Anti-HIV-1 activity and structure–activity-relationship study of a fucosylated glycosaminoglycan from an echinoderm by targeting the conserved CD4 induced epitope

• Published in: Biochimica et biophysica acta Vol. 1830; no. 10; pp. 4681 - 4691
• Main Authors: Lian, Wu, Wu, Mingyi, Huang, Ning, Gao, Na, Xiao, Chuang, Li, Zi, Zhang, Zhigang, Zheng, Yongtang, Peng, Wenlie, Zhao, Jinhua
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 01.10.2013
• Subjects: Animals; Anti-HIV Agents - chemistry; Anti-HIV Agents - pharmacology; Anti-human immunodeficiency virus activity; anticoagulant activity; antiretroviral properties; binding capacity; Biolayer interferometry; CD4 Antigens - immunology; Cell Line; Cluster of Differentiation 4 induced epitope; cytopathogenicity; cytotoxicity; dextran; Echinodermata; Echinodermata - chemistry; Envelope glycoprotein 120; epitopes; Epitopes - immunology; fucose; Fucose - chemistry; Glycosaminoglycan; Glycosaminoglycans - chemistry; heparin; HIV Envelope Protein gp120 - metabolism; HIV infections; HIV-1 - drug effects; HIV-1 - physiology; Human immunodeficiency virus 1; Humans; interferometry; Molecular Weight; mononuclear leukocytes; Structure-Activity Relationship; structure-activity relationships; sulfates; Virus Replication - drug effects; HIV-1; FG; dFG; Mw; SA; Biolayer interferometry; APTT; Envelope glycoprotein 120; AR2G; Anti-human immunodeficiency virus activity; Glycosaminoglycan; Cluster of Differentiation 4 induced epitope; BLI; Structure–activity relationship; fucosylated glycosaminoglycan; molecular weight; human immunodeficiency virus type 1; Amine Reactive Second-Generation; activated partial thromboplastin time; depolymerized fucosylated glycosaminoglycan; streptavidin biosensors

Fucosylated glycosaminoglycan (FG) is a novel glycosaminoglycan with a chondroitin sulfate-like backbone and fucose sulfate branches. The aim of this study is to investigate the mechanism and structure–activity relationships (SAR) of FG for combating HIV-1 infection. Anti-HIV activities of FGs were...