glycoprotein+B+of+human+herpesvirus+7&rft.jtitle=Journal+of+clinical+microbiology&rft.au=FRANTI%2C+Michael&rft.au=AUBIN%2C+Jean-Thierry&rft.au=DE+SAINT-MAUR%2C+Guillemette&rft.au=KOSUGE%2C+Haruhiko&rft.date=2002&rft.pub=American+Society+for+Microbiology&rft.issn=0095-1137&rft.eissn=1098-660X&rft.volume=40&rft.issue=1&rft.spage=44&rft.epage=51&rft_id=info:doi/10.1128%2FJCM.40.1.44-51.2002&rft.externalDBID=n%2Fa&rft.externalDocID=13457602
thumbnail_l http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/lc.gif&issn=0095-1137&client=summon
thumbnail_m http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/mc.gif&issn=0095-1137&client=summon
thumbnail_s http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/sc.gif&issn=0095-1137&client=summon