Transcriptome profiling of fast/glycolytic and slow/oxidative muscle fibers in aging and obesity

• Published in: Cell death & disease Vol. 15; no. 6; pp. 459 - 12
• Main Authors: Zhang, Feng-Min, Wu, Hao-Fan, Wang, Ke-Fan, Yu, Ding-Ye, Zhang, Xian-Zhong, Ren, Qi, Chen, Wei-Zhe, Lin, Feng, Yu, Zhen, Zhuang, Cheng-Le
• Format: Journal Article
• Language: English
• Published: London Nature Publishing Group UK 28.06.2024; Springer Nature B.V; Nature Publishing Group
• Subjects: 14/1; 14/19; 14/34; 14/63; 45; 45/91; 631/443/319/2723; 631/443/7; Aging; Aging - genetics; Aging - metabolism; Animals; Antibodies; Atrophy; Biochemistry; Biomedical and Life Sciences; Calcium signalling; Cell Biology; Cell Culture; CXCL10 protein; CXCL13 protein; Gene Expression Profiling; Glycolysis; Humans; Immunology; Life Sciences; Male; Mice; Mice, Inbred C57BL; Muscle Fibers, Skeletal - metabolism; Muscle Fibers, Slow-Twitch - metabolism; Muscles; Obesity; Obesity - genetics; Obesity - metabolism; Obesity - pathology; Public health; Thapsigargin; Transcriptome - genetics; Transcriptomes; Transcriptomics
• ISSN: 2041-4889; 2041-4889
• DOI: 10.1038/s41419-024-06851-y

Aging and obesity pose significant threats to public health and are major contributors to muscle atrophy. The trends in muscle fiber types under these conditions and the transcriptional differences between different muscle fiber types remain unclear. Here, we demonstrate distinct responses of fast/glycolytic fibers and slow/oxidative fibers to aging and obesity. We found that in muscles dominated by oxidative fibers, the proportion of oxidative fibers remains unchanged during aging and obesity. However, in muscles dominated by glycolytic fibers, despite the low content of oxidative fibers, a significant decrease in proportion of oxidative fibers was observed. Consistently, our study uncovered that during aging and obesity, fast/glycolytic fibers specifically increased the expression of genes associated with muscle atrophy and inflammation, including Dkk3, Ccl8, Cxcl10, Cxcl13, Fbxo32, Depp1, and Chac1, while slow/oxidative fibers exhibit elevated expression of antioxidant protein Nqo-1 and downregulation of Tfrc. Additionally, we noted substantial differences in the expression of calcium-related signaling pathways between fast/glycolytic fibers and slow/oxidative fibers in response to aging and obesity. Treatment with a calcium channel inhibitor thapsigargin significantly increased the abundance of oxidative fibers. Our study provides additional evidence to support the transcriptomic differences in muscle fiber types under pathophysiological conditions, thereby establishing a theoretical basis for modulating muscle fiber types in disease treatment.